PDF电子书：Protocols for Adult Stem Cells 2010(MiMB 621)

jimmy88cn

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这是2010出版的 Methods in Molecular Biology 系列 vol.621
" u0 e2 L+ B' J; M- K5 V与本站朋友分享。
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以下为该书前言：
" O' Y2 ~$ D/ b* r# y2 m/ GAlthough embryonic stem cells currently enjoy the public limelight and show great promise$ e( T8 [4 a( T
for cell based medical therapies, it is the adult stem cells which are responsible for the1 q3 h0 c$ V1 H8 K/ V% ?
body’s natural ability to fight disease, heal and recover, or fail and succumb to various: b( P# R# w# v! a$ w
maladies. The study of mammalian adult stem cells has surged recently, most likely from a. V6 e8 n* D' M' [4 t
maturation of stem cell studies in the classical developmental model organisms and in5 V4 ], f( t( z& o1 U
hematopoeisis. All the tissues of the body examined so far are generated and regenerated) N: @2 |% X1 h7 X  _
from stem cells, it has been an important first step to adapt or devise new methods to
, b/ i6 v" u# f& o! r8 tidentify and obtain these cells in quantity and purity for further study. Culture techniques, R1 y  V8 d$ R3 s. S0 ?6 h8 b
have been optimized for managing the growth and differentiation of stem cells in vitro; as
7 g: A9 [7 z1 ~' A! csome stem cells are pluripotent, often the method is to guide the fate of such cells among# h6 G% W6 ]4 w+ m0 e; C( a
the possible differentiation fates. Much of this work, and that in the classical model organisms,: x- c( b( r1 P' O2 V/ C
has helped define the aspects of the stem cell environment or niche that are crucial7 t1 B/ G4 N( m
for both growth and differentiation, and these studies have moved in vivo at increasingly/ E: K$ O1 V5 v, m
higher resolution. Importantly, the in vivo niche is a current target for bioengineering the$ Y) j* p/ y8 E) y) f
matrix and signaling factors.4 R. s6 E) e2 Q$ }: B8 w( u4 W
Herein, we present methods for studying six types of mammalian stem cells, mammary,% n/ p# W" w) ]# H, N
neural, mesenchymal, endothelial, dendritic, and muscle. We hope the approaches
: w+ l' ^1 D' T# B) V9 Zdescribed in these chapters will assist you in obtaining, characterizing, and studying these8 p( B- D& ]' p
cells or for adaptation to your favorite stem cells. One final note, should you find the" N$ J7 L8 Z2 T9 S! B! r
information herein valuable and it helps you to make great discoveries in the understanding# \" i6 G: t; U/ W
of stem cell biology or application, by all means please let us, the authors, know! The6 A" o' z& a' N. Y" A2 b2 }* L
best way is of course to publish your findings, but falling short of that, please feel free to" \' R, G/ `( B# J- X" M
ask questions or clarifications. Especially for this reason, our contact information is provided
! _3 _. v, _" M4 A" v* z# _at the end of this book.1 n0 f$ }4 f8 }, o2 G
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附件为ｐｄｆ全文　，回复本贴就可以下载了& P% H1 B& v+ c
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