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Application：Recommended for use as a cell culture substratum. For a 24-well plate, use 230-250 μl/well. For a 96-well plate, use 50-100 μl/well. Thaw gel overnight at 2-8 °C before use. The thawed gel may be diluted up to two-fold with cold (2-8 °C) Dulbecco′s Modified Eagle′s Medium. Gel dilutions should be made before it is added to the plate. ECM will gel within 5 minutes at 20 °C. For prolonged manipulations, work should be conducted below 10 °C. Dispense gel to wells of a multiwell plate using pipettes pre-cooled to 2-8 °C. A gel forms at 37 °C and maintains this form with culture medium for at least 14 days. Cells may be plated on top of a thin gel layer (0.5 mm) or cultured inside a 1 mm layer. When cultured inside, cells should be added to the gel prior to plating at a recommended density of 3-4 × 104 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml. 0 q3 O5 i/ ~8 W+ ]
  Epithelial cells, endothelial cells, muscle cells, nerve cells, tumor cells
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Caution：ECM gel may be stored up to 72 hours at 2-8 °C.( j! L( M9 H9 a$ g$ E5 T6 l- x# b+ M
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Other Notes：ECM gel is composed primarily of laminin, collagen type IV, heparan sulfate proteoglycan and entactin. Approximately 8-12 mg/ml basement membrane matrix protein in Dulbecco′s modified Eagle′s medium with 50 μg/ml gentamicin.
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Properties3 n, a- T. G/ v7 n. T) c
sterility dialyzed against chloroform $ @. G# @1 Q& \9 U9 j* h! ~
form liquid : `; p4 o4 Q! e+ l0 J2 o6 {$ L
concentration 8 - 12 mg/mL
, K; ]' J5 n# Z; J6 xsurface coverage 6‑10 μg/cm2
0 s; k" z% \& H" I3 B& Ktotal impurities endotoxin, tested
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shipped in dry ice
6 M1 r/ W% ^$ T" O* T# o0 Estorage temp. −20°C