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Application：Recommended for use as a cell culture substratum. For a 24-well plate, use 230-250 μl/well. For a 96-well plate, use 50-100 μl/well. Thaw gel overnight at 2-8 °C before use. The thawed gel may be diluted up to two-fold with cold (2-8 °C) Dulbecco′s Modified Eagle′s Medium. Gel dilutions should be made before it is added to the plate. ECM will gel within 5 minutes at 20 °C. For prolonged manipulations, work should be conducted below 10 °C. Dispense gel to wells of a multiwell plate using pipettes pre-cooled to 2-8 °C. A gel forms at 37 °C and maintains this form with culture medium for at least 14 days. Cells may be plated on top of a thin gel layer (0.5 mm) or cultured inside a 1 mm layer. When cultured inside, cells should be added to the gel prior to plating at a recommended density of 3-4 × 104 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml.
, p, x" M3 u* [2 P8 s& L Epithelial cells, endothelial cells, muscle cells, nerve cells, tumor cells 1 }2 P/ U: ?& M6 w& f
4 n- y& H3 `7 sCaution：ECM gel may be stored up to 72 hours at 2-8 °C.
! C% I. s; ]6 F- u- _* v2 j1 {2 D& D8 w: v' L9 I
Other Notes：ECM gel is composed primarily of laminin, collagen type IV, heparan sulfate proteoglycan and entactin. Approximately 8-12 mg/ml basement membrane matrix protein in Dulbecco′s modified Eagle′s medium with 50 μg/ml gentamicin.0 c2 ^& b# _1 i' A. x; A6 t
. G; W1 T- p; b4 q4 r
Properties# e$ s1 ~! W" X, Q* `/ x7 d' W/ v
sterility dialyzed against chloroform . ?( P6 Y9 `; w" a
form liquid 5 O/ Q7 i; ? ~: N
concentration 8 - 12 mg/mL / N3 _! t; m5 @( J, U, o. L/ m* `
surface coverage 6‑10 μg/cm2
. p- f2 \ m( ? gtotal impurities endotoxin, tested 3 r' n0 V0 q: ~1 ^2 P
suitability cell culture tested
" @. x& i) a1 M( F6 O* ]& R: E* r/ Ishipped in dry ice
( X m& d0 c" A! A3 t; wstorage temp. −20°C |
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总评分: 威望 + 5
包包 + 10
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发表于 2010-8-11 10:46
