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- 积分
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- 威望
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- 包包
- 697
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Application：Recommended for use as a cell culture substratum. For a 24-well plate, use 230-250 μl/well. For a 96-well plate, use 50-100 μl/well. Thaw gel overnight at 2-8 °C before use. The thawed gel may be diluted up to two-fold with cold (2-8 °C) Dulbecco′s Modified Eagle′s Medium. Gel dilutions should be made before it is added to the plate. ECM will gel within 5 minutes at 20 °C. For prolonged manipulations, work should be conducted below 10 °C. Dispense gel to wells of a multiwell plate using pipettes pre-cooled to 2-8 °C. A gel forms at 37 °C and maintains this form with culture medium for at least 14 days. Cells may be plated on top of a thin gel layer (0.5 mm) or cultured inside a 1 mm layer. When cultured inside, cells should be added to the gel prior to plating at a recommended density of 3-4 × 104 cells per mL. To dissociate cells from the gel, use protease (dispase) dissolved in PBS without calcium, magnesium, and EDTA at a working concentration of 0.6-2.4 units/ml.
! q5 }. J5 K. @ Epithelial cells, endothelial cells, muscle cells, nerve cells, tumor cells $ y* R4 p4 d; H
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Caution：ECM gel may be stored up to 72 hours at 2-8 °C." D: \1 p, \% }3 t7 L
& _( Z) G/ X% ^2 C) r# aOther Notes：ECM gel is composed primarily of laminin, collagen type IV, heparan sulfate proteoglycan and entactin. Approximately 8-12 mg/ml basement membrane matrix protein in Dulbecco′s modified Eagle′s medium with 50 μg/ml gentamicin.
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5 K/ I' [) j' d% P4 Z% cProperties. ^1 o$ y) d% V. R2 }7 |
sterility dialyzed against chloroform
. s6 ?' C9 e+ f/ q7 e2 g9 nform liquid 6 ]3 Z9 Z" V3 @- s+ C- M+ t
concentration 8 - 12 mg/mL
/ v9 z3 q5 ~+ H6 \% k, R: Q# D7 Qsurface coverage 6‑10 μg/cm2 . X+ S( V7 m2 c3 V c0 D$ o& u
total impurities endotoxin, tested ( f& B5 f- F" x" w7 G+ c* z' l0 o' H7 J
suitability cell culture tested + d: X. E7 ^9 N9 f
shipped in dry ice
$ j# b( x* L6 pstorage temp. −20°C |
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总评分: 威望 + 5
包包 + 10
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发表于 2010-8-11 10:46
