最新综述DNA methylation and cellular reprogramming

shenven

DNA methylation and cellular reprogramming.pdf( w: R7 S- I5 w# k% k

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The recent discovery that a small number of defined
5 {5 i; Z1 I# G  vfactors are sufficient to reprogram somatic cells into7 n5 n+ C5 r# L; A3 B' E
pluripotent stem cells has significantly expanded our* K. K' v, }6 j$ P0 `
knowledge of the plasticity of the epigenome. In this
! m6 Q2 f2 [+ s0 oreview we discuss some aspects of cell fate plasticity and
3 \* K4 l7 p) K8 H' x# Mepigenetic alterations, with emphasis on DNA methylation: ?3 n/ |2 b0 M' m
during cellular reprogramming. Recent data suggest
1 P' q7 _8 H' _! s0 ]* ~* xthat DNA methylation is a major barrier to induced: L9 d, I9 W. m
pluripotent stem (iPS) cell reprogramming. The' {# a) K2 N+ Y0 x" ~: t
demethylating agent 5-azacytidine can enhance the efficiency
6 g7 b% N, q& u: K- ~7 b) k5 Iof iPS cells generation and the putative DNA
: O" m1 P/ C0 l7 D* ?8 kdemethylase protein activation-induced cytidine deaminase
; Q7 Q: \$ a" @2 X6 V( c8 L(AID/AICDA) can erase DNA methylation at pluripotency8 W$ B/ I! Z7 D1 O
gene promoters, thereby allowing cellular$ e4 [6 X4 t6 U. u: x" x
reprogramming. Elucidation of the epigenetic changes' M  Y, n+ i: d, Q
taking place during cellular reprogramming will enhance0 D# Y0 R# \  v9 t# Y) u9 @& X( J
our understanding of stem cell biology and facilitate% h! |0 B6 `# Z: {. d
therapeutic applications.

swxxx

不错

tspaulzhao

Good job. Thanks.