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本帖最后由 张也行 于 2010-10-26 01:06 编辑 e2 h1 E- H$ z
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1. Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos
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% X# K$ @, N: L6 vNature Protocols 1, 1145 - 1153 (2006) / [3 Q) N2 R# o5 _
Published online: 7 September 2006 | doi:10.1038/nprot.2006.173
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2. Production of mice using iPS cells and tetraploid complementation
9 E: o( D5 U3 eNature Protocols 5, 963–971 (1 May 2010) | doi:10.1038/nprot.2010.61
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" ^7 z, t9 T3 a$ }1 f8 N8 phttp://www.nature.com/nprot/journal/v5/n5/full/nprot.2010.61.html
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3. Generation of induced pluripotent stem cells from human cord blood cells with only two factors: Oct4 and Sox2
4 w' U# }/ D% `7 u! U- H! _Nature Protocols 5, 811–820 (1 April 2010) | doi:10.1038/nprot.2010.16 : y/ ]4 u7 n3 j* W" E8 C
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http://www.nature.com/nprot/journal/v5/n4/full/nprot.2010.16.html
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3 I) ?( s4 p) }# u/ l& ]4. A protocol describing the genetic correction of somatic human cells and subsequent generation of iPS cells e7 d! m7 u9 d% |$ l9 J K' I
Nature Protocols 5, 647–660 (1 April 2010) | doi:10.1038/nprot.2010.9
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5. An efficient and easy-to-use cryopreservation protocol for human ES and iPS cells
# U3 F. E9 l F# L% E. @; mNature Protocols 5, 588–594 (1 March 2010) | doi:10.1038/nprot.2009.247
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6. EOS lentiviral vector selection system for human induced pluripotent stem cells
8 S e$ q7 I0 QNature Protocols 4, 1828 - 1844 (2009)
$ n# {6 I7 \0 G* i0 `# k3 m* _: QPublished online: 3 December 2009 | doi:10.1038/nprot.2009.201/ E! C/ E' f- ~: L8 ^
+ A7 ], A5 W* E* Qhttp://www.nature.com/nprot/jour ... nprot.2009.201.html
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7. Protocol for the fast chromatin immunoprecipitation (ChIP) method
* `4 u5 D' B+ @/ S* _Nature Protocols 1, 179 - 185 (2006)
( G/ m% C% _( s$ v, A- \! aPublished online: 27 June 2006 | doi:10.1038/nprot.2006.27
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http://www.nature.com/nprot/journal/v1/n1/full/nprot.2006.27.html2 U2 f9 B( b5 ]3 l
3 }+ J! Q( x) K6 A( D, M8. Changing medium and passaging cell lines
, X, s* a, L) c! @* w* P8 C: pNature Protocols 2, 2276 - 2284 (2007)
7 I, b. g9 g9 KPublished online: 13 September 2007 | doi:10.1038/nprot.2007.319
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) C' z/ ]* o# Z8 F! K3 u9. Derivation of human embryonic stem cells from single blastomeres$ {5 O. s) F, c2 ?
Nature Protocols 2, 1963 - 1972 (2007)
" z2 `6 W Y' `7 ?( |% bPublished online: 9 August 2007 | doi:10.1038/nprot.2007.274
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http://www.nature.com/nprot/journal/v2/n8/pdf/nprot.2007.274.pdf) g: `( E: C0 S( O: M1 B8 W. W
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10. Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro" D8 n) i5 p Q; J# g& ~. P$ r
Nature Protocols 3, - 1046 - 1055 (2008)
& z Y9 g. l% E5 Q* R8 _ F B! bPublished online: 29 May 2008 | doi:10.1038/nprot.2008.698 k) P* Q* f+ q% W2 [7 o: g9 x
) C- [+ z! k9 c7 R" u( v8 z" Ohttp://www.nature.com/nprot/journal/v3/n6/abs/nprot.2008.69.html& K/ k& H2 }( M
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实在不好意思,我们图书馆没有Nature protocol的数据库,麻烦各位了!谢谢! |
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