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本帖最后由 张也行 于 2010-10-26 01:06 编辑 8 e- c7 b6 {+ r) m
/ E( p$ l- d; h6 x1 g0 I7 g v h1. Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos
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! t* G% o$ V3 v* ?& I8 \Nature Protocols 1, 1145 - 1153 (2006) r; C& a) F4 G4 I$ z0 V9 n0 t
Published online: 7 September 2006 | doi:10.1038/nprot.2006.1739 L% J% K! X! s9 }0 I; x4 [
连接:http://www.nature.com/nprot/jour ... nprot.2006.173.html. z4 ?' ~# e2 v4 [
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2. Production of mice using iPS cells and tetraploid complementation4 P, N) |0 x4 W7 T5 x0 B! I
Nature Protocols 5, 963–971 (1 May 2010) | doi:10.1038/nprot.2010.61 ! j- F' @* q6 u! J; Z" Q
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http://www.nature.com/nprot/journal/v5/n5/full/nprot.2010.61.html
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& f$ V3 s1 s; h+ F$ l3. Generation of induced pluripotent stem cells from human cord blood cells with only two factors: Oct4 and Sox2
, [: t' ~8 D$ o+ R0 H9 MNature Protocols 5, 811–820 (1 April 2010) | doi:10.1038/nprot.2010.16
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! g9 d& G0 Y, f1 U/ A+ Nhttp://www.nature.com/nprot/journal/v5/n4/full/nprot.2010.16.html; V6 g: }9 z9 K$ x# r2 q
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4. A protocol describing the genetic correction of somatic human cells and subsequent generation of iPS cells0 |& N5 U2 i: M# f
Nature Protocols 5, 647–660 (1 April 2010) | doi:10.1038/nprot.2010.9, }0 I$ h8 r6 d8 p
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http://www.nature.com/nprot/journal/v5/n4/full/nprot.2010.9.html
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- J( @0 C2 n7 ~: }/ A i9 p4 y+ J5. An efficient and easy-to-use cryopreservation protocol for human ES and iPS cells
+ X& g: O* d2 C }9 j# F! O- BNature Protocols 5, 588–594 (1 March 2010) | doi:10.1038/nprot.2009.247
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& d' G5 @6 }1 ]4 T) I6 ihttp://www.nature.com/nprot/jour ... nprot.2009.247.html
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2 [, R7 q; _ k/ h8 S) ~; V6. EOS lentiviral vector selection system for human induced pluripotent stem cells( l x( k9 M3 l0 }
Nature Protocols 4, 1828 - 1844 (2009) + U$ H( E" R. P7 J, r# B& J6 g" ?: P
Published online: 3 December 2009 | doi:10.1038/nprot.2009.2011 F! Z# j3 M& t& ?8 ]
, ~2 j9 @, Q7 C1 v: Dhttp://www.nature.com/nprot/jour ... nprot.2009.201.html
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* ?3 w# X( T; ~5 @) ^7. Protocol for the fast chromatin immunoprecipitation (ChIP) method
& S6 `8 C8 Y) H4 G1 TNature Protocols 1, 179 - 185 (2006)
. b6 k5 J+ Q3 NPublished online: 27 June 2006 | doi:10.1038/nprot.2006.270 @8 b9 Q% D. C* U' N8 p& o. `
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http://www.nature.com/nprot/journal/v1/n1/full/nprot.2006.27.html8 h" W& Y) ]3 Y3 D2 q
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8. Changing medium and passaging cell lines
9 d \ `" A1 ^! C2 x" fNature Protocols 2, 2276 - 2284 (2007)
b: p$ l" m9 P- ZPublished online: 13 September 2007 | doi:10.1038/nprot.2007.3190 u1 N* F* d& j* o# m4 s# F4 }
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http://www.nature.com/nprot/jour ... nprot.2007.319.html
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9. Derivation of human embryonic stem cells from single blastomeres
4 a+ }3 s) P$ a4 O9 j3 B3 ANature Protocols 2, 1963 - 1972 (2007) + o4 ^* X2 H) j, p6 X
Published online: 9 August 2007 | doi:10.1038/nprot.2007.274
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http://www.nature.com/nprot/journal/v2/n8/pdf/nprot.2007.274.pdf
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10. Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro9 Z; a- ~: w$ o4 m- d
Nature Protocols 3, - 1046 - 1055 (2008)
. F2 P. Z6 j: w7 w$ U- F5 \Published online: 29 May 2008 | doi:10.1038/nprot.2008.69- g6 B& \- Q+ j
! _! h; \* D$ I( Thttp://www.nature.com/nprot/journal/v3/n6/abs/nprot.2008.69.html& i# M6 l8 u5 M( W; X. h
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' K! K) F" O- x5 n0 z; Q9 k实在不好意思,我们图书馆没有Nature protocol的数据库,麻烦各位了!谢谢! |
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