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本帖最后由 张也行 于 2010-10-26 01:06 编辑 " y" C/ J# Q4 f, M% z9 ^
: x1 O6 {6 y0 e3 Q0 @5 h' y6 ^1. Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos
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# I3 o1 Q# e* u$ L. U, ~2 o+ ~Nature Protocols 1, 1145 - 1153 (2006) ! x$ G! Q5 l& l9 p) J; R
Published online: 7 September 2006 | doi:10.1038/nprot.2006.173
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2. Production of mice using iPS cells and tetraploid complementation8 ]/ N2 J1 O- w( h
Nature Protocols 5, 963–971 (1 May 2010) | doi:10.1038/nprot.2010.61
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: f* Q2 E4 h9 [/ v$ chttp://www.nature.com/nprot/journal/v5/n5/full/nprot.2010.61.html
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3. Generation of induced pluripotent stem cells from human cord blood cells with only two factors: Oct4 and Sox2
) R6 l; C! m% m+ S* x" B7 PNature Protocols 5, 811–820 (1 April 2010) | doi:10.1038/nprot.2010.16 * b. f! E, `1 a; c
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http://www.nature.com/nprot/journal/v5/n4/full/nprot.2010.16.html# y2 @, S- w1 [- e) `
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4. A protocol describing the genetic correction of somatic human cells and subsequent generation of iPS cells8 @. h4 U2 M1 z# r7 D: c0 R: \9 G
Nature Protocols 5, 647–660 (1 April 2010) | doi:10.1038/nprot.2010.9
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2 [) m4 G {4 u2 i6 T3 z5. An efficient and easy-to-use cryopreservation protocol for human ES and iPS cells. U% V! D; m% g! d0 G: [/ U: |
Nature Protocols 5, 588–594 (1 March 2010) | doi:10.1038/nprot.2009.247 * ~- c% T' S# _+ J+ b& I# E
4 U$ j' V4 L$ mhttp://www.nature.com/nprot/jour ... nprot.2009.247.html
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+ r! e1 d* @5 H# b6 {6. EOS lentiviral vector selection system for human induced pluripotent stem cells* {. W" f' Y* l( j
Nature Protocols 4, 1828 - 1844 (2009)
" g# M( Y% ^4 y0 r+ F; iPublished online: 3 December 2009 | doi:10.1038/nprot.2009.201
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7. Protocol for the fast chromatin immunoprecipitation (ChIP) method
$ D: N$ b' o& p4 L0 ?: |0 FNature Protocols 1, 179 - 185 (2006)
: o/ w% |4 B4 t- G& s. ~Published online: 27 June 2006 | doi:10.1038/nprot.2006.27, ^" V8 C- y+ @
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http://www.nature.com/nprot/journal/v1/n1/full/nprot.2006.27.html
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$ U6 j- g' d$ l+ r/ F2 O; O0 {8. Changing medium and passaging cell lines
2 K% ]0 G, c! Z9 MNature Protocols 2, 2276 - 2284 (2007)
6 ~. ~1 H9 t7 h/ E, n' BPublished online: 13 September 2007 | doi:10.1038/nprot.2007.319/ @. F+ y+ M8 z& N( y* t
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http://www.nature.com/nprot/jour ... nprot.2007.319.html
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' T# i4 s+ y& E- h, ?( ]9. Derivation of human embryonic stem cells from single blastomeres
& ~# _4 x* V( S; F$ H( y/ o' KNature Protocols 2, 1963 - 1972 (2007) % w' @6 D, q3 T
Published online: 9 August 2007 | doi:10.1038/nprot.2007.2747 a4 y+ {- V; ?4 n: i
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http://www.nature.com/nprot/journal/v2/n8/pdf/nprot.2007.274.pdf* Y! b1 B1 [# X4 [$ q
+ H& t! Y( X: K% L10. Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro# w9 ?9 F& Q- t. Y5 B7 x9 _/ r
Nature Protocols 3, - 1046 - 1055 (2008)
) @1 s" }& T4 U6 j' @Published online: 29 May 2008 | doi:10.1038/nprot.2008.69( ]% q4 C- i- z% V: q$ O; ?" r
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http://www.nature.com/nprot/journal/v3/n6/abs/nprot.2008.69.html, s2 }- P: i4 m0 L' v K
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4 P1 W/ e( n- S, A, H6 G实在不好意思，我们图书馆没有Nature protocol的数据库，麻烦各位了！谢谢！ |
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发表于 2010-10-26 00:54
