Enzymatic-Mechanical Dissociation 分离效果怎么样？有人做过么？

西瓜太郎

2.4 Enzymatic-Mechanical Dissociation  A new method combining enzymatic digestion and mechanical dissociation was established. 8 O, T$ R1 P! h6 e4 n3 n
In brief, paste-like tissues were treated with 15ml enzyme mixture at 37o3 ?: e- B& A+ W; V
C for 30 minutes with gentle agitation (180rpm) to  lose  the collagen matrix. Enzymatic reaction was stopped
! m3 k* K. O( nby adding  an  equal volume of MSC complete media.  Mixture were mechanically + o" C2 T) {% y+ C# p0 w
homogenised by using hand held cell homogeniser (Wiggen Hauser, Germany) at 9000 rpm
, [" ]4 O, A3 b  t6 vfor 5 min  4  cycles. Mixture was then filtered by using 70µm and 40µm cell strainer to + c; l( V" ~+ O! l1 q/ e+ D+ \
exclude  the excessive tissue. The cells were washed, counted and seeded into 25cm2
3 ~+ a) m; T! K$ {" i( I  tissue culture flask in MSC complete media at 1x106cell/cm2" R& R  I% {) A( o# S8 f8 C$ @

: j& C, j; r7 R) D4 Z" ]结果：Enzymatic-mechanical dissociation method can provide constantly high
6 u4 M1 d, T2 C' ~% ^" ?5 ~mononuclear cell yield (84.65±12.60 x 106cells/cm, n=6) compared to enzymatic digestion 3 d* K" e$ D( ]/ Y
method (0.50±0.33 x 106 cells/cm, n=5) (Table 2). The successful rate of generation of MSC
0 Y& E5 W6 Y9 Rusing enzymatic-mechanical dissociation method are relatively higher (70%), followed  by
+ ~" w& {: F' Y% o2 E( sexplant method (25%) and enzymatic digestion (0%).  

西瓜太郎

不知道有大虾做过这个不？结果差别真的有这么大么

呼吸

我也想知道，脐带答案。