用表达骨形成蛋白2的自体骨髓间充质干细胞修复下颌牙槽骨缺损

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J Craniofac Surg. 2011 Mar 9. [Epub ahead of print]  
8 x# H# `0 a2 o' m- j0 O/ AMandibular Alveolar Bony Defect Repair Using Bone Morphogenetic Protein 2-Expressing Autologous Mesenchymal Stem Cells.% @0 d4 X$ n: F  q
Chung VH, Chen AY, Kwan CC, Chen PK, Chang SC.: U. G; W1 H$ `9 I/ U! M
From the *Department of Applied Chemistry, Providence University, Taichung, †Department of Dentistry, Chang-Gung Memorial Hospital, Taoyuan; ‡Department of Plastic Surgery, Chang Gung Memorial Hospital, Chang Gung University College of Medicine; Taoyuan; and §Department of Plastic Surgery and ∥School of Medicine, China Medical University, Taichung,Taiwan.9 u3 _# i- X, L6 t8 v* g1 x
Abstract
0 Y( I; D7 ?+ r; O% MBACKGROUND: Mandibular bone regeneration is stepped up by human recombinant bone morphogenetic protein 2 (BMP-2) whose application is also related to limited cementum and periodontal ligament regeneration, local root resorption, and ankylosis. The alveolar bone grafting without traditional autologous bone grafts remains a challenge for plastic surgeons.8 J& y0 z1 x# C9 ]
METHODS: Bilateral mandibular alveolar and periodontal defects were created over the premolar areas in 9 mature male beagles. The defects were randomly assigned for either the adenovirus BMP-2 (advBMP-2) group with BMP-2-expressing mesenchymal stem cells (MSCs) or the control with MSCs alone. The regenerated periodontal attachment apparatus was evaluated histologically, and the whole regenerated bone volume was scrutinized from three-dimensional computed tomography analysis.$ v/ y4 S0 G  j9 V  }' U
RESULTS: Periodontal apparatus regeneration was significantly better in the advBMP-2 group. New cementum and Sharpey fibers were observed on the denuded root surfaces in the advBMP-2 group, whereas incomplete healing with localized root surface resorption was noted in the control group. Eight weeks after implantation, the advBMP-2 group showed significant increase in bone regeneration than the control one.
) b" Z5 M0 ]# D, ^1 w' nCONCLUSIONS: Thus, the use of ex vivo BMP-2-engineered autologous MSCs boosted bone and periodontal apparatus regeneration in mandibular periodontal defects. This de novo approach might be suitable for clinical mandibular bone repair and periodontal apparatus repair.0 ?* Y3 ]2 p# |) P: L3 {
PMID: 21403565 [PubMed - as supplied by publisher]
  ~+ I# A; ]8 R: d/ u/ j8 rRelated citations
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' D! M! ?, Z% c  M2 g' p

2 w0 t: C6 G' w  r 颅面外科杂志  2011-3-9  付印前电子版& O' G' s( e. x  A0 A/ \
用表达骨形成蛋白2的自体骨髓间充质干细胞修复下颌牙槽骨缺损
9 V9 x5 }) H/ |7 i) KChung VH, Chen AY, Kwan CC, Chen PK, Chang SC." g& t3 M5 [# V; B  E
台中市 静宜大学 应用化学系；桃园 长庚纪念医院 口腔科；桃园 张长庚大学医学院 长庚医院 整形外科；台湾 台中市 中国医药大学 医学院 整形外科系* c. F+ Z3 D  z" x& _
摘要2 y- l( i$ V1 W2 I4 U
背景: 人重组骨形成蛋白2加速了下颌骨的再生并对牙骨质和牙周膜的再生、局部牙根吸收及关节强直有一定作用。不用传统的自体骨移植物修复牙槽骨对整形外科来说仍是一个挑战。
: }/ Y2 S$ h6 w! H& k: p4 J* w方法: 在9只成年雄性贝格犬的整个前磨牙区域构建双侧下颌骨牙槽和牙周缺损。将缺损模型随机分到含表达骨形成蛋白-2的间充质干细胞的腺病毒骨形成蛋白-2组或单纯间充质干细胞对照组。组织学评估再生的牙周附着组织并通过三维计算机断层扫描分析检查全部再生的骨量。
! M! C  |  Y. B5 E" Y4 Z. z- k结果:观察腺病毒骨形成蛋白-2组中裸露牙根表面的新牙骨质和沙比纤维，发现腺病毒骨形成蛋白-2组的牙周膜组织再生明显更多而对照组呈现伴牙根表面吸收的不完全愈合。移植8周后腺病毒骨形成蛋白-2组的骨再生显著多于对照组。9 ]9 h' ]4 J) {# H! ^2 K
结论: 因此用体外骨形成蛋白-2-工程的自体间充质干细胞 (这句话没完全理解，望大侠们指教)提高了下颌牙周缺损中骨和牙周组织再生。这种新方法可能适合临床下颌骨修复和牙周组织修复。% ?" K. c& C  G2 z" j; k2 n9 W

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谢谢前辈，我一下转不过弯来，呵呵，正在改进中……0 F9 K% e+ c1 {; B. _7 Z
修正后译文如下：
, Z3 z2 Q) ^* \1 b& ]+ W颅面外科杂志  2011-3-9  付印前电子版
9 M1 {8 I% k; ]0 r( Q( M1 c用表达骨形成蛋白2的自体骨髓间充质干细胞修复下颌牙槽骨缺损
$ _4 T% r* _1 E. j; y6 y- K" XChung VH, Chen AY, Kwan CC, Chen PK, Chang SC.
& y7 L7 |" `/ k, M9 W台中市 静宜大学 应用化学系；桃园 长庚纪念医院 口腔科；桃园 张长庚大学医学院 长庚医院 整形外科；台湾 台中市 中国医药大学 医学院 整形外科系3 R. x3 P9 [+ ?; d2 m3 T
摘要: H- ^) k, R' H. _! {
背景: 人重组骨形成蛋白2加速了下颌骨的再生并对牙骨质和牙周膜的再生、局部牙根吸收及关节强直有一定作用。不用传统的自体骨移植物修复牙槽骨对整形外科来说仍是一个挑战。+ _# e7 f5 v( |* M5 e- J% ]2 t
方法: 在9只成年雄性贝格犬的整个前磨牙区域构建双侧下颌骨牙槽和牙周缺损。将缺损模型随机分到含表达骨形成蛋白-2的间充质干细胞的腺病毒骨形成蛋白-2组或单纯间充质干细胞对照组。组织学评估再生的牙周附着组织并通过三维计算机断层扫描分析检查全部再生的骨量。
+ Q6 V% D" l; A1 q: i4 R结果:观察腺病毒骨形成蛋白-2组中裸露牙根表面的新牙骨质和沙比纤维，发现腺病毒骨形成蛋白-2组的牙周膜组织再生明显更多而对照组呈现伴牙根表面吸收的不完全愈合。移植8周后腺病毒骨形成蛋白-2组的骨再生显著多于对照组。
' j7 j' N4 y" ~& z- V  D9 [  C结论: 因此用成骨蛋白体外改造的自体间充质干细胞提高了下颌牙周缺损中骨和牙周组织再生。这种新方法可能适合临床下颌骨修复和牙周组织修复。( ]' Q: Y7 P0 G8 S) y( c