Hank’s buffer： : a& b2 \/ r& `9 `4 A" K5 q ; F. r. R' ^! u# r/ J" |137 mM NaCl; / w( J w3 J8 N# l4 R) f
5.4 mM KCl; ! e. K m+ E; R7 {" g
0.95 mM CaCl2; ( X" p( N- R6 |) g( q) K0.41 mM MgSO4; ! q# |1 L0 }. B5 L9 D2 w$ d
0.49 mM MgCl2; - C J2 u( X' V0 U7 L7 G
4.17 mM NaHCO3; 4 S$ h1 S" y4 l* x; d5 a( Z* @
0.34 mM Na2HPO4; / Y/ L, E9 e( Q7 I. o
0.44 mM KH2PO4; : E: G0 M$ O. r& Q20 mM HEPES, pH 7.2 : ?5 t- ]2 p1 {/ S% b& j! D
+ w: P3 N$ F3 j0 k可以直接购买来自HyClone Hanks' Balanced Salt Solutions(HBSS)。 1 H5 v. C8 ~2 C( d* n, ^) d来自文章---Efficient expression of foreign genes in CHO DHFR(-) cells by electroporation
Optimization of square-wave electroporation for transfection of porcine fetal fibroblasts7 }6 F0 I& J2 C" ~! E3 d
这篇文章里面用的是混合配方: 8 B. @2 p& G$ v+ h* \) [1 ECells were resuspended in transfection media (75% cytosalts [120 mM KCl, 0.15 mM CaCl2, 10 mM K2HPO4; pH 7.6, 5 mM MgCl2] (van den Hoff et al.1992) and 25% Opti-MEM) ( G" F5 M& I3 ?$ n9 l Z0 P6 B 3 T1 o, H4 J$ Y4 F/ Y
发表于 2012-5-28 20:21
