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本帖最后由 细胞海洋 于 2011-5-15 23:33 编辑
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Table of Contents2 u! @9 m |" ^+ D" s- x6 w
Chapter 1: Plasmids and Their Usefulness in Molecular Cloning
% ?/ A0 N2 p% QChapter 2: Bacteriophage and Its Vectors6 K* I2 d% ~3 G0 e& i& D
Chapter 3: Working with Bacteriophage M13 Vectors% r. S0 m; n) ?2 P1 d
Chapter 4: Working with High-Capacity Vectors
" _0 |1 M$ p c0 M; wChapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose
/ a, H$ l2 R4 \8 q( s7 y/ DChapter 6: Preparation and Analysis of Eukaryotic Genomic DNA; I" i6 P- n4 n- ?
Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells0 z, ]9 r: V9 y( q- f9 u
Chapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction
2 W/ t1 n6 {' V3 E0 I' U, I) XChapter 9: Preparation of Radiolabeled DNA and RNA Probes
9 H+ W1 n# q8 D; ]Chapter 10: Working with Synthetic Oligonucleotide Probes
7 Q- e' _# \% E! ^Chapter 11: Preparation of cDNA Libraries and Gene Identification: f( B0 r. d) s
Chapter 12: DNA Sequencing
% B( S9 r" j7 P3 yChapter 13: Mutagenesis# `/ C) x1 B+ b
Chapter 14: Screening Expression Libraries
& G/ t' g4 U7 I) LChapter 15: Expression of Cloned Genes in Escherichia coli6 a* E. u* U1 c, l7 n( K' ?
Chapter 16: Introducing Cloned Genes into Cultured Mammalian Cells1 G4 X& [) ^; \9 ?3 ^
Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells
% p: K( n. \4 Z5 ^2 e3 KChapter 18: Protein Interaction Technologies
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