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本帖最后由 细胞海洋 于 2011-5-15 23:33 编辑
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0 z8 h$ u9 W& O4 B+ O" nTable of Contents3 |- c ?, O& I5 n0 C
Chapter 1: Plasmids and Their Usefulness in Molecular Cloning* N! m1 r4 n# f
Chapter 2: Bacteriophage and Its Vectors
. I8 h9 g* k" }* ?2 Q% A6 vChapter 3: Working with Bacteriophage M13 Vectors
- ]9 L5 b: A3 ~Chapter 4: Working with High-Capacity Vectors: L1 T9 Z, G( E: o1 i9 J4 u
Chapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose
: X; U2 w0 u0 F2 w1 aChapter 6: Preparation and Analysis of Eukaryotic Genomic DNA$ q" c4 e: K4 p' Z# y- o
Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
2 d" p8 B6 t$ @! i; R+ IChapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction7 ^* q. R) p7 e5 F1 [8 ?8 U
Chapter 9: Preparation of Radiolabeled DNA and RNA Probes
h, g3 k# p: A% U: M2 iChapter 10: Working with Synthetic Oligonucleotide Probes
7 x1 {0 @; n5 F6 @Chapter 11: Preparation of cDNA Libraries and Gene Identification
; I7 z' q7 ?3 I; ~4 M- vChapter 12: DNA Sequencing
9 Y( ^9 U2 ?- k N0 oChapter 13: Mutagenesis
7 o" d. `& F l2 ^Chapter 14: Screening Expression Libraries
# k1 [+ I' n4 a$ \3 X5 ` r8 T4 C9 [Chapter 15: Expression of Cloned Genes in Escherichia coli
) A. f6 e E o4 wChapter 16: Introducing Cloned Genes into Cultured Mammalian Cells
4 j4 b0 I$ w1 l: UChapter 17: Analysis of Gene Expression in Cultured Mammalian Cells
* i: t* F# p% l8 NChapter 18: Protein Interaction Technologies
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