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本帖最后由 细胞海洋 于 2011-5-15 23:33 编辑
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! g( S# j" c# L& n% x4 f2 mTable of Contents" f% Z, H% a7 ~) ?8 k O
Chapter 1: Plasmids and Their Usefulness in Molecular Cloning
- K& x! e3 @1 L# d1 N- m9 |+ aChapter 2: Bacteriophage and Its Vectors3 Q- b: f- ]% n4 n
Chapter 3: Working with Bacteriophage M13 Vectors% m1 e G" `2 x5 O" H0 h
Chapter 4: Working with High-Capacity Vectors- q0 y0 X2 ?. N/ U1 a' B: O6 S) i
Chapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose
) _$ R$ ?( @& N5 _) B/ ?' y0 LChapter 6: Preparation and Analysis of Eukaryotic Genomic DNA
& A, I e1 j6 ]/ B: JChapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells1 W0 }5 F. {1 ~
Chapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction
1 _" ?" J* N* T! ?Chapter 9: Preparation of Radiolabeled DNA and RNA Probes, F- N) a; I* f
Chapter 10: Working with Synthetic Oligonucleotide Probes* {9 f2 I7 }7 T6 B$ M# y
Chapter 11: Preparation of cDNA Libraries and Gene Identification' {0 W! ?9 G" q/ d7 L
Chapter 12: DNA Sequencing
; n. w4 ^/ i& X% W+ R! Q# K% ^# kChapter 13: Mutagenesis
: O+ @# A9 o( C7 V" tChapter 14: Screening Expression Libraries, \6 ~ g Q0 r) C7 V
Chapter 15: Expression of Cloned Genes in Escherichia coli
+ t) t9 H! L' Q. z$ C. `: V3 wChapter 16: Introducing Cloned Genes into Cultured Mammalian Cells! f! H0 X; ]0 [# @4 f
Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells
- b( }' y2 @9 h+ N. tChapter 18: Protein Interaction Technologies$ o b7 `0 I4 \; B. Z+ i5 V
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