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本帖最后由 细胞海洋 于 2011-5-15 23:33 编辑 8 U4 z: H/ B. Q2 X5 F
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Table of Contents
+ d* `/ X7 u: y+ }6 L; M2 ~Chapter 1: Plasmids and Their Usefulness in Molecular Cloning. I9 r' C& @* }5 T/ e; u
Chapter 2: Bacteriophage and Its Vectors
* N+ s Z6 l6 [3 o( `$ N9 KChapter 3: Working with Bacteriophage M13 Vectors
6 |' T4 e9 B' t* s& T0 g+ g! `Chapter 4: Working with High-Capacity Vectors
' S8 k9 S q& S+ E5 qChapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose
2 H% W: `. O% a* j/ Y% `Chapter 6: Preparation and Analysis of Eukaryotic Genomic DNA
' O4 k. h8 v( e+ I: w& _+ D/ UChapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells' ~$ O9 a0 o" N' e4 x$ [) v
Chapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction
. Y9 g6 O* ^( |0 Y$ _% GChapter 9: Preparation of Radiolabeled DNA and RNA Probes; [3 i0 Q7 w$ o* @9 o7 D6 @
Chapter 10: Working with Synthetic Oligonucleotide Probes
& z# O3 [; W. m) uChapter 11: Preparation of cDNA Libraries and Gene Identification! S+ U1 g" [0 I, v1 ]6 g
Chapter 12: DNA Sequencing
$ u$ J, H. \) Y- g8 m+ h7 OChapter 13: Mutagenesis
7 b; L; M8 j$ v5 WChapter 14: Screening Expression Libraries
% W+ @$ Q3 S2 A$ q- D5 n8 ^' WChapter 15: Expression of Cloned Genes in Escherichia coli# ?' Z& M1 x2 |6 J
Chapter 16: Introducing Cloned Genes into Cultured Mammalian Cells
* Q2 a- j7 n& D3 d( x5 R1 v% ]Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells. J9 M& i) X: _7 C# z$ P
Chapter 18: Protein Interaction Technologies( k$ K5 X9 s( a1 D4 p5 R
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