请教一下，大家将肿瘤干细胞分选出来后怎样抑制其分化？

conanthird

leisong12 发表于 2011-5-17 22:37
! @: Z; V% L/ j* c8 m  E. _What is KSR, would you explain it in detail.

( r5 F) Y5 a1 [+ {* }; e* gKnockOut Serum Replacement is used to support the growth of undifferentiated embryonic stem (ES) and human induced pluripotent stem (iPSC) cells in culture.6 d' G: g; F6 C0 y. F3 c
One of the most important parameters surrounding ES cell culture is the maintenance of ES cells in an undifferentiated state. Until now, FBS was the only option for nutritive supplementation of ES and iPS cell media. Unfortunately, FBS also contains factors which can promote differentiation of ES cells. Thus, each lot must be screened prior to use. In addition, each lot of FBS is different from the last one, or the next one, so screening is a continuous task.
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Since FBS screening is laborious and costly, once a suitable lot has been identified, a sufficient quantity is purchased and stored to meet laboratory needs. This ties up both laboratory funds and storage space. Additionally, there are issues surrounding hemoglobin and endotoxin levels in FBS. All of the drawbacks of serum supplemented ES cell culture, including heat-inactivation of FBS, can be alleviated with the use of KnockOut™ SR, Serum Replacement for Embryonic Stem Cells and Human Induced Pluripotent Stem Cells. % Z3 c% y/ O/ y( h
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KnockOut™ SR is a defined formulation that will provide you with consistent growth conditions for your ES and iPS cell cultures. ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least. The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture.& S/ a/ ]9 ~: {, m% y% v
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http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Culture/Stem-Cell-Research/Embryonic-Stem-Cells/ESC-Stem-Cell-Culture-Media-Reagents/knockout-serum-replacement.html
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conanthird

leisong12 发表于 2011-5-17 22:37 # e: s3 }( q$ I5 v8 A/ G
What is KSR, would you explain it in detail.

3 U  x/ n6 j+ C0 L% l7 u, n5 f
KnockOut Serum Replacement is used to support the growth of undifferentiated embryonic stem (ES) and human induced pluripotent stem (iPSC) cells in culture.
) s5 {4 `. a9 o$ i' w3 V$ ROne of the most important parameters surrounding ES cell culture is the maintenance of ES cells in an undifferentiated state. Until now, FBS was the only option for nutritive supplementation of ES and iPS cell media. Unfortunately, FBS also contains factors which can promote differentiation of ES cells. Thus, each lot must be screened prior to use. In addition, each lot of FBS is different from the last one, or the next one, so screening is a continuous task.
' a- K1 E- r9 d1 g$ T/ G/ z8 E' ?. h* s; I
Since FBS screening is laborious and costly, once a suitable lot has been identified, a sufficient quantity is purchased and stored to meet laboratory needs. This ties up both laboratory funds and storage space. Additionally, there are issues surrounding hemoglobin and endotoxin levels in FBS. All of the drawbacks of serum supplemented ES cell culture, including heat-inactivation of FBS, can be alleviated with the use of KnockOut™ SR, Serum Replacement for Embryonic Stem Cells and Human Induced Pluripotent Stem Cells. 1 g0 C" r$ D1 x2 d4 ^: ]

# |  e; j$ Q; L1 oKnockOut™ SR is a defined formulation that will provide you with consistent growth conditions for your ES and iPS cell cultures. ES and iPS cells grown in KnockOut™ SR-supplemented media are substantially less differentiated than those grown in FBS-supplemented media, and germline transmission is not compromised in the least. The performance of KnockOut™ SR is further enhanced when used in conjunction with KnockOut™ DMEM, a special basal medium designed specifically for ES and iPS cell culture.  ^3 M; Z( D4 A
3 y, k# t' P6 a, L- d  \
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Culture/Stem-Cell-Research/Embryonic-Stem-Cells/ESC-Stem-Cell-Culture-Media-Reagents/knockout-serum-replacement.html
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