急需人软骨原代培养方法

jlzheng1224

valentiboa 发表于 2012-11-13 01:45
; Y# c; L) y. f回复 骨道边 的帖子- y) o6 F% ?, t: A: h

8 }" j0 t6 ^, z0 a( B这是我用来消化培养软骨细胞的方法，屡试不爽

% z- w4 \5 u! dThe tissues were transferred into a centrifuge tube and pre-digested in 10mL(per gram) alpha MEM(100U/mL penicillin, 100mg/mL streptomycin, 10% FBS) containing 25,000 IU/ml trypsin(1%), , for 15min in room temperature.; J: A2 P  k# c5 ~% W/ \' [5 z

! T/ Z, U/ I: a- T1 l您好，这一步同时加入胰酶和FBS，FBS不会抑制胰酶活性吗？？