Xeno-free hESCs

dongxin

Xeno-free hESCs  是什么意思？

huangcong1988

韩国科研人员克隆出一头拥有人类免疫基因的转基因迷你猪“Xeno”，已经成功存活90天，报道称，如果将拥有“FasL基因”的猪的器官移植给人类，人体排斥反应会大大减少。
: X' N/ q- w/ T3 k- D0 F上面是百度到的，那个xeno难道有这种解释？有原文没？没有语境不好翻译

huangcong1988

那个hESC是人类胚胎干细胞

scienese

回复 dongxin 的帖子
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! d5 \" I* _8 H4 ^俺的理解: 在分离和长期培养人ESC和IPS cell 的过程中,消除动物材料，比如动物血清 。这样一来胚胎干细胞在临床应用方面 就会更加安全， 会降低免疫排斥反应 ，消除动物材料所带来的 非人类的病原体。
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) X2 m! i1 |# ?具体参考：uman embryonic stem cells (hESCs) hold great promise for future clinical cell therapies in the fields of, for example, diabetes, cardiac infarction, and neurodegenerative diseases, because of their unique potential to differentiate into all cell types found in the human body. In addition, it has recently been revealed that hESCs and their differentiated derivatives are less susceptible to immune rejection than adult cells [1], which is encouraging news for future clinical application. However, the majority of hESC lines available to date have been directly or indirectly exposed to animal material during their derivation and/or propagation in vitro. Transplanting xeno-contaminated hESCs to patients will increase the risk of graft rejection [2] and transfer of nonhuman pathogens, suggesting that the existing hESC lines are unsuitable for clinical applications. Closer inspection of previous attempts to establish xeno-free hESC lines reveals, in each case, that not all animal products had been replaced. For example, several recent hESC derivation reports [3, [4]–5] used Knock-Out serum replacement (SR), which contains animal protein and is a source of nonhuman sialic acid [2]. Although Richards et al. [6] used human serum (HS) and human feeder cells, and Ludwig et al. [7] recently presented the derivation of two human ES cell lines (WA15 and WA16) in a chemically defined cell culture system, they both used immunosurgery to isolate the inner cell mass (ICM). Immunosurgery is a method commonly used to remove the outer trophectoderm epithelial cell layer from the blastocyst by incubation in polyclonal rabbit anti-human whole-serum antibodies and guinea pig complement. Consequently, although these culture systems may be free of animal products, the derivation procedure is not, and therefore, these hESC lines should be considered potentially xeno-contaminated.

张也行

同意楼上的回答，应该是在无血清，无饲养层培养的hESC吧。现在hESC的培养正朝着已知成分的培养基方向发展，因为血清中含有未知成分的物质，可能会引起免疫排除，而饲养层一般用小鼠的MEF，如果ES细胞不纯，也可能引起免疫排斥。

dongxin

谢谢！我想应该是 无异源性人胚胎干细胞!

xxh83121

就是无其他非人源性的培养基培养的干细胞，Contains components only of human origin (xeno-free)

xxh83121

这是stem search公司的产品介绍，看一下

dongxin

回复 xxh83121 的帖子4 |$ T- [% Y+ G

# f- |) F4 Y& H- B6 w谢谢！