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本帖最后由 细胞海洋 于 2011-6-11 14:46 编辑 , c V) A2 d) |
. D _7 @9 a1 ]8 {8 n. F& I" vPCR Cloning Protocols& c$ s& ?" U* c$ ], R- Z
Second Edition
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Contents1 p+ B( D. R |( ?! q9 J2 F
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Preface .............................................................................................................v
) `+ \# O4 o, H& f6 l( GContributors .....................................................................................................xi, a* N$ o$ Y) [# b+ \" d
PART I. PERFORMING AND OPTIMIZING PCR) e3 V) l. z7 X; b' E+ m+ d
1 Polymerase Chain Reaction: Basic Principles and Routine Practice P- ^* N4 z9 T# W
Lori A. Kolmodin and David E. Birch .................................................. 35 J2 t' N+ @ [, k
2 Computer Programs for PCR Primer Design and Analysis
+ _2 Z) T% P, ?1 e2 w& qBing-Yuan Chen, Harry W. Janes, and Steve Chen ........................ 19
3 c, ]* Q |6 ]$ I3 Single-Step PCR Optimization
/ g2 o/ {* ?% P0 g2 `Using Touchdown and Stepdown PCR Programming' ?! S% t6 S" O# u) R
Kenneth H. Roux .................................................................................. 31
) |5 W4 P V% k8 p& d, d5 w4 XL PCR Amplification of Long Targets from Genomic DNA
% ^& j7 s' K8 q7 R- a6 o$ i3 U YLori A. Kolmodin .................................................................................. 37
+ ~' B1 X$ B3 ]5 Coupled One-Step Reverse Transcription and Polymerase Chain
5 z8 t1 Z* g5 B uReaction Procedure for Cloning Large cDNA Fragments7 r" H) l$ R1 j* e0 d8 _0 |% I
Jyrki T. Aatsinki ................................................................................... 53" L- T1 O" q0 C' O+ M
6 Long Distance Reverse-Transcription PCR+ W! v& h) O, X! K
Volker Thiel, Jens Herold, and Stuart G. Siddell ............................. 59
# u! Z0 ]8 K- F1 r: m' f Y7 Increasing PCR Sensitivity for Amplification
) j p" \0 v' u' o0 mfrom Paraffin-Embedded Tissues- D# t# y4 e# }5 e; [% K @
Abebe Akalu and Juergen K. V. Reichardt ....................................... 676 _! o/ P% \8 i
8 GC-Rich Template Amplification by Inverse PCR:* _% `1 a; Z2 T
DNA Polymerase and Solvent Effects- X3 ^; l% @" f8 m
Alain Moreau, Da Shen Wang, Steve Forget, Colette Duez,
( I3 R8 ~( b* u5 Band Jean Dusart............................................................................... 75
% D8 L2 K* l2 w( I$ C9 PCR Procedure for the Isolation of Trinucleotide Repeats
4 t4 r! X9 {$ V7 j6 yTeruaki Tozaki ...................................................................................... 81
, v% i3 b) l6 t, O4 a10 Methylation-Specific PCR
7 d" h9 P- q/ v; j7 `Haruhiko Ohashi .................................................................................. 91
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11 Direct Cloning of Full-Length Cell Differentially Expressed Genes
! L9 n, t5 b3 T6 mby Multiple Rounds of Subtractive Hybridization% y9 t) _! w& ?7 G# D) J
Based on Long-Distance PCR and Magnetic Beads+ J8 M* A9 D+ _: W: R* c
Xin Huang, Zhenglong Yuan, and Xuetao Cao ................................ 99
- _. u# T' W: d" Y. S' P' b- ePART II. CLONING PCR PRODUCTS
" F( k( E( ?9 V* A12 Cloning PCR Products: An Overview
. P# V. p6 |, r8 RBaotai Guo and Yuping Bi ................................................................ 111( p8 \" m5 }: E/ K+ j7 B
13 Using T4 DNA Polymerase to Generate Clonable PCR Products
- C4 T+ |: W: N$ }: J$ yKai Wang ............................................................................................. 121( b( i: @0 i7 P" Y
14 Enzyme-Free Cloning of PCR Products; t. O9 T6 ^+ m4 c0 W. K
and Fusion Protein Expression9 C$ Z4 D+ I, m9 X( c
Brett A. Neilan and Daniel Tillett ..................................................... 1253 I* S0 d; q# E. I
15 Directional Restriction Site-Free Insertion of PCR Products
+ I1 o s7 R' b9 w/ o9 I/ {2 dinto Vectors
. p9 w, J# n0 k; Y2 }* H8 H" b4 dGuo Jun Chen .................................................................................... 133
# `- ^9 j6 i0 \: ~1 j+ A9 J16 Autosticky PCR:
$ c0 e; { ~' d: ?0 B% bDirectional Cloning of PCR Products with Preformed 5' Overhangs
6 E: i/ }9 c4 E% Q# C/ IJózsef Gál and Miklós Kálmán......................................................... 1413 M5 y: q% _$ I5 m4 A' g0 z
17 A Rapid and Simple Procedure for Direct Cloning( U3 O" b" ]3 I; }
of PCR Products into Baculoviruses1 w; w& Q" h8 V
Tamara S. Gritsun, Michael V. Mikhailov,
1 a3 |$ F5 n. w) U% I* l4 g1 k2 Vand Ernest A. Gould ...................................................................... 153
3 [, h8 a+ V% Y/ PPART III. MUTAGENESIS AND RECOMBINATION
' E0 V/ e% C8 @5 ?( z0 I7 N18 PCR Approaches to DNA Mutagenesis and Recombination:5 p9 G+ `: F# a: q2 |/ z
An Overview: i: }0 G4 o L2 @
Binzhang Shen ................................................................................... 167. W% g6 G% t* y6 a
19 In-Frame Cloning of Synthetic Genes Using PCR Inserts
' y) k# d1 ]& P: \# QJames C. Pierce ................................................................................. 175
+ H; X( w7 H+ X$ l5 b) `8 O: a20 Megaprimer PCR# I* r1 B& [# ~. ]. A
Sailen Barik ........................................................................................ 189
& ~' Q4 x2 {( a' g; E# w21 PCR-Mediated Recombination:& W9 [9 l% M4 L8 T. \ a/ h u
A General Method Applied to Construct Chimeric Infectious
& h0 o) }$ h, W( y" CMolecular Clones
8 q/ j6 h& I* X2 r+ [# DGuowei Fang, Barbara Weiser, Aloise Visosky, Timothy Moran,
, _8 ~& q+ q& [9 ~$ A8 jand Harold Burger ......................................................................... 1971 B W% n( e3 U
22 PCR Method for Generating Multiple Mutations at Adjacent Sites
! }2 {: b: f) \9 e6 lJiri Adamec ......................................................................................... 207. O; |$ E+ u: n2 f" L
( S+ o5 @" e* U# o' z% ~: c5 z z; s23 A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing
0 J/ j3 s- h U' K: [( f7 l' CRepeat Expansions into CAG-Repeat Containing Genes
: E5 o0 I% G: I, F+ o6 \* ~Franco Laccone ................................................................................. 217" a5 n, r3 ?$ ~' j; O9 g* I2 H
24 PCR Screening in Signature-Tagged Mutagenesis of Essential Genes
( c3 m0 o2 k/ xDario E. Lehoux and Roger C. Levesque ....................................... 225
+ _8 C1 A3 @5 Z0 P' x25 Staggered Extension Process (StEP) In Vitro Recombination" K- w" A I2 u
Anna Marie Aguinaldo and Frances Arnold ................................... 235
( Y) B6 m c# K/ a" {, n. U4 ]26 Random Mutagenesis by Whole-Plasmid PCR Amplification
, T! X# ^% @7 ?1 I: EDonghak Kim and F. Peter Guengerich .......................................... 241
) D% u- {& D. hPART IV. CLONING UNKNOWN NEIGHBORING DNA4 ?- l1 w! [ }; e
27 PCR-Based Strategies to Clone Unknown DNA Regions
4 r7 b1 s8 a L! E3 X% jfrom Known Foreign Integrants: An Overview1 [: D8 m0 B5 U
Eric Ka-Wai Hui, Po-Ching Wang, and Szecheng J. Lo ................ 2491 D5 i9 N. B2 m2 A' n3 e
28 Long Distance Vectorette PCR (LDV PCR)
, C1 b: k4 _5 q4 @; b; UJames A. L. Fenton, Guy Pratt, and Gareth J. Morgan ................. 2750 z# @7 W0 Z* {8 l+ S3 d
29 Nonspecific, Nested Suppression PCR Method0 s( i8 B8 S* r, h `
for Isolation of Unknown Flanking DNA (“Cold-Start Method”)* X" f, v( N& a: f8 M
Michael Lardelli .................................................................................. 285 x. Q( @- }9 C/ O
30 Inverse PCR: cDNA Cloning3 w3 K0 C+ q$ U+ x4 {+ \
Sheng-He Huang ................................................................................ 2937 r1 W; `$ C$ d; p
31 Inverse PCR: Genomic DNA Cloning$ J' U2 F. \: [+ m8 ?) i
Ambrose Y. Jong, Anna T’ang, De-Pei Liu,
8 j, z( ^9 Z9 Q. n4 dand Sheng-He Huang .................................................................... 3017 y8 Z9 K1 r( N' J# o6 E
32 Gene Cloning and Expression Profiling by Rapid Amplification
/ l% d6 G6 I( [, P4 B( c" D/ ^of Gene Inserts with Universal Vector Primers
' F- y! u# \( |9 LSheng-He Huang, Hua-Yang Wu, and Ambrose Y. Jong .............. 309
) X' V0 }% _2 a* k33 The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions
d* h% H5 M/ Z1 E8 f% v: [by Inverse PCR! W4 H- E$ G' S# C, F$ C9 E
Vincent J. J. Martin and William W. Mohn ...................................... 315& k. S, {* J+ b& S
34 Rapid Amplification of Genomic DNA Sequences Tagged
7 j5 P9 N0 ]: [1 Q5 N# K# ^+ `by Insertional Mutagenesis6 j/ E# }' j4 l" G) f
Martina Celerin and Kristin T. Chun ................................................ 3258 D" N4 W, j7 s1 d; @
35 Isolation of Large Terminal Sequences of BAC Inserts Based }0 v. p5 e+ e& N
on Double-Restriction-Enzyme Digestion Followed
, ^6 @$ T s: L9 cby Anchored PCR
% @. o: U _4 Z4 c7 @Zhong-Nan Yang and T. Erik Mirkov ............................................... 337
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2 g$ x6 m" J' n+ ]36 A “Step Down” PCR-Based Technique for Walking3 C- h, k6 t$ u, q
Into and the Subsequent Direct Sequence Analysis6 I" R7 P0 W1 R. N
of Flanking Genomic DNA! g2 I& l$ x3 A; T
Ziguo Zhang and Sarah Jane Gurr .................................................. 343
" d5 C9 b- ^! Z* s9 N' ], EPART V. LIBRARY CONSTRUCTION AND SCREENING
8 ?7 L" z! h( b. X5 r37 Use of PCR in Library Screening: An Overview
3 U% S$ P; x* t- S' _# p/ x8 o3 mJinbao Zhu .......................................................................................... 353
0 f2 W8 f8 i0 e; s, w38 Cloning of Homologous Genes by Gene-Capture PCR1 ]- I! S* x: T x8 P) z( \
Renato Mastrangeli and Silvia Donini ............................................. 359# d7 S3 Q" C& B$ y6 `7 j
39 Rapid and Nonradioactive Screening of Recombinant Libraries by PCR. N% N, J7 F+ ^# G+ s( _
Michael W. King ................................................................................. 3776 g0 C! C2 b% z+ {2 w5 B
40 Rapid cDNA Cloning by PCR Screening (RC-PCR)$ ^( E" L; C9 M
Toru Takumi ....................................................................................... 385/ \7 L; E+ J5 w, K. T, Z3 V
41 Generation and PCR Screening of Bacteriophage λ Sublibraries
4 s% s7 s- @# z7 V* UEnriched for Rare Clones (the “Sublibrary Method”)
8 k: `- |, f$ Q4 IMichael Lardelli .................................................................................. 391
# _1 A4 k! N/ r7 ]" g# ^42 PCR-Based Screening for Bacterial Artificial Chromosome Libraries
. n4 A& N7 m" kYuji Yasukochi ................................................................................... 401
- k( M" M" e/ W' I0 C43 A 384-Well Microtiter-Plate-Based Template Preparation
, M5 {, w0 U; A8 e# l0 a9 u2 uand Sequencing Method
' N8 X) Q( P& t. a7 w0 bLei He and Kai Wang ......................................................................... 4119 p" H- ?* v- q) c) N
44 A Microtiter-Plate-Based High Throughput PCR Product" K; ~/ w7 t2 N2 D& f0 s
Purification Method8 D7 s- J; q& b; [
Ryan Smith and Kai Wang ................................................................ 417
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1 a& R) i% u$ L1 \5 j' z. {1 }3 A[hide][/hide]+ @; ]4 Z$ ^* ^' u: j( ^9 i" k
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发表于 2011-6-11 11:43
发表于 2011-6-11 14:46
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