|
 
- 积分
- 404
- 威望
- 404
- 包包
- 9
|
本帖最后由 细胞海洋 于 2011-6-11 14:46 编辑 ' l, Z z( ]( P4 L
0 N3 K* L* ?/ Y. j; \PCR Cloning Protocols
: x" u8 @+ r( g3 |Second Edition+ u' P# K' z+ G1 E( d
7 q3 C* Y1 G3 q- V* F
& _! n3 _' A+ s3 U: E2 yContents
: W4 \+ D7 t7 _4 v4 U5 rvii4 x$ k9 C" E& l. m" G! @) r6 M+ T- [
Preface .............................................................................................................v
" [8 q' J0 H4 ~1 o& cContributors .....................................................................................................xi
: h# ], H/ k* t* j! TPART I. PERFORMING AND OPTIMIZING PCR0 d/ u* v1 F2 x: @
1 Polymerase Chain Reaction: Basic Principles and Routine Practice
. C" h# t, Y( z* A. Y( K0 k/ v' K; ULori A. Kolmodin and David E. Birch .................................................. 3& _7 I$ H6 L. \$ l' r% Z |" p9 z
2 Computer Programs for PCR Primer Design and Analysis2 W. _! p- v8 O
Bing-Yuan Chen, Harry W. Janes, and Steve Chen ........................ 19
' b/ C3 Q* M, P: n, P+ X3 Single-Step PCR Optimization! ^# a% g2 x0 n. x$ s
Using Touchdown and Stepdown PCR Programming* u# `) w, G- q+ ]
Kenneth H. Roux .................................................................................. 31 l+ m ?6 f& j
4 XL PCR Amplification of Long Targets from Genomic DNA
) D/ F+ k w7 s) k- F" ^Lori A. Kolmodin .................................................................................. 37
5 }3 o8 z" [1 n5 Coupled One-Step Reverse Transcription and Polymerase Chain
) R y' h' D8 d/ Y' z# ZReaction Procedure for Cloning Large cDNA Fragments
6 V8 @& ^* A$ |5 t& p$ `4 kJyrki T. Aatsinki ................................................................................... 53
% }) K, O% _6 o* m0 J7 \* N" m6 Long Distance Reverse-Transcription PCR. F" d# O5 v/ z, `2 k0 P2 U5 S/ B* M( @
Volker Thiel, Jens Herold, and Stuart G. Siddell ............................. 59
$ U" R9 [/ f: i! _+ l) G7 Increasing PCR Sensitivity for Amplification
! L. f0 Q Z5 q8 o' N& e7 ifrom Paraffin-Embedded Tissues
( I1 j, y3 v/ h8 ]3 B" ^, {Abebe Akalu and Juergen K. V. Reichardt ....................................... 67. }$ |8 ]7 g+ ]" Q: X: D- Z
8 GC-Rich Template Amplification by Inverse PCR:/ Z/ E# M ^' U; A7 P7 o4 R6 U
DNA Polymerase and Solvent Effects
' m7 E O/ e% G' ^Alain Moreau, Da Shen Wang, Steve Forget, Colette Duez,7 A, J3 @! w* ^, W/ O
and Jean Dusart............................................................................... 75
) L* w- W, w4 O9 PCR Procedure for the Isolation of Trinucleotide Repeats- Y& F# X7 N. i
Teruaki Tozaki ...................................................................................... 81
$ F) [: i4 T5 @- H6 M) w! Z0 k2 T10 Methylation-Specific PCR
$ O9 J( D. B+ D9 h" vHaruhiko Ohashi .................................................................................. 91# `% z) E; x7 Y/ x. L! s8 J7 f
! u; R+ P5 g: m1 v7 f
11 Direct Cloning of Full-Length Cell Differentially Expressed Genes9 _, A: w7 _6 o, p' c9 \7 @" t
by Multiple Rounds of Subtractive Hybridization
4 f+ J6 P, J, i- I. V: xBased on Long-Distance PCR and Magnetic Beads
' U4 U" W+ v' h1 B5 B* JXin Huang, Zhenglong Yuan, and Xuetao Cao ................................ 99- L& ^8 m. G+ o9 z a9 }
PART II. CLONING PCR PRODUCTS
. P& K$ h+ a [1 \( ?12 Cloning PCR Products: An Overview5 i- @& t' c4 }) U; R( f" Q$ m
Baotai Guo and Yuping Bi ................................................................ 111
4 [9 z7 b" O1 } p% @5 f13 Using T4 DNA Polymerase to Generate Clonable PCR Products' a/ K' U/ x3 M) B+ P8 ^# X
Kai Wang ............................................................................................. 121
* w! O; o$ G0 }+ j2 Y14 Enzyme-Free Cloning of PCR Products
3 |5 g' R- ~3 T! fand Fusion Protein Expression
& u$ z9 D: d% X. n7 J# t+ mBrett A. Neilan and Daniel Tillett ..................................................... 125
7 ~0 b, ?! p( V% P: u( b" j* B E$ X15 Directional Restriction Site-Free Insertion of PCR Products
2 p5 O! p1 k5 \& w/ G" `into Vectors
. r9 i5 s$ p; F1 y2 qGuo Jun Chen .................................................................................... 133; W3 W) _" J5 n& L6 o
16 Autosticky PCR:1 [2 `$ [ T2 Z+ Y( J$ P* X, X8 V
Directional Cloning of PCR Products with Preformed 5' Overhangs G- ?! \& q# ~1 m$ u* z( ~3 s
József Gál and Miklós Kálmán......................................................... 141- o) [/ U5 Z0 m* _
17 A Rapid and Simple Procedure for Direct Cloning
/ f- N% f2 g4 b$ O7 x2 ?of PCR Products into Baculoviruses
: V% q3 A6 ^8 s! uTamara S. Gritsun, Michael V. Mikhailov,
" Q6 R7 J& f" p3 Hand Ernest A. Gould ...................................................................... 1534 a6 Y- H, |: c
PART III. MUTAGENESIS AND RECOMBINATION8 x. e S3 D; N' `
18 PCR Approaches to DNA Mutagenesis and Recombination:
, z V2 H3 V6 [' p9 k& @An Overview
, l& v9 R# u, c7 CBinzhang Shen ................................................................................... 167
% }! Z! \, k. P$ R9 Y19 In-Frame Cloning of Synthetic Genes Using PCR Inserts0 i( h3 w( E, Z5 e% z+ B
James C. Pierce ................................................................................. 1755 T: F" V& m. R( M i% a$ F
20 Megaprimer PCR
7 G! V0 L; Q7 \! ASailen Barik ........................................................................................ 189/ T! E) p' _* [/ r# {; G& E( |2 W
21 PCR-Mediated Recombination:2 C+ p7 I. R1 M; E, F) B1 [1 O% y
A General Method Applied to Construct Chimeric Infectious
& H/ i6 u, [2 N/ mMolecular Clones) l2 K! Y& w- X9 E* o
Guowei Fang, Barbara Weiser, Aloise Visosky, Timothy Moran,
9 k, I" n4 l* D% G5 Tand Harold Burger ......................................................................... 197
: u7 A5 L) X" Y& {$ s- d" v7 l9 N22 PCR Method for Generating Multiple Mutations at Adjacent Sites
% B, k3 Y0 `' h1 F/ TJiri Adamec ......................................................................................... 207
' `7 v5 c; |' y9 I
; Z! s$ A1 v4 G g23 A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing
) o" C" x1 V: a- j6 W1 Y5 F& D, [: nRepeat Expansions into CAG-Repeat Containing Genes
) V+ @2 r3 H2 J9 uFranco Laccone ................................................................................. 217$ c2 P, v0 n3 L# Z4 z1 a
24 PCR Screening in Signature-Tagged Mutagenesis of Essential Genes
, a+ ?( K% E2 [Dario E. Lehoux and Roger C. Levesque ....................................... 225
: [/ @! l' `1 x N$ V5 E2 h. p25 Staggered Extension Process (StEP) In Vitro Recombination3 D J- ^& o; A, b
Anna Marie Aguinaldo and Frances Arnold ................................... 235
3 k8 k8 e0 k( x* _26 Random Mutagenesis by Whole-Plasmid PCR Amplification
( ^8 D% r6 B5 g* u1 [' I0 gDonghak Kim and F. Peter Guengerich .......................................... 241
" i c3 |/ @' f+ x. O1 [+ \- JPART IV. CLONING UNKNOWN NEIGHBORING DNA+ X1 C: k1 A* S% P1 f% J, Z
27 PCR-Based Strategies to Clone Unknown DNA Regions
# q6 J2 R; A0 `3 J+ qfrom Known Foreign Integrants: An Overview4 Y7 h' S; Z- i( j5 x
Eric Ka-Wai Hui, Po-Ching Wang, and Szecheng J. Lo ................ 249* u& j; _, f' f2 q5 S
28 Long Distance Vectorette PCR (LDV PCR)
% v4 ~# A& }/ QJames A. L. Fenton, Guy Pratt, and Gareth J. Morgan ................. 275! L! X4 T' J- z
29 Nonspecific, Nested Suppression PCR Method1 }8 T3 {" F ?9 s+ F: v: F
for Isolation of Unknown Flanking DNA (“Cold-Start Method”)9 O3 C6 T1 }* U& x/ l3 t3 ^
Michael Lardelli .................................................................................. 285
8 ?' i$ d( n: _3 q30 Inverse PCR: cDNA Cloning
. B3 J# x8 s/ T( x P( `& TSheng-He Huang ................................................................................ 293
9 i3 \2 P/ z3 c9 B& ]31 Inverse PCR: Genomic DNA Cloning* ?. P! w& ?/ J; x( C* ^; \3 Y* N3 K
Ambrose Y. Jong, Anna T’ang, De-Pei Liu," v0 E) y4 `8 M" j$ ~0 K
and Sheng-He Huang .................................................................... 301
8 H: t& o3 v" q9 x- Z& p32 Gene Cloning and Expression Profiling by Rapid Amplification
/ d. J, _1 C$ Q* c" j4 ?- Tof Gene Inserts with Universal Vector Primers
/ U) @/ T! M/ V& ^' G) TSheng-He Huang, Hua-Yang Wu, and Ambrose Y. Jong .............. 309 e- G& d0 g9 }+ _7 i) D4 J6 a# A
33 The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions
4 V3 C# }) d1 Q* u3 m$ |by Inverse PCR4 @$ @& `8 y- }/ f
Vincent J. J. Martin and William W. Mohn ...................................... 3156 e7 l) t- p3 q
34 Rapid Amplification of Genomic DNA Sequences Tagged
8 I+ X- B0 P; U1 I# M+ Hby Insertional Mutagenesis
* J- ~2 t& ?/ i, ?Martina Celerin and Kristin T. Chun ................................................ 325' V) {' |3 Z2 U* P5 ~9 b/ |1 G
35 Isolation of Large Terminal Sequences of BAC Inserts Based
3 ]9 \8 Q! {! H' {/ D3 `. yon Double-Restriction-Enzyme Digestion Followed
" p- R6 v8 Z! B0 F* f8 j0 q8 W( _by Anchored PCR! I1 ?# b- E J N+ Y' D
Zhong-Nan Yang and T. Erik Mirkov ............................................... 3379 b- `/ P+ z; K; O+ l5 Y
5 O2 D& `$ V+ U) K36 A “Step Down” PCR-Based Technique for Walking
! K U: C1 }" W* F6 wInto and the Subsequent Direct Sequence Analysis
5 u7 o& h' l( Y$ \" Z6 `3 m0 G6 d9 T' Mof Flanking Genomic DNA- |& |( W/ j, c, e$ a- c* `
Ziguo Zhang and Sarah Jane Gurr .................................................. 3430 H( j4 B: g a- F+ q
PART V. LIBRARY CONSTRUCTION AND SCREENING
" A( W1 K" B+ S6 l37 Use of PCR in Library Screening: An Overview! T1 q- [3 S ^6 ]; G" X
Jinbao Zhu .......................................................................................... 353
- M+ H$ B) o/ K" C+ J38 Cloning of Homologous Genes by Gene-Capture PCR
6 ]+ j4 P" {! ~' m _: \4 S8 W2 IRenato Mastrangeli and Silvia Donini ............................................. 359
! }2 v7 q4 R5 W39 Rapid and Nonradioactive Screening of Recombinant Libraries by PCR
2 k" p$ v& Y# g/ H2 q, D5 tMichael W. King ................................................................................. 377
; _% Y: F( H5 j6 j4 V40 Rapid cDNA Cloning by PCR Screening (RC-PCR) R5 q' ~. b. G8 u' F2 ]& U
Toru Takumi ....................................................................................... 385% [: v# i; w* H' T, m
41 Generation and PCR Screening of Bacteriophage λ Sublibraries" G% ?; h5 [0 I* S- a
Enriched for Rare Clones (the “Sublibrary Method”): z4 O7 r9 Z! @
Michael Lardelli .................................................................................. 391
5 y* Y* c; _, Z$ b0 O1 k42 PCR-Based Screening for Bacterial Artificial Chromosome Libraries
, x& H: j6 c& D3 \6 }( sYuji Yasukochi ................................................................................... 4010 [8 h% | F# I, E5 _
43 A 384-Well Microtiter-Plate-Based Template Preparation1 W* E: h0 b. o4 Q2 f# {/ D' l
and Sequencing Method
& w* ?& x, Y5 G9 [8 [2 QLei He and Kai Wang ......................................................................... 411
m: o+ j' v) o9 B W! W2 U0 D8 g44 A Microtiter-Plate-Based High Throughput PCR Product8 z8 w6 ?# X+ x2 ]% ]; ^ P
Purification Method6 V1 s% z$ J% T0 _
Ryan Smith and Kai Wang ................................................................ 417 5 S) _& M" r( O' V
0 w8 @" H8 Z9 m4 l
[hide][/hide]
% f, c2 G6 G5 `! B1 {1 F6 c7 O |
附件: 你需要登录才可以下载或查看附件。没有帐号？注册
|
发表于 2011-6-11 11:43
发表于 2011-6-11 14:46
