|
 
- 积分
- 404
- 威望
- 404
- 包包
- 9
|
本帖最后由 细胞海洋 于 2011-6-11 14:46 编辑
* h2 x2 s3 V6 r
6 c9 V8 G% o7 Q4 T/ R& [PCR Cloning Protocols5 U3 y+ }7 I! d G6 A
Second Edition
/ F) ?$ _+ R+ D+ P& y o/ ?- x! S9 a5 y x4 K
5 K) X) X. R$ ^/ `9 v
Contents
' r% P7 D1 S! I3 Gvii0 b+ C- J" B, C8 S3 x: o5 M& u' E
Preface .............................................................................................................v: W+ I( N) Z' l4 H) D; h, B. R
Contributors .....................................................................................................xi# T3 N& C4 t2 v+ E. J7 U- ]
PART I. PERFORMING AND OPTIMIZING PCR3 o# _5 j. Z3 [. d0 ~8 |
1 Polymerase Chain Reaction: Basic Principles and Routine Practice) r) L+ B4 l J1 P; f8 U
Lori A. Kolmodin and David E. Birch .................................................. 3
9 M) ]0 E0 z; W) E2 }# v2 Computer Programs for PCR Primer Design and Analysis
' R" H' R& C: A& `$ \3 m" ^2 gBing-Yuan Chen, Harry W. Janes, and Steve Chen ........................ 19
" r+ f8 [4 U1 }! j, W3 V! w4 M3 Single-Step PCR Optimization+ [ a# _' z! m: @! T
Using Touchdown and Stepdown PCR Programming) G8 u0 Q: g5 O. i3 k7 D
Kenneth H. Roux .................................................................................. 31
+ V) u+ O; r; K$ A/ P5 c% \* I* C4 XL PCR Amplification of Long Targets from Genomic DNA
% V4 h/ p, |. e; s% c! g! SLori A. Kolmodin .................................................................................. 37
; Y& U9 K+ [- u5 l1 d: e5 Coupled One-Step Reverse Transcription and Polymerase Chain
" @5 w0 N; Z4 p4 B/ X: B# aReaction Procedure for Cloning Large cDNA Fragments5 M0 X! w% ~! k- r' T
Jyrki T. Aatsinki ................................................................................... 530 V. Q; }- R, {
6 Long Distance Reverse-Transcription PCR: U! I, P$ C# x. B
Volker Thiel, Jens Herold, and Stuart G. Siddell ............................. 59* v _9 f; c' D$ h, I/ b
7 Increasing PCR Sensitivity for Amplification' Q0 {7 E' \. o: C# Q7 p' ]
from Paraffin-Embedded Tissues
9 O/ v( h1 g W5 B7 e- a. [Abebe Akalu and Juergen K. V. Reichardt ....................................... 67
/ ~5 u3 N) _; w( b' s% Z8 GC-Rich Template Amplification by Inverse PCR:2 j1 N) s+ f! W7 G }6 @: I/ e M
DNA Polymerase and Solvent Effects
# W4 U: [% w5 w0 Z8 {1 p) h+ nAlain Moreau, Da Shen Wang, Steve Forget, Colette Duez,) x! K( {7 \+ Z, s: k1 s7 ^
and Jean Dusart............................................................................... 75- b! b4 U1 z7 { D" O- q
9 PCR Procedure for the Isolation of Trinucleotide Repeats% X) f! i, s2 j0 _- j
Teruaki Tozaki ...................................................................................... 81! L! D% R& L# E2 L; n4 Q% n- y
10 Methylation-Specific PCR
; Z" O( a( C" v1 @Haruhiko Ohashi .................................................................................. 91
+ s8 z4 E7 ~" o, L+ q J0 D3 k
" A8 m$ j: o: @8 m+ k* T11 Direct Cloning of Full-Length Cell Differentially Expressed Genes
; _ y$ G/ y& b/ gby Multiple Rounds of Subtractive Hybridization
* u9 Y7 C9 }: _; l3 UBased on Long-Distance PCR and Magnetic Beads: C" f' T6 O6 ]" s7 P! B
Xin Huang, Zhenglong Yuan, and Xuetao Cao ................................ 99
$ J/ d" i9 _7 h: V0 q3 u$ xPART II. CLONING PCR PRODUCTS# {2 F( x8 {$ ~0 K8 K2 P. d
12 Cloning PCR Products: An Overview) Y0 O8 @3 x; e- L& m2 N. ]
Baotai Guo and Yuping Bi ................................................................ 111
2 | ]! p, I. Q/ `13 Using T4 DNA Polymerase to Generate Clonable PCR Products4 X1 N( o$ |+ a/ ?! M9 @
Kai Wang ............................................................................................. 121
% {) T3 z* E5 l! h r14 Enzyme-Free Cloning of PCR Products$ V0 O! |: P }6 B" Q+ H1 d2 j7 S
and Fusion Protein Expression
! e( F4 q% w1 `! w! i [5 eBrett A. Neilan and Daniel Tillett ..................................................... 125
2 U8 U0 y" }7 d- w15 Directional Restriction Site-Free Insertion of PCR Products
% }! s7 f- @$ l' {into Vectors
y/ ]" r8 I$ F! W; l6 e7 @Guo Jun Chen .................................................................................... 133; y0 s; G/ |3 ~" T. Z; V
16 Autosticky PCR:
' P0 q. `, b' ]; M- xDirectional Cloning of PCR Products with Preformed 5' Overhangs: n3 Y4 e7 h/ }. Q
József Gál and Miklós Kálmán......................................................... 141- G7 B4 {' c" }3 J* R9 Y
17 A Rapid and Simple Procedure for Direct Cloning
+ d* n2 \1 \3 {7 V! Z$ o1 ^& Vof PCR Products into Baculoviruses" T5 u$ J! |% w! {
Tamara S. Gritsun, Michael V. Mikhailov,! I% L; A/ G0 y8 e$ J, H3 j
and Ernest A. Gould ...................................................................... 153
( E0 h7 J0 G W" y# R' C p4 @PART III. MUTAGENESIS AND RECOMBINATION
6 o7 F% Z) V- Z$ H8 T18 PCR Approaches to DNA Mutagenesis and Recombination:
# g& q. ?1 u- S9 }3 E( |4 [An Overview
" j( B9 i+ G( o6 L/ p: W" z6 Y" m. qBinzhang Shen ................................................................................... 167$ T+ ^9 f1 ^; ~1 d
19 In-Frame Cloning of Synthetic Genes Using PCR Inserts) y9 H Z3 l: X$ n. L- N, M" D7 ^
James C. Pierce ................................................................................. 175
' r3 `# y! N* q$ B; y0 I20 Megaprimer PCR
7 S6 n9 H9 d4 pSailen Barik ........................................................................................ 1895 ?- y, p( E8 X) L
21 PCR-Mediated Recombination:' v- b) l3 F+ [8 A# Y, h: P8 O
A General Method Applied to Construct Chimeric Infectious; \2 D( Z8 c5 S: u# `, Y2 e
Molecular Clones
9 {0 K. n+ S% _$ Y( g2 |7 ]Guowei Fang, Barbara Weiser, Aloise Visosky, Timothy Moran,' D ^0 p7 |- T- v. n& W( i; u
and Harold Burger ......................................................................... 197, W, x% ^% x9 @* K" z+ u
22 PCR Method for Generating Multiple Mutations at Adjacent Sites8 N9 u& b1 T) o& N+ c
Jiri Adamec ......................................................................................... 207
9 `9 x5 d; n* @7 j, c/ |, e/ h/ E2 [' y C4 K
23 A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing3 N( p8 f; D; g- D/ z. N
Repeat Expansions into CAG-Repeat Containing Genes
! G, t9 ]9 s7 F) S, M& [* W2 M5 [Franco Laccone ................................................................................. 217
; g( S9 ^; H/ V+ j24 PCR Screening in Signature-Tagged Mutagenesis of Essential Genes0 P# m8 H5 i/ y. f6 s
Dario E. Lehoux and Roger C. Levesque ....................................... 225/ U6 q4 N5 X+ {- w
25 Staggered Extension Process (StEP) In Vitro Recombination
4 Y: Z% I( ^# e. Q; {3 s* }Anna Marie Aguinaldo and Frances Arnold ................................... 235# @; Q J/ T' d2 v! K, [
26 Random Mutagenesis by Whole-Plasmid PCR Amplification
5 T) T% y. r9 H& \8 U5 cDonghak Kim and F. Peter Guengerich .......................................... 241
" I( K2 c5 m- bPART IV. CLONING UNKNOWN NEIGHBORING DNA* ]: `! N* m; g8 k6 e1 v
27 PCR-Based Strategies to Clone Unknown DNA Regions7 l: e- i9 v- C3 G
from Known Foreign Integrants: An Overview7 R% ?) s7 q5 z5 q$ v' j/ h* j
Eric Ka-Wai Hui, Po-Ching Wang, and Szecheng J. Lo ................ 249" E2 Z0 e4 E( q n$ h% |
28 Long Distance Vectorette PCR (LDV PCR)
, [9 }9 \, R, Y( G% _% n1 MJames A. L. Fenton, Guy Pratt, and Gareth J. Morgan ................. 275* D' l; r; C; q1 @
29 Nonspecific, Nested Suppression PCR Method
8 {/ `* a/ h3 Y- u0 N8 Hfor Isolation of Unknown Flanking DNA (“Cold-Start Method”)% g& L! l$ | A0 o
Michael Lardelli .................................................................................. 285
/ k( I$ T/ F! ]" Y/ e6 \30 Inverse PCR: cDNA Cloning
* W4 x3 y* R2 _+ C: Q, o n! y1 JSheng-He Huang ................................................................................ 293
* D7 y8 j" b+ q31 Inverse PCR: Genomic DNA Cloning2 ]& i9 h; ]% |( e& ?. e2 w R: {
Ambrose Y. Jong, Anna T’ang, De-Pei Liu,& O& T" R p" V
and Sheng-He Huang .................................................................... 301
0 H. M& b; u* P32 Gene Cloning and Expression Profiling by Rapid Amplification
. S: g$ C; x6 e* i5 ?of Gene Inserts with Universal Vector Primers
: W# N* d/ y2 c* Y/ q5 t1 `Sheng-He Huang, Hua-Yang Wu, and Ambrose Y. Jong .............. 309
$ n3 _* N7 p/ ]4 h& n+ {4 g33 The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions
c2 k0 N* c9 N: |by Inverse PCR. g& C* D5 W! }! U0 V" E) p& |
Vincent J. J. Martin and William W. Mohn ...................................... 315
8 P0 Q c" }5 |; l& T: W, v34 Rapid Amplification of Genomic DNA Sequences Tagged
. A! `+ j0 z4 N# eby Insertional Mutagenesis
$ v4 {4 L# C' y: q6 jMartina Celerin and Kristin T. Chun ................................................ 325) s' o: O( _ p9 I
35 Isolation of Large Terminal Sequences of BAC Inserts Based
: Z& Z b8 s* F1 A+ d* Ion Double-Restriction-Enzyme Digestion Followed4 v: K' t+ t6 _" y1 h
by Anchored PCR
- A3 A* L0 y: iZhong-Nan Yang and T. Erik Mirkov ............................................... 337. k1 H, U/ n0 E5 y" W# D: ~; H/ A
2 N7 p$ [3 \* t; G8 |36 A “Step Down” PCR-Based Technique for Walking6 C Y5 F& T, T4 Y) i u6 ^
Into and the Subsequent Direct Sequence Analysis
# V1 m9 g" A. \; J3 |8 s, Y9 Aof Flanking Genomic DNA: w( i; e5 \* `4 L6 j7 m
Ziguo Zhang and Sarah Jane Gurr .................................................. 343
0 t2 @0 L+ S$ P. H+ q Y* W3 ?7 ePART V. LIBRARY CONSTRUCTION AND SCREENING" i: @" ]; I w2 M5 D) P
37 Use of PCR in Library Screening: An Overview
6 T+ O# ?' g+ W2 o3 Z4 A/ a; o4 ?Jinbao Zhu .......................................................................................... 353
' @; W! |5 R6 o" B9 S/ q38 Cloning of Homologous Genes by Gene-Capture PCR
/ J* C8 }& E0 R5 u8 W3 wRenato Mastrangeli and Silvia Donini ............................................. 359
4 m) I/ Y9 }% g( F39 Rapid and Nonradioactive Screening of Recombinant Libraries by PCR. R$ I/ n6 g5 A+ ^
Michael W. King ................................................................................. 377& T/ O, ^9 G' e' K
40 Rapid cDNA Cloning by PCR Screening (RC-PCR)
7 W+ `5 T) e2 D3 V% t; IToru Takumi ....................................................................................... 385; O. R1 C; u' q" h7 P5 b
41 Generation and PCR Screening of Bacteriophage λ Sublibraries& B3 Q2 H- W& d: G7 R
Enriched for Rare Clones (the “Sublibrary Method”)
# n- Z4 y8 o$ I$ i$ n+ \7 P/ T/ yMichael Lardelli .................................................................................. 391
+ ^# N0 o, i0 |% a8 a7 I42 PCR-Based Screening for Bacterial Artificial Chromosome Libraries
5 Q& Q2 N9 P. n% T: v4 OYuji Yasukochi ................................................................................... 401! z/ _& Y) m5 _6 ~
43 A 384-Well Microtiter-Plate-Based Template Preparation
5 \4 W( I. i" W1 o5 Kand Sequencing Method
0 o" x. U2 t/ g' B0 fLei He and Kai Wang ......................................................................... 411
8 F( j/ `) P0 N: k0 Z44 A Microtiter-Plate-Based High Throughput PCR Product* E& E i5 h2 U& V( E
Purification Method& A1 u) o2 `9 s3 S) r
Ryan Smith and Kai Wang ................................................................ 417
V* W3 N7 v$ p# R! n( ? T/ E$ [2 W3 `2 L5 i' Q- z8 R* G7 {# Z6 t% ]
[hide][/hide]' ]# E; {7 [( i* n
|
附件: 你需要登录才可以下载或查看附件。没有帐号？注册
|
发表于 2011-6-11 11:43
发表于 2011-6-11 14:46
