either way, it depends on your experince. If your feeder cells is established cell line, such as STO, frozen mitomycin C inactivated cells is more convinient. and assure the repeatbility of your result. % i0 Y2 I+ x! iBut it always better to do a couple of times trials, and work out your working protocols.
发表于 2011-12-19 12:53
