ECACC 的细胞培养计数指南--Fundamental Techniques in Cell Culture

dahui

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Fundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition- m  c1 W  {- H7 h2 m/ `  ~

: g. y2 Z/ Y" v7 p3 G8 zIn response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.1 _( L4 n+ r( o0 O( _1 q; Y
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Chapters         Title8 |; ]0 t% I$ o, r2 Y, r
1         Introduction1 b/ G+ u/ j3 ^) P" L7 M4 @
2         Design and Equipment for the Cell Culture Laboratory/ G7 r( W  w8 ]8 Q0 B+ i$ R, ~& u
3         Safety Aspects of Cell Culture
  N2 A/ K' T: Q4 n4         Sourcing of Cell Lines
  \: L6 m6 t7 P9 M5         Cell Types & Culture Characteristics
7 S2 ?  h8 M  A5 \6         The Cell Environment (including types of culture medium)
' \, m, M8 ?, c' s) {* p+ S, e& B% H3 k7         Cryopreservation and Storage of Cells; l1 l' v4 W" H0 }
8         Good Cell Banking Practices
& G8 `0 S2 ~' e" S) C9         Quality Control Considerations in Cell Culture
: N) Q3 }- p, b0 v10         Authentication of Cell Lines
% s2 [& t6 a& D  g( c- R' U3 X11         Alternative Culture Systems
' v! d8 Z8 p4 ~' i12         Basic Techniques - The “Do’s and Don’ts” of Cell Culture! ~* p% z% D+ E) o
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Protocols         Title; G* m% v& I* K2 K2 m. y7 m- }
1         Aseptic Technique and Good Cell Culture Practice, N. Q+ Z8 ^! U7 D, M8 z
2         Resuscitation of Frozen Cell Lines; ], ~# ^1 K1 N% {- J, l, T
3         Subculture of Adherent Cell Lines- W6 T- S# i# r8 q6 V+ C% X
4         Subculture of Semi-Adherent Cell Lines# t4 o( g5 W% _' S
5         Subculture of Suspension Cell Lines3 S6 ?3 W$ X" k* ]" r! h$ J
6         Cell Quantification
/ g& B3 K  ~- W8 A2 D7         Cryopreservation of Cell Lines6 Q  {' H( a' ~1 M5 t7 p
8         Testing for Bacteria and Fungi in Cell Cultures
% u% f" k- u" j" Q9         Testing for Mycoplasma by Culture Isolation" j2 F0 B7 h& m) t
10         Testing for Mycoplasma by Indirect DNA Stain
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% k* u& _' K1 q! GGlossary of Terms
" U: S3 P1 F$ E3 H[hide][/hide]

tjbone

学习谢谢了！！！！！

langziforever

非常感谢！

hormone008

good
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thanks
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cc1986

学习学习

henryjia

GOOD

hany2007

谢谢分享

aminhair

看看

aminhair

晕，sigma的

bllx58

太好了，感谢楼主