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本帖最后由 细胞海洋 于 2011-9-1 22:04 编辑 - B2 {, a$ v; k
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( I$ U7 X$ F# Q( V7 g9 h! TFundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition7 B/ z* H9 i; e5 |% q& E
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In response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.
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7 X; | p: w0 J6 qChapters Title! J1 q) T# |4 G( m3 w" Y! T7 q
1 Introduction3 z. a% I1 y% Y: Y9 s! B
2 Design and Equipment for the Cell Culture Laboratory6 @2 }4 J. r; v# V* {1 n
3 Safety Aspects of Cell Culture
6 Y% d' s4 }& {! {4 Sourcing of Cell Lines R0 F- P# E2 `* k5 R; D: O
5 Cell Types & Culture Characteristics
( w2 }3 L: q X/ U% w6 The Cell Environment (including types of culture medium)' o4 h; r0 K1 I! Y
7 Cryopreservation and Storage of Cells5 `* {0 k& X) p9 X8 o2 F
8 Good Cell Banking Practices
5 p2 F/ Q0 X/ c7 ^" N9 Quality Control Considerations in Cell Culture1 J5 t, {2 T/ ?1 s
10 Authentication of Cell Lines
1 c* M/ b) R3 Q' d; {11 Alternative Culture Systems1 d3 D, K$ b% b, s9 ?3 F6 m
12 Basic Techniques - The “Do’s and Don’ts” of Cell Culture" w9 i! w9 g. U8 y4 q
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Protocols Title- p% u7 _' Q) ?/ M
1 Aseptic Technique and Good Cell Culture Practice f9 h7 W; E1 Y% l6 ?# B
2 Resuscitation of Frozen Cell Lines Z+ R* |% v! A$ j! @% R. H
3 Subculture of Adherent Cell Lines, l: w' c( ?% g2 F) a% C
4 Subculture of Semi-Adherent Cell Lines& D3 f9 B0 z$ {, m6 K
5 Subculture of Suspension Cell Lines
' F5 e1 Y0 A' C5 f0 J7 X: r6 @: \( V, }- `6 Cell Quantification
7 C1 i( N4 j" I7 Cryopreservation of Cell Lines
5 u( T- f+ I' D: P& v8 Testing for Bacteria and Fungi in Cell Cultures
$ a4 `/ \7 r! `9 p8 V8 F5 H5 ^% S9 Testing for Mycoplasma by Culture Isolation
- e: A' {- P: T! |10 Testing for Mycoplasma by Indirect DNA Stain
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* ]! {* D/ I! j+ i. Y! fGlossary of Terms; P2 d8 F F) \; C# ]% y( W
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发表于 2011-9-1 15:17
