ECACC 的细胞培养计数指南--Fundamental Techniques in Cell Culture

dahui

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' Z  p/ r3 k/ v. R7 a" L, @Fundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition; i, B; n8 g$ ^
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In response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.
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! q) E7 p) h( X% U4 fChapters         Title1 G6 v/ @; ~: m- v7 V' G3 k: s" I
1         Introduction
& ~7 J) L* I8 \. z" [' F3 u: d2         Design and Equipment for the Cell Culture Laboratory+ |4 N% i5 F$ ~: R' X3 d
3         Safety Aspects of Cell Culture
9 Z, m- l/ H# T6 V3 j# h4         Sourcing of Cell Lines
+ j: }. \6 a7 @; S7 e+ W5         Cell Types & Culture Characteristics
# A* [; _$ k5 L& X  Q. t6         The Cell Environment (including types of culture medium)6 A/ r6 Q0 ^* o* V2 Z; d) W1 _
7         Cryopreservation and Storage of Cells$ {, `# G) F6 a+ V0 c% l
8         Good Cell Banking Practices
! t( ~8 n1 j5 f1 H1 |9         Quality Control Considerations in Cell Culture
) q4 V5 e6 Q1 ?  M  c  A, W* n10         Authentication of Cell Lines* W' y3 V' M, G
11         Alternative Culture Systems* D! z5 J/ J# u- ~
12         Basic Techniques - The “Do’s and Don’ts” of Cell Culture
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Protocols         Title
0 E( U* x; e' o5 A) g- a1         Aseptic Technique and Good Cell Culture Practice$ o% V% @% r& V+ H
2         Resuscitation of Frozen Cell Lines
) s$ u' o/ m& [: \/ k3         Subculture of Adherent Cell Lines
& p" `! ]6 C/ R4         Subculture of Semi-Adherent Cell Lines
" f- ]. t3 y# `5         Subculture of Suspension Cell Lines+ |7 n: f8 J8 |- l& @, q
6         Cell Quantification
6 z  \4 N' H* ~; b* {: X7         Cryopreservation of Cell Lines
) D4 s4 [7 H$ `( r+ G  o8         Testing for Bacteria and Fungi in Cell Cultures
% Z+ x+ \0 B1 h* S- I9         Testing for Mycoplasma by Culture Isolation
9 t" P6 j6 V6 i: ?10         Testing for Mycoplasma by Indirect DNA Stain
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/ H$ ~/ t& _. W8 GGlossary of Terms1 c1 B6 b# T. ?8 K0 e) B( s% Y
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tjbone

学习谢谢了！！！！！

langziforever

非常感谢！

hormone008

good
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cc1986

学习学习

henryjia

GOOD

hany2007

谢谢分享

aminhair

看看

aminhair

晕，sigma的

bllx58

太好了，感谢楼主