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本帖最后由 细胞海洋 于 2011-9-1 22:04 编辑
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Fundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition
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$ P8 e# m0 U! A( ?! ?$ zIn response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.
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Chapters Title$ I9 X! x* P# m
1 Introduction
- d5 t5 e% U1 U" U8 }! ~3 w2 Design and Equipment for the Cell Culture Laboratory5 B8 O" b& N K* I
3 Safety Aspects of Cell Culture# d$ v/ _& }7 a+ Z7 S; v6 R' @
4 Sourcing of Cell Lines
/ w; e( F2 {; R& q7 B8 M9 E* G5 Cell Types & Culture Characteristics7 D" y4 L- {9 ?8 E. q8 D5 ?
6 The Cell Environment (including types of culture medium)
7 n9 C+ {( ^, \3 j! B% C- P7 Cryopreservation and Storage of Cells
# w7 @; `: C$ z2 J( M8 Good Cell Banking Practices
1 S. E* [: G6 f$ p& `9 Quality Control Considerations in Cell Culture& L5 S* B$ N3 I( O; E9 l' ^8 Y# k
10 Authentication of Cell Lines5 R/ K3 h. v% n1 ?$ `; ?. H9 U; B
11 Alternative Culture Systems
7 @/ D+ i, o; K; {* ]/ Y' Q12 Basic Techniques - The “Do’s and Don’ts” of Cell Culture2 I4 i1 C& V2 Q* W Q5 m- f, \5 u
5 T. |5 k# y4 A k: ? `8 H S
Protocols Title$ \4 t% }6 K& n" Q
1 Aseptic Technique and Good Cell Culture Practice
2 E/ ]+ `" F7 `! T2 Resuscitation of Frozen Cell Lines! p- U- j4 V8 B& m
3 Subculture of Adherent Cell Lines |* s( O0 `5 J6 `1 K p: s5 b, F7 M
4 Subculture of Semi-Adherent Cell Lines% U1 j3 f7 J0 z6 m+ p
5 Subculture of Suspension Cell Lines
. V5 F4 ~% p1 t9 n( `: A" o L) b6 Cell Quantification1 Y0 ]: q0 Q B! Y
7 Cryopreservation of Cell Lines
) E V6 F5 i$ W0 r2 s9 Z1 u8 Testing for Bacteria and Fungi in Cell Cultures
' n# d4 ~- X1 d* d9 Testing for Mycoplasma by Culture Isolation
' W, Y" J2 F \0 m0 X" q. Y3 }10 Testing for Mycoplasma by Indirect DNA Stain
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Glossary of Terms. D/ ^! k7 g7 p+ H
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发表于 2011-9-1 15:17
