|
- 积分
- 1343
- 威望
- 1343
- 包包
- 387
|
本帖最后由 细胞海洋 于 2011-9-1 22:04 编辑 $ Z, O9 W6 a3 f7 @" V
% U/ K7 g9 o; F1 s* {7 x8 [. e) G
- \* C4 K, e) ^3 NFundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition W7 p. x6 y% ?1 w- r+ A4 ?
5 j# q2 A. e- B2 w% D. K( }. QIn response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.
. E% ^( ]; S$ h* S# Q& v! P( a. m2 E$ k
Chapters Title
/ E8 }0 Z% M, }1 b3 S, v) p1 Introduction+ T$ O8 G* _, A) i, H. @
2 Design and Equipment for the Cell Culture Laboratory
" m3 Q- z* N* Z( y! L# Z3 Safety Aspects of Cell Culture; T. W- g& Y! S8 n3 s% T
4 Sourcing of Cell Lines# R: G% l+ f, N3 _" z
5 Cell Types & Culture Characteristics8 X2 D5 f, O7 Y2 e; E+ h9 s P% z
6 The Cell Environment (including types of culture medium)
5 V# I. \% `% V( r7 O" O/ V. _/ I( j7 Cryopreservation and Storage of Cells) c+ w: A/ E/ O/ q7 k9 O
8 Good Cell Banking Practices
- v% l( z2 J, H& d8 E# j1 s+ l4 F9 Quality Control Considerations in Cell Culture
% G- w1 r) ~ Q10 Authentication of Cell Lines/ I' @, O( F2 U" r. y6 L$ `$ S
11 Alternative Culture Systems
e% D3 |6 z0 Q$ B12 Basic Techniques - The “Do’s and Don’ts” of Cell Culture
2 e) N1 }1 F* a: R. m" q) w; C; O
5 c% Q0 S6 q! x1 RProtocols Title J6 i; H; W% _; N$ ?
1 Aseptic Technique and Good Cell Culture Practice
* G4 | y0 Y ^* M: `# K2 Resuscitation of Frozen Cell Lines, Q' q; m8 P, a- G+ B% d
3 Subculture of Adherent Cell Lines
7 M; N9 S2 p7 y( L4 Subculture of Semi-Adherent Cell Lines9 V) w$ n6 y# U8 a
5 Subculture of Suspension Cell Lines
0 x" c' I/ y" U6 t: a0 ]. @6 Cell Quantification
! M2 t, M+ v# }9 u% o M7 I7 Cryopreservation of Cell Lines
( q' n: Z% q* R- k8 Testing for Bacteria and Fungi in Cell Cultures
, ^" J0 r8 O, N% a9 x' q. M' F5 \# h9 Testing for Mycoplasma by Culture Isolation) v5 |8 R- }4 ~& q$ g {$ ]& X
10 Testing for Mycoplasma by Indirect DNA Stain
, B6 b4 c3 ]8 c2 y" Q3 h- n$ p& A# J0 _6 U! Z l; |% E/ _
Glossary of Terms
/ V \( Z6 R9 f! T8 g[hide][/hide] |
附件: 你需要登录才可以下载或查看附件。没有帐号？注册
-
总评分: 威望 + 5
包包 + 10
查看全部评分
|
发表于 2011-9-1 15:17
