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- 积分
- 164
- 威望
- 164
- 包包
- 1030
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Our electron microscopy showed that although the mouse oocyte
\0 z& U* z- g% x$ G) g+ Yhad a wide perivitelline space and few short cell junctions on the
# A/ {" }- z* @' uegg surface, the rabbit oocyte showed no perivitelline space but numerous
6 `! [0 G8 f5 x8 o n! Zlong gap junctions that projected deeply into the egg cortex.6 {. f2 j9 k* x2 M1 }
As a matter of our experience, it was much more difficult and took
, ]6 J5 o5 q: d( Emuch longer time pipetting to remove cumulus cells from rabbit than
* @. }( Z1 y" ~! e0 Nfrom mouse oocytes. Shear stress generated with use of a rotating
$ b- o# p+ X. L! p& W4 O1 g; Cwall vessel or by pipetting induced stress-activated protein kinase/) M- E. S* g' L u
jun kinase phosphorylation that caused apoptosis in mouse embryos: C) a7 X1 O' a( |
(41, 42). It is therefore deduced that because the deep-set junctional
1 w- f$ E& X6 C. w7 J/ \$ D: gcomplexes firmly anchored the oocyte and corona cells, although the
5 M9 @6 i* e. v0 @( s5 @' Lremoval of corona cells damaged plasma membrane of rabbit oocytes,; I# u X! M( Z9 r& G0 Z* V0 j
leading to their degeneration or lysis, the long-time intensive
( f* q$ Z2 V/ A5 b1 B1 R$ zpipetting during cumulus denudation induced oocyte apoptosis and! O. C+ j2 l% s6 ?9 M
inability to mature in vitro.
7 Q# ~ `4 o$ | F3 u请问黄色字幕这两句怎么翻译呢？谢谢! g8 v0 R* k- f& {9 }$ |9 k9 ?. K
* @, H+ S6 Z, X! o) I
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发表于 2011-9-22 20:02
