请求 精子如何计数

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4 x5 {: J0 h0 H* Z: ]Cauda epididymal sperm counts For sperm count analyses, each cauda epididymis was placed in a clean 1.5 mL microcentrifuge tube containing 500 lL distilled water and minced using a sterile Squisher_ (Zymo Research Corp., CA, USA). The tube was vortexed briefly and a 100 lL aliquot of the resulting suspension was placed in an IDENT-stain tube (Hamilton-Thorne Biosciences) with 100 lL distilled water. The tube was again vortexed and incubated for 3 min at room temperature. A 5 lL aliquot was placed on a 20 lm chamber slide (X-CEL; Hamilton-Thorne Biosciences), and spermatoazoa were counted using the IDENT option of the Oval Head Toxicology programme (Hamilton-Thorne Biosciences). A minimum of 40 scans per individual were recorded.