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本帖最后由 细胞海洋 于 2011-10-8 20:37 编辑
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2 k; \+ W6 ~% I: ?# cA guide to commonly used techniques for protein interaction analysis. ' \4 I1 N6 y+ i1 n7 D# | u8 K* m# ]
Designed to help you determinine which technique or combination of techniques is best suited for your needs.1 G. L' @5 I1 ]. \# ]6 c, w
- z2 D/ Y; Y0 a7 @2 [: A kGuide Contents2 C, V- i# q) K/ q3 Y
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Yeast Two-hybrid System
i* C5 E4 z: Q* nMammalian Two-hybrid System
. w: e; ]7 q: @3 o% SPull-down Assays
# G" W6 T9 u9 _Co-immunoprecipitation
( A$ i9 f0 V8 \: lCo-localization/FRET/BRET
' P% o5 n% u9 q% Y6 R1 `Chromatin Immunoprecipitation
/ t; P! S- Z" u& R: dElectrophoretic Mobility Shift Assay3 v" }' K; _$ P# {
Appendix4 y% L8 ] H* V! ?: |
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