PDF电子书：Human Cell Culture Protocols 3e （2012） Humana press

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4 B& T8 u9 |8 y( O! I0 ]PDF 电子书： Human Cell Culture Protocols 3e 2012 Humana Press
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9 I0 [  a7 ]  ^- i1 n! [- X5 h' E9 K主要内容如下：; R9 Q6 x; \: T1 O2 k& E+ D3 I
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Contents2 O: v0 d- a6 i
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  vii
  z5 g' s, D) T9 w. {& E( aContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  xi
0 O7 a( g( f8 S; R  s" \1 b  1  Introduction to Cell Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1+ n2 j& Z7 X, [3 |7 W
Christina Philippeos, Robin D. Hughes, Anil Dhawan, / d0 g9 Y- O/ m8 ^- ^5 H' q
and Ragai R. Mitry
0 l7 D! x% n- K+ P9 ~5 A* U  2  Isolation and Cultivation of Dermal Stem Cells that Differentiate
* e/ A; T8 E: P" y  ~into Functional Epidermal Melanocytes  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  15
. ?( H; r+ d+ d# j- B8 M1 r. R& YLing Li, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn' Z$ y0 R2 B! V6 z0 `( n3 J$ O& U0 g8 F
  3  Isolation, Cultivation, and Application of Human Alveolar
% y( J+ ]% y9 Y, K9 I* nEpithelial Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  31
0 C, r& l, Q' X- S! `Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, # t9 X" P- I2 E# t. X
Hanno Huwer, and Claus-Michael Lehr
/ z, V* u* Z/ B) `1 a+ W3 E$ j: C  4  Culture of Parathyroid Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  432 y; y, c, B7 k4 P. ^
Peyman Björklund and Per Hellman
7 f6 `0 K" M0 _  5  Functional Analysis of Human Islets of Langerhans Maintained * V- L* g# b( b# ^/ U
in Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  55
; U/ y0 [  y# }+ ~6 @4 s; MShanta J. Persaud, Bo Liu, and Peter M. Jones
8 J5 n& z+ d* s+ _0 Z  6  Conversion of Non-endocrine Human Pancreatic Cells ) I+ Y, K$ x6 i+ l) p/ B
to Insulin-Producing Cells for Treatment of Diabetes . . . . . . . . . . . . . . . . . . . . . . .  73
, R; Y/ f% R- z- P. f+ TMin Zhao and Guo Cai Huang
! f: ?; i2 E# y2 ?) ~3 y  7  Evaluation of Cytochrome P450 Activities in Human ; ^- L0 Q, O) a2 j1 F9 z8 e+ Q
Hepatocytes In Vitro  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  87
# `, `) e) f/ }5 t1 e0 f. j* N  wMaría José Gómez-Lechón, Agustín Lahoz, José V. Castell,
& ~2 X/ B6 ^+ `) band María Teresa Donato
2 p( Z( R9 _" g+ r; T: x& v  8  Human Hepatocytes: Isolation, Culture, and Quality Procedures  . . . . . . . . . . . . . .  998 Q# }# e$ l! [6 g6 V% G7 S" U
Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka, ' F9 t) _( [) i  h6 {4 V( p
Peter Büchler, Michael Schoenberg, Jörg Kleeff,   C! d9 @& p% S. G# b4 H* k( q4 G
Wolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy, " w( p5 |5 u2 o3 g; \
Jan Hengstler, and Andreas K. Nussler % A  U! P& c& b/ w3 @: _
  9  Preclinical Testing of Virotherapeutics for Primary and Secondary + ?2 z, w2 J4 e% [. G" C8 b* W
Tumors of the Liver  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  121
2 z# {2 _/ c8 N& b3 d& V0 n; PMartina Zimmermann, Timo Weiland, Michael Bitzer,
( S% Q+ h+ j: R  G9 I, x" H  @; Fand Ulrich M. Lauer) \2 u' _! r/ d, l! l
10  Isolation and Functional Studies of Human Fetal Gastric
- B0 h5 q0 `* M4 @/ _% x& m, {Epithelium in Primary Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137, j. I# L, f3 ]) H( c. |1 u
Pierre Chailler, Jean-François Beaulieu, and Daniel Ménard8 j- Z' Q3 K: a7 A
11  Isolation, Characterization, and Culture of Normal Human   J" i( s* p/ N/ b
Intestinal Crypt and Villus Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  157
/ c. E0 S2 I( ]% |# U( @% ~Jean-François Beaulieu and Daniel Ménard2 _6 |  y( q; Y9 P6 D9 L5 U
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12  Primary Culture of Human Renal Proximal Tubule Epithelial Cells . q5 `/ v( r" h7 S6 u
and Interstitial Fibroblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  175& ]2 p) h5 p( V0 X# R
Claire C. Sharpe and Mark E.C. Dockrell5 ~* F* z6 F* c9 @
13  Glomerular Epithelial and Mesangial Cell Culture $ _, w) U0 }: q7 z: b
and Characterization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  187
  y! _0 J" u2 Y1 t# a" p9 [& jHeather M. Wilson and Keith N. Stewart6 Y8 f+ k7 w" [8 [
14  Culture of Isolated Human Adipocytes and Isolated Adipose Tissue  . . . . . . . . . . . .  2038 d/ G5 d$ A3 z9 Q+ B1 _
Kirstin A. Carswell, Mi-Jeong Lee, and Susan K. Fried) b& N  c4 g% Y* e3 q& R
15  Primary Culture of Human Adipocyte Precursor Cells: " {- ^, R' ^0 y2 {( ~, {) K
Expansion and Differentiation  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  215  N6 u1 o& i  m6 X" A& D
Thomas Skurk and Hans Hauner
$ _& W" u' K1 H; E16  Primary Culture of Ovarian Cells for Research on Cell Interactions
2 m( D. s8 J1 k7 Uin the Hormonal Control of Steroidogenesis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  227
, c6 X0 q1 y" I0 Q* U! e, F& I  K/ j# P. GJerzy F. Galas, N) f+ k* w! ~
17  Human Vascular Smooth Muscle Cell Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2514 R! n' a: R5 ^3 H
Diane Proudfoot and Catherine Shanahan
% @3 _- _# l' C18  Culture of Human Endothelial Cells from Umbilical Veins  . . . . . . . . . . . . . . . . . . .  265# A/ O+ o/ C  n& l
Richard C.M. Siow8 O& I% ]; m) e! I. d3 o
19  Human Peripheral Blood Mononuclear Cell Culture for Flow
% _3 z: l6 C' m: [, JCytometric Analysis of Phosphorylated Mitogen-Activated ) o: a( e9 ~8 @, O7 t' b
Protein Kinases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  275
$ p8 Y: D' l0 I4 R. k8 GAthanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou,
* D8 U# e1 K' v/ P3 `. Uand Dimitrios P. Bogdanos  \6 B2 S2 F! w/ [3 n8 d8 u
20 Human CD4+CD25highCD127low/neg Regulatory T Cells . . . . . . . . . . . . . . . . . . . . .  287
9 N  C! @/ p$ \' D, t6 ^* r6 wHaibin Su, Maria Serena Longhi, Pengyun Wang,   ?1 ]: [) w8 x" r
Diego Vergani, and Yun Ma4 h( Q) B) s5 h) t
21  Human Chondrocyte Cultures as Models of Cartilage-Specific
( D# v6 S! ]& u1 A' tGene Regulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  301
% {2 V2 l6 V, h; WMiguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem,
6 r) j  q- x/ B1 o+ V: Q' H; |Ko Hashimoto, Darren A. Plumb, and Mary B. Goldring
! r4 A7 N9 J7 u( f22  Isolation and Culture of Human Osteoblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  337; C0 M7 a1 U+ _  G
Alison Gartland, Robin M.H. Rumney, Jane P. Dillon,
3 a5 H. K+ l9 r% r0 }  h4 J6 E3 k) O/ |and James A. Gallagher& [$ h* S) D. S# {! f7 j
23  Human Osteoclast Culture and Phenotypic Characterization  . . . . . . . . . . . . . . . . .  357
( j+ r- e/ C8 N: F6 }% X0 BAnkita Agrawal, James A. Gallagher, and Alison Gartland
. ~$ ?. |% Q, W1 h& D6 \24  Effects of Temperature Generated from the Holmium: YAG Laser , m* I% v/ b# b/ d: f3 h
on Human Osteoblasts in Monolayer Tissue Culture. . . . . . . . . . . . . . . . . . . . . . . .  377. p1 b, R7 O8 ^. G
Moustafa I. Hafez, Anne Sandison, Richard R.H. Coombs, , d- Z7 {  H% v1 \; B/ a
Ian D. McCarthy, and Al-Shymaa M. Hafez
9 S" e" \, K0 i& Q4 t5 N1 m25  Laser Microdissection Microscopy: Application to Cell Culture . . . . . . . . . . . . . . . .  385
6 C* d3 k) \2 LAhlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia( c$ y' S& f- u& J+ H
26  Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)  . . . . . . . . . .  393
4 }, R2 o6 G1 c# S& F  iRobert Thomas and Elizabeth Ratcliffe$ m# F5 J3 l0 z5 c3 t* U" U
27  Culturing and Differentiating Human Mesenchymal Stem Cells
: C$ k* {# M% n- H9 gfor Biocompatible Scaffolds in Regenerative Medicine. . . . . . . . . . . . . . . . . . . . . . .  4077 z1 {7 x( n% _7 I$ L' ?" W0 r
William R. Otto and Catherine E. Sarraf 5 r/ ?4 W+ B! Q0 K) s
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  427
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MIYAGI

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ines

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tuanzi

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再来一天

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tian2006

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