PDF电子书：Human Cell Culture Protocols 3e （2012） Humana press

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8 l9 v; ?! l. R% S: r6 ?" ^PDF 电子书： Human Cell Culture Protocols 3e 2012 Humana Press, S3 r7 @9 N- a' I

: S6 T' q8 |9 S2 w: @" J! @主要内容如下：' m! a  E  o8 H' b- M

" [. `3 J; e( D, z Contents' L$ Z8 g. ~& l7 w' S3 k
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  vii
3 o# D$ s5 U0 }( }- \  {Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  xi3 y$ q; S1 n+ l5 [) U* h/ b
  1  Introduction to Cell Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1: H3 n# v+ B! G2 o1 `7 w
Christina Philippeos, Robin D. Hughes, Anil Dhawan,
7 g7 u- w$ T, ~and Ragai R. Mitry
! J/ [/ h; P2 U: M9 W* ^3 B. @) H  2  Isolation and Cultivation of Dermal Stem Cells that Differentiate ' K+ n  v1 r, y9 a; v2 g
into Functional Epidermal Melanocytes  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  15/ D# y: ^' s/ l* Z8 e1 A
Ling Li, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn
) O0 \' C; R, o& f7 z( V  3  Isolation, Cultivation, and Application of Human Alveolar , n- T2 _' _, w$ g, o& i7 p! ^2 w
Epithelial Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  319 ]1 u) Y7 i( _4 }, c
Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, $ I! D5 _: m! f7 m
Hanno Huwer, and Claus-Michael Lehr8 ~$ k( \! m4 }7 e+ m) z
  4  Culture of Parathyroid Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  43
& |! \3 J9 w' U# J( z- w0 qPeyman Björklund and Per Hellman
; w) V) a) [) \' `$ z  5  Functional Analysis of Human Islets of Langerhans Maintained
, g5 w0 L, A7 Z8 O' S* v, ]in Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  558 y' I6 ^  X3 B. G
Shanta J. Persaud, Bo Liu, and Peter M. Jones 8 J5 E: b/ l# B$ e; o
  6  Conversion of Non-endocrine Human Pancreatic Cells ( h$ }/ N  C. e7 g2 m0 d* x
to Insulin-Producing Cells for Treatment of Diabetes . . . . . . . . . . . . . . . . . . . . . . .  73
! J5 T, Y- R# r* }1 g( FMin Zhao and Guo Cai Huang
' r8 x1 Z$ \1 I. k  7  Evaluation of Cytochrome P450 Activities in Human
7 V' W! }' V4 _Hepatocytes In Vitro  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  877 {5 f1 Y. C# s" h
María José Gómez-Lechón, Agustín Lahoz, José V. Castell,
& V2 g. z# g9 e& @& G( O- ]and María Teresa Donato; y1 Y  l: t, e, _
  8  Human Hepatocytes: Isolation, Culture, and Quality Procedures  . . . . . . . . . . . . . .  99. m8 Q0 j( t# v3 f9 y5 C/ F) a
Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka, $ A5 e0 X) D9 T* C/ Q1 [
Peter Büchler, Michael Schoenberg, Jörg Kleeff, 1 b+ x- X8 q8 t' ^2 D% m% x
Wolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy,
1 e+ U* o: A5 EJan Hengstler, and Andreas K. Nussler
) ^0 N2 ~, l! a- r- U7 E  9  Preclinical Testing of Virotherapeutics for Primary and Secondary - [  Z9 g$ P+ ]2 S( c/ |
Tumors of the Liver  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1216 U$ _' m% Y* P/ y
Martina Zimmermann, Timo Weiland, Michael Bitzer, . p3 Z- v4 K  b- N' U+ i
and Ulrich M. Lauer
* b/ S* W9 M* c' C  }/ ?10  Isolation and Functional Studies of Human Fetal Gastric 4 z, b* j6 o3 u: @( Y, b4 n
Epithelium in Primary Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137
; a1 T0 C& T& v4 xPierre Chailler, Jean-François Beaulieu, and Daniel Ménard
1 I* O, S, f5 v" j11  Isolation, Characterization, and Culture of Normal Human ; }# a$ d9 w7 l* R" i8 i3 q
Intestinal Crypt and Villus Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  157# _: z- j" P% }1 A' ^) f+ h3 ^
Jean-François Beaulieu and Daniel Ménard$ T. x# a: ?: s& k$ P
x Contents
4 L4 `! v" V, b9 @12  Primary Culture of Human Renal Proximal Tubule Epithelial Cells
+ |% W* a9 V0 _, C, J: f* Z7 band Interstitial Fibroblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  175
. h" ~+ ^1 r+ A% k) d2 R% h$ l- zClaire C. Sharpe and Mark E.C. Dockrell; F& ^4 h  B3 F* C+ h7 C
13  Glomerular Epithelial and Mesangial Cell Culture
9 a" Y5 b9 s8 q/ p6 w7 D& t& q7 oand Characterization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  187
+ R& j9 s4 ~5 K- R" h7 WHeather M. Wilson and Keith N. Stewart
9 s: t+ z2 |! k# N  y8 S2 d" M  v14  Culture of Isolated Human Adipocytes and Isolated Adipose Tissue  . . . . . . . . . . . .  203
2 [' N7 m8 ^5 P, H0 B. jKirstin A. Carswell, Mi-Jeong Lee, and Susan K. Fried
4 s& s! b1 D+ B. w" ^15  Primary Culture of Human Adipocyte Precursor Cells:
$ ~% h% v/ s, \! b5 SExpansion and Differentiation  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  215
: i% L" Q2 a/ Y( E+ s, V: \- oThomas Skurk and Hans Hauner  [( g9 U9 x# Y) `
16  Primary Culture of Ovarian Cells for Research on Cell Interactions 3 ]% Y% k( H. C
in the Hormonal Control of Steroidogenesis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  227
; Q8 a! I$ d1 Z+ Y8 ~* y3 m& {2 W3 E3 ~Jerzy F. Galas; t( c# d7 S. k+ K8 P
17  Human Vascular Smooth Muscle Cell Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . .  251
2 N* j9 t3 A, T& d! lDiane Proudfoot and Catherine Shanahan2 z5 k* o- ^0 v; U
18  Culture of Human Endothelial Cells from Umbilical Veins  . . . . . . . . . . . . . . . . . . .  2654 ?; `5 G3 l9 e6 [5 K) q
Richard C.M. Siow
- C. ~/ n5 v& b8 O" e' l3 h19  Human Peripheral Blood Mononuclear Cell Culture for Flow
3 q( U3 ?% V, t3 v$ rCytometric Analysis of Phosphorylated Mitogen-Activated
2 Y) W2 H; z, ~! l/ _: F; ?: MProtein Kinases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  275
. S" M' {" w3 @Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, , y; c2 V8 B; o, \
and Dimitrios P. Bogdanos5 U5 Q, Z) z+ c; x# e
20 Human CD4+CD25highCD127low/neg Regulatory T Cells . . . . . . . . . . . . . . . . . . . . .  287
6 Y9 y1 x% q: M) N; D9 iHaibin Su, Maria Serena Longhi, Pengyun Wang,
4 k/ E, a4 T/ XDiego Vergani, and Yun Ma
; ~* ^( @0 k# o6 V' {' x% @1 o21  Human Chondrocyte Cultures as Models of Cartilage-Specific
0 G) H' Z5 K, K' }Gene Regulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  301
+ i9 D$ Q$ ^' n7 l( G2 fMiguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, 0 i. T5 B5 B% i# j9 r+ `- i
Ko Hashimoto, Darren A. Plumb, and Mary B. Goldring% F/ i- @7 h6 _% m) D: q% Z* j0 l
22  Isolation and Culture of Human Osteoblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  337
( f9 s3 ]% s* `9 gAlison Gartland, Robin M.H. Rumney, Jane P. Dillon, 1 l9 ]0 [$ G% [/ H8 m( o
and James A. Gallagher1 o( @* O3 `$ M4 x" r) r- L; k
23  Human Osteoclast Culture and Phenotypic Characterization  . . . . . . . . . . . . . . . . .  357
, C  b9 h2 l! F* G+ tAnkita Agrawal, James A. Gallagher, and Alison Gartland
: }/ r1 E2 n2 D24  Effects of Temperature Generated from the Holmium: YAG Laser . u  ?! a/ Z: E  ~" I& a" O, |
on Human Osteoblasts in Monolayer Tissue Culture. . . . . . . . . . . . . . . . . . . . . . . .  377
9 A& D% S) {; w6 |' x4 W' pMoustafa I. Hafez, Anne Sandison, Richard R.H. Coombs,
4 X$ q2 m& ^) k5 aIan D. McCarthy, and Al-Shymaa M. Hafez
+ y0 E9 t- l5 p7 P0 p7 \. C. }6 |! ^25  Laser Microdissection Microscopy: Application to Cell Culture . . . . . . . . . . . . . . . .  385% ?/ o3 A: `3 |  A
Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia# d2 }' |+ H- ~/ r  F
26  Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)  . . . . . . . . . .  393, t$ g, A' Q  }1 @( M' D
Robert Thomas and Elizabeth Ratcliffe1 l% Q) q$ S" B9 g# E7 |( `3 o6 u6 N, {
27  Culturing and Differentiating Human Mesenchymal Stem Cells
- ^6 ^; N0 P, S: c* Z$ |for Biocompatible Scaffolds in Regenerative Medicine. . . . . . . . . . . . . . . . . . . . . . .  407
% {, ?0 W  w( M8 V, R2 Y, i5 @William R. Otto and Catherine E. Sarraf ) j+ c1 I/ W  {6 \  C4 F0 d2 Y
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  4278 P  F3 {, p* d/ Y
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