PDF电子书：Human Cell Culture Protocols 3e （2012） Humana press

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4 a3 g+ y' E" A" L5 oPDF 电子书： Human Cell Culture Protocols 3e 2012 Humana Press8 Y6 ?: W; k' m6 i- }' R

" X# M0 T0 S1 v主要内容如下：
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" ~  X9 m5 X6 C; e9 C, u, p3 L Contents
* n4 h. q% a' U$ qPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  vii
& O& b9 v  J) q+ n- F% V* cContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  xi
2 d* h# R0 m. H, a$ N  1  Introduction to Cell Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1, L; ~" C! {  |$ X9 Z& o
Christina Philippeos, Robin D. Hughes, Anil Dhawan,
" q7 w) o/ M3 K& {& m# F* k" }! band Ragai R. Mitry
% e0 @! v2 u0 H- P4 d, t; t( i  2  Isolation and Cultivation of Dermal Stem Cells that Differentiate + G0 m% J2 Y4 u/ g2 `/ E1 s
into Functional Epidermal Melanocytes  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  15
' E* q: d$ b5 _Ling Li, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn
6 ]9 N. t" d% H- j* J) v  3  Isolation, Cultivation, and Application of Human Alveolar
! B, p: }9 i* Z4 {! \9 F. ~4 o! kEpithelial Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  31
5 s/ u2 I6 o8 ?Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, . L/ R- a0 U8 ~. a6 h6 {0 \! M
Hanno Huwer, and Claus-Michael Lehr
) r. m* b  c, K% |% E/ j  4  Culture of Parathyroid Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  43
/ Z3 j/ C) P0 [5 F7 ZPeyman Björklund and Per Hellman
5 o+ p% U2 m+ c7 N! @  5  Functional Analysis of Human Islets of Langerhans Maintained 2 C2 F$ ~5 Y& b. F
in Culture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  55
4 k" V" i# G" q% L8 T' d2 }+ IShanta J. Persaud, Bo Liu, and Peter M. Jones ( w, j* u! a  u* c
  6  Conversion of Non-endocrine Human Pancreatic Cells ' A7 M9 p, [, k+ ~
to Insulin-Producing Cells for Treatment of Diabetes . . . . . . . . . . . . . . . . . . . . . . .  73, n# _# [& }9 `; ~! s
Min Zhao and Guo Cai Huang& v  l5 C9 _( O; Z0 H4 b
  7  Evaluation of Cytochrome P450 Activities in Human
' g4 }+ Q* @- S; ?3 ~! h% V$ BHepatocytes In Vitro  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  879 v: s# Y) ~" E
María José Gómez-Lechón, Agustín Lahoz, José V. Castell,
3 }3 C* N1 Q8 ~7 {  }" y! `* M; Qand María Teresa Donato7 z5 L) j, Z7 z1 T4 m7 t
  8  Human Hepatocytes: Isolation, Culture, and Quality Procedures  . . . . . . . . . . . . . .  99& ?( ]: h7 a/ }" K
Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka,
0 X( w/ ]! ^0 l1 t) cPeter Büchler, Michael Schoenberg, Jörg Kleeff,
2 W/ P! M1 c9 HWolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy, & a2 E. k; H) l# k6 D. i
Jan Hengstler, and Andreas K. Nussler
! Y2 }7 Y/ Y  K6 D, G' i8 M7 O  9  Preclinical Testing of Virotherapeutics for Primary and Secondary
' f2 ^0 X$ f1 cTumors of the Liver  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  121' V+ H' z' p" c& H
Martina Zimmermann, Timo Weiland, Michael Bitzer,
+ n& B, M) I" F6 C' land Ulrich M. Lauer
& }1 d7 K" c. B$ h10  Isolation and Functional Studies of Human Fetal Gastric / e- q- v& M! E
Epithelium in Primary Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  137
  L9 B, z' B6 T: r2 T5 _Pierre Chailler, Jean-François Beaulieu, and Daniel Ménard
3 n2 x0 b0 G* y  D3 S11  Isolation, Characterization, and Culture of Normal Human + o: D$ S1 {  X3 h
Intestinal Crypt and Villus Cells  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  157
5 O( a: h$ p$ \9 n# u1 s5 NJean-François Beaulieu and Daniel Ménard
- Y1 P9 |% K+ y" N5 qx Contents
- J! a4 t$ w4 c* F, g12  Primary Culture of Human Renal Proximal Tubule Epithelial Cells
( g1 d4 y4 R" S) g# H, |( Band Interstitial Fibroblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  175$ |, G5 [6 ^* f* p
Claire C. Sharpe and Mark E.C. Dockrell+ `0 \+ |, Y, E  f5 [4 Z0 c
13  Glomerular Epithelial and Mesangial Cell Culture
3 D5 J+ _2 `* [2 z/ a7 band Characterization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1878 y. T: |, t% |( ?$ @. |
Heather M. Wilson and Keith N. Stewart
. \$ Y6 }4 u2 V; Z$ C14  Culture of Isolated Human Adipocytes and Isolated Adipose Tissue  . . . . . . . . . . . .  203
- _8 i4 D+ e3 }$ A0 aKirstin A. Carswell, Mi-Jeong Lee, and Susan K. Fried( X; l& ?" G7 ?" t7 {" k. q( D
15  Primary Culture of Human Adipocyte Precursor Cells:
5 m7 D3 f9 }) pExpansion and Differentiation  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2158 g# Y' j" Y" G+ n; L
Thomas Skurk and Hans Hauner: O/ w( `2 B4 `9 v
16  Primary Culture of Ovarian Cells for Research on Cell Interactions ! v( d. t5 q( i3 F# @5 _
in the Hormonal Control of Steroidogenesis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  227* C. I6 n7 u3 Y% ~
Jerzy F. Galas# S# \* r, ?# x+ ]) ?; K! g2 d5 Z, D
17  Human Vascular Smooth Muscle Cell Culture  . . . . . . . . . . . . . . . . . . . . . . . . . . . .  251
/ `1 Q) ?; d% M7 x- }5 j9 E* U! H. EDiane Proudfoot and Catherine Shanahan* J9 j" x3 _5 z9 x* X$ |
18  Culture of Human Endothelial Cells from Umbilical Veins  . . . . . . . . . . . . . . . . . . .  265. Y. A1 H. s# h% S
Richard C.M. Siow
% z# d0 B; O! @$ o0 t" o- n19  Human Peripheral Blood Mononuclear Cell Culture for Flow
. A& w! |* a/ o& Z: WCytometric Analysis of Phosphorylated Mitogen-Activated
. K5 N$ G/ P+ B  ^; e# gProtein Kinases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  2755 o" Q- O& N; c7 v! a
Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, 3 R, I6 i: w0 c5 \
and Dimitrios P. Bogdanos7 r8 w5 Q+ W& w4 x
20 Human CD4+CD25highCD127low/neg Regulatory T Cells . . . . . . . . . . . . . . . . . . . . .  287
' X: l/ n- r7 I- T, _Haibin Su, Maria Serena Longhi, Pengyun Wang, 8 j# e: H* h" d) Z: E  y
Diego Vergani, and Yun Ma
! _8 b' d9 V& F21  Human Chondrocyte Cultures as Models of Cartilage-Specific
  }* R; n" I" l, K3 HGene Regulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  301
5 J; n! i: f' T# y9 lMiguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, 7 X  P- F2 |1 E' {% ]* |8 x) M
Ko Hashimoto, Darren A. Plumb, and Mary B. Goldring5 o. M7 b/ [- {2 ^; a2 R  ^
22  Isolation and Culture of Human Osteoblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  337
& M- \' D$ a  ~9 u5 i; S' K  @7 D( [Alison Gartland, Robin M.H. Rumney, Jane P. Dillon, 3 ~% F& P: t* ]9 g$ l
and James A. Gallagher
/ P8 X0 k4 s: E# S3 n1 {23  Human Osteoclast Culture and Phenotypic Characterization  . . . . . . . . . . . . . . . . .  357" S( T7 i2 }' l# |
Ankita Agrawal, James A. Gallagher, and Alison Gartland
- i2 l, t. d6 p1 s24  Effects of Temperature Generated from the Holmium: YAG Laser
$ e0 s; o5 X) s5 ion Human Osteoblasts in Monolayer Tissue Culture. . . . . . . . . . . . . . . . . . . . . . . .  377
* S* \; h+ m; [Moustafa I. Hafez, Anne Sandison, Richard R.H. Coombs, 4 k$ \0 Q- H/ e1 b/ M. q! B
Ian D. McCarthy, and Al-Shymaa M. Hafez ) K( c) v( v) s* i
25  Laser Microdissection Microscopy: Application to Cell Culture . . . . . . . . . . . . . . . .  3857 z( \' E5 v+ @5 l+ y$ j! q; W
Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia
1 l& A( }3 l6 q7 f& B" ^26  Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)  . . . . . . . . . .  3930 u) t  d; {2 D: g  q- g4 H/ a. x7 a
Robert Thomas and Elizabeth Ratcliffe) F5 @( K' R: A) g% r" S
27  Culturing and Differentiating Human Mesenchymal Stem Cells 8 x, H/ P+ G) s5 F
for Biocompatible Scaffolds in Regenerative Medicine. . . . . . . . . . . . . . . . . . . . . . .  407
0 Z9 k. J' {: q5 uWilliam R. Otto and Catherine E. Sarraf
6 X& P; @) L9 [6 i) ?Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  4279 C, N" O) o' R. E" D! s

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