Shedding light on protein folding Folding of a bacterial protein is di

杨柳

The findings have some immediate applications. Investigators have had difficulty determining whether a particular intermediate for folding is a productive on-pathway intermediate that will result in a proper conformation, or whether something has gone wrong during folding. With PYP, folding by rapid mixing in denaturants and refolding after signaling both pass through the same state, suggesting that this is an on-pathway intermediate.$ Z6 [: q: n: G! ^' D" A! t

# y  v( f7 u2 g4 L/ N# N2 |7 d: ePYP could also improve time resolution for protein-folding studies. Rapid mixing experiments are usually limited to 1 ms resolution, but the light-triggered nature of PYP refolding should cut that down to nanoseconds.
7 w9 V! d4 _! `# A. D  c9 c5 y8 E
6 y( S8 Z4 ^" x# l) s( OReference:
$ {- h; g% y. M) E. @+ R2 N
6 r" `) C  ]8 k2 ]1 N# u+ lLee, B-C., et al 2001. Proc. Natl. Acad. Sci. USA. 98:9062–9067.(Wouter Hoff and his colleagues study pho)