Nat Medicine：生殖研究里程碑成果

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     来自美国麻省总医院（MGH）Vincent生育科学研究中心的研究人员日前从育龄期妇女的卵巢中首次成功分离出可生成卵细胞的干细胞，他们证实这些干细胞能够生成与正常人类卵细胞或卵母细胞相似的细胞。这是自该研究团队于2004年在《自然》（Nature）杂志上发表论文首次证实雌性哺乳动物能够持续生成卵细胞直至成年之后，取得的又一项里程碑式的成果。相关研究论文发表在《自然医学》（Nature Medicine）杂志的三月刊上。(生物通)
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3 Q& |( o7 O; }8 R8 y- ]  {本文的思想来源： 2009年，来自中国上海交通大学的一个研究小组在《Nature Cell Biology》上发表论文称他们不仅成功分离和培养出来自成年小鼠可生成卵母细胞的干细胞（OSCs），并证实将这些OSCs移植到接受卵巢化疗的雌性小鼠体内，可促使小鼠排出成熟卵母细胞，并成功受精，发育形成健康的后代。
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Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women( I: B/ e3 _/ E8 }
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Germline stem cells that produce oocytes in vitro and fertilization-competent eggs in vivo have been identified in and isolated from adult mouse ovaries. Here we describe and validate a fluorescence-activated cell sorting-based protocol that can be used with adult mouse ovaries and human ovarian cortical tissue to purify rare mitotically active cells that have a gene expression profile that is consistent with primitive germ cells. Once established in vitro, these cells can be expanded for months and can spontaneously generate 35- to 50-μm oocytes, as determined by morphology, gene expression and haploid (1n) status. Injection of the human germline cells, engineered to stably express GFP, into human ovarian cortical biopsies leads to formation of follicles containing GFP-positive oocytes 1–2 weeks after xenotransplantation into immunodeficient female mice. Thus, ovaries of reproductive-age women, similar to adult mice, possess rare mitotically active germ cells that can be propagated in vitro as well as generate oocytes in vitro and in vivo.6 {- A' o% E! c% [3 y! o6 j

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hcluo

有啊，你可以好好拜读一下两位大师的文章：: ]4 I$ }: i$ X
1.  Production of offspring from a germline stem cell line derived from neonatal ovaries，Nature Cell Biology 11, 631 - 636 (2009) ) j5 w( K4 S3 K
2. Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women， Nature Medicine (2012) doi:10.1038/nm.26691 K& K6 }4 D% ~* ~- q

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