视频教程-人胶质母细胞瘤神经球培养方法

暮色英雄

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视频教程-人胶质母细胞瘤神经球培养方法
" b3 n$ c  u. G- V3 D* o. eVideo Article
; I* l: M' ^) c2 c* y( s3 m: q* UIsolation and Expansion of Human Glioblastoma Multiforme Tumor Cells
  t& A& g5 t2 S7 lUsing the Neurosphere Assay
: s0 M9 T& a5 {7 a: SHassan Azari1,2, Sebastien Millette1, Saeed Ansari1, Maryam Rahman1, Loic P. Deleyrolle1, Brent A. Reynolds1
% S. P- r$ L3 q% Z) T1 v/ L2 H4 Q1Department of Neurosurgery, University of Florida
% u3 z) [% B: |; n+ r2Department of Anatomical Sciences, Shiraz University of Medical Sciences
0 X1 A0 o6 d/ f; ~% \& ]2 p; k$ jCorrespondence to: Hassan Azari at Hassan.azari@neurosurgery.ufl.edu
! O2 m, w' b: p3 F6 @8 |; x2 q- JURL: http://www.jove.com/video/3633/& o, N8 Q8 w. E) Z
DOI: 10.3791/3633$ z" {* \2 h" a$ r. r* s1 e8 i
Keywords: Neuroscience, Issue 56, Glioblastoma Multiforme, Tumor Cell, Neurosphere Assay, Isolation, Expansion,! J! z$ Y5 h. |# M) l: p
Date Published: 10/30/2011
: S6 C9 Q2 U* v+ kCitation: Azari, H., Millette, S., Ansari, S., Rahman, M., Deleyrolle, L.P., Reynolds, B.A. Isolation and Expansion of Human Glioblastoma Multiforme) ~5 D+ X' e6 l9 ^% l
Tumor Cells Using the Neurosphere Assay. J. Vis. Exp. (56), e3633, DOI : 10.3791/3633 (2011)., g& f& S/ R( C$ p$ d) @
Abstract
( |5 S& C& P* K) i# B8 ]Stem-like cells have been isolated in tumors such as breast, lung, colon, prostate and brain. A critical issue in all these tumors, especially in1 ?. q: l" K( B' s) ~6 I' I
glioblastoma mutliforme (GBM), is to identify and isolate tumor initiating cell population(s) to investigate their role in tumor formation, progression,5 P) z4 I0 z7 d
and recurrence. Understanding tumor initiating cell populations will provide clues to finding effective therapeutic approaches for these tumors. The
) H" D8 `! }* Y# U7 [! `7 E7 }neurosphere assay (NSA) due to its simplicity and reproducibility has been used as the method of choice for isolation and propagation of many of' I2 |6 B8 Q& E! z
this tumor cells. This protocol demonstrates the neurosphere culture method to isolate and expand stem-like cells in surgically resected human$ `( E% b/ m& b9 |
GBM tumor tissue. The procedures include an initial chemical digestion and mechanical dissociation of tumor tissue, and subsequently plating the
  f8 I2 n6 K' w* a' A$ t! t! i: Presulting single cell suspension in NSA culture. After 7-10 days, primary neurospheres of 150-200 μm in diameter can be observed and are ready
" Z; e6 i' `( h! T/ ~for further passaging and expansion.
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下载地址：包含PDF和视频
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liuruii

谢谢楼主，先看看能不能下

leisong12

卡那看那

leisong12

回复 暮色英雄 的帖子
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怎么还要注册网盘，太麻烦了7 o: D' _/ |. J5 w2 ^

wujing

看看咯

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kankan

yuye3000

这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：）

暮色英雄

yuye3000 发表于 2012-3-1 23:01 ! K! h9 Q/ T# M9 @. L$ G6 h
这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：）

6 T8 K7 `( ?0 h) R; |这个肯定是不能说的，我研究了很久才研究出来这个方法，如果告诉你了，那这个网站封锁了这个漏洞，以后就再也搞不到视频了。你可以把视频地址给我，然后我弄到视频后会在这里分享的。

icyfox_fox

谢谢分享

xiaf_0

谢谢分享