视频教程-人胶质母细胞瘤神经球培养方法

暮色英雄

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视频教程-人胶质母细胞瘤神经球培养方法
9 P' i: i5 W2 V/ r, K0 U0 @Video Article
1 p/ Q5 o* a) p' TIsolation and Expansion of Human Glioblastoma Multiforme Tumor Cells1 k* j# A% ~4 O4 A
Using the Neurosphere Assay) u. a) C) X, [
Hassan Azari1,2, Sebastien Millette1, Saeed Ansari1, Maryam Rahman1, Loic P. Deleyrolle1, Brent A. Reynolds1
- G( M, Z8 h- l1Department of Neurosurgery, University of Florida& ?& X- Y: S5 ]& h
2Department of Anatomical Sciences, Shiraz University of Medical Sciences/ ?, }4 A' I3 b
Correspondence to: Hassan Azari at Hassan.azari@neurosurgery.ufl.edu1 e5 u/ I% r6 T1 u8 i) H, L' o: e
URL: http://www.jove.com/video/3633/
5 s* H$ \. i9 M' X; C+ K( KDOI: 10.3791/3633
* W: [4 W% K4 c. u; HKeywords: Neuroscience, Issue 56, Glioblastoma Multiforme, Tumor Cell, Neurosphere Assay, Isolation, Expansion,+ e7 k$ o( e5 e* u, e
Date Published: 10/30/2011
* l7 x6 C8 T. k  e4 G9 TCitation: Azari, H., Millette, S., Ansari, S., Rahman, M., Deleyrolle, L.P., Reynolds, B.A. Isolation and Expansion of Human Glioblastoma Multiforme7 Z1 l0 ~. d' Y% b" C
Tumor Cells Using the Neurosphere Assay. J. Vis. Exp. (56), e3633, DOI : 10.3791/3633 (2011).( q6 [+ j$ B" k& T" o
Abstract
! M8 n3 W3 j2 m- \% G9 T, EStem-like cells have been isolated in tumors such as breast, lung, colon, prostate and brain. A critical issue in all these tumors, especially in
( s! k$ S6 ~$ a0 W, Sglioblastoma mutliforme (GBM), is to identify and isolate tumor initiating cell population(s) to investigate their role in tumor formation, progression,
5 ]; g; e2 H7 u* {4 m- G) ?0 O6 B1 x, yand recurrence. Understanding tumor initiating cell populations will provide clues to finding effective therapeutic approaches for these tumors. The1 S5 a3 V+ |7 g& s# n9 q+ J
neurosphere assay (NSA) due to its simplicity and reproducibility has been used as the method of choice for isolation and propagation of many of
: s$ D. w: |& k% a- E( Zthis tumor cells. This protocol demonstrates the neurosphere culture method to isolate and expand stem-like cells in surgically resected human1 d2 l+ M  f- S/ C( `2 [8 ?0 M
GBM tumor tissue. The procedures include an initial chemical digestion and mechanical dissociation of tumor tissue, and subsequently plating the
: I& N! W: d0 ]: p# `$ {1 s' kresulting single cell suspension in NSA culture. After 7-10 days, primary neurospheres of 150-200 μm in diameter can be observed and are ready$ |5 ?. F) U, O* H& S6 B  V) K
for further passaging and expansion.9 c$ z8 q' R7 F# d$ |
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liuruii

谢谢楼主，先看看能不能下

leisong12

卡那看那

leisong12

回复 暮色英雄 的帖子4 L8 j) x+ _# T2 u$ w
5 m! O7 r% `# h
怎么还要注册网盘，太麻烦了5 c/ z% I6 s) N) j! J% E

wujing

看看咯

ndwzf

kankan

yuye3000

这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：）

暮色英雄

yuye3000 发表于 2012-3-1 23:01
: u& s# t( m1 m' {# Q这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：）

; Q# S, \1 Y3 H- F( [( T- ]$ f这个肯定是不能说的，我研究了很久才研究出来这个方法，如果告诉你了，那这个网站封锁了这个漏洞，以后就再也搞不到视频了。你可以把视频地址给我，然后我弄到视频后会在这里分享的。

icyfox_fox

谢谢分享

xiaf_0

谢谢分享