视频教程-人胶质母细胞瘤神经球培养方法

暮色英雄

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  P3 X. Y* \, j; d视频教程-人胶质母细胞瘤神经球培养方法
2 D8 \0 ^' C4 N* j" jVideo Article
' N9 q& |7 L+ s" F! b# l8 UIsolation and Expansion of Human Glioblastoma Multiforme Tumor Cells
) M! r8 R% ?& z2 d2 o6 cUsing the Neurosphere Assay* n/ K( T6 i3 S% H
Hassan Azari1,2, Sebastien Millette1, Saeed Ansari1, Maryam Rahman1, Loic P. Deleyrolle1, Brent A. Reynolds1
5 V2 g, |4 N" R& p1Department of Neurosurgery, University of Florida
; h( n/ P- p6 y3 x* K8 n, R0 Y2Department of Anatomical Sciences, Shiraz University of Medical Sciences
& O/ k: V+ ~+ O+ YCorrespondence to: Hassan Azari at Hassan.azari@neurosurgery.ufl.edu
6 Q& g1 k" e/ L: _2 KURL: http://www.jove.com/video/3633/
7 m/ q/ K' o5 l! I, W. oDOI: 10.3791/36335 g' y$ @0 B' |) C: \  r5 h; a2 O
Keywords: Neuroscience, Issue 56, Glioblastoma Multiforme, Tumor Cell, Neurosphere Assay, Isolation, Expansion,4 l) ?$ i! ?. Q. d
Date Published: 10/30/2011. B3 R& c& I/ c
Citation: Azari, H., Millette, S., Ansari, S., Rahman, M., Deleyrolle, L.P., Reynolds, B.A. Isolation and Expansion of Human Glioblastoma Multiforme. P4 o7 M0 _- t  v* v; f; q
Tumor Cells Using the Neurosphere Assay. J. Vis. Exp. (56), e3633, DOI : 10.3791/3633 (2011).
5 S  ^; t/ a+ r) MAbstract
5 n' H% l* [1 e9 O& YStem-like cells have been isolated in tumors such as breast, lung, colon, prostate and brain. A critical issue in all these tumors, especially in! P6 J! `: [4 o3 \; D
glioblastoma mutliforme (GBM), is to identify and isolate tumor initiating cell population(s) to investigate their role in tumor formation, progression,
1 `5 z; d6 D3 P! Q( Fand recurrence. Understanding tumor initiating cell populations will provide clues to finding effective therapeutic approaches for these tumors. The0 ~- b! m: x& y1 Y9 R2 ]
neurosphere assay (NSA) due to its simplicity and reproducibility has been used as the method of choice for isolation and propagation of many of7 B% Y# q& Q- P* }4 x. x8 q
this tumor cells. This protocol demonstrates the neurosphere culture method to isolate and expand stem-like cells in surgically resected human
) d, e2 ?. Z% k, L4 @GBM tumor tissue. The procedures include an initial chemical digestion and mechanical dissociation of tumor tissue, and subsequently plating the
; u% r2 _$ N9 q7 p7 zresulting single cell suspension in NSA culture. After 7-10 days, primary neurospheres of 150-200 μm in diameter can be observed and are ready
! w' F: z: e" B6 Efor further passaging and expansion.) ^) X: x; r/ `' X/ p; i
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下载地址：包含PDF和视频
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liuruii

谢谢楼主，先看看能不能下

leisong12

卡那看那

leisong12

回复 暮色英雄 的帖子
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怎么还要注册网盘，太麻烦了
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wujing

看看咯

ndwzf

kankan

yuye3000

这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：）

暮色英雄

yuye3000 发表于 2012-3-1 23:01   a% y& [! V* l# I
这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：）

6 V+ a9 [( b5 g1 w& \% K! ~
这个肯定是不能说的，我研究了很久才研究出来这个方法，如果告诉你了，那这个网站封锁了这个漏洞，以后就再也搞不到视频了。你可以把视频地址给我，然后我弄到视频后会在这里分享的。

icyfox_fox

谢谢分享

xiaf_0

谢谢分享