视频教程-人胶质母细胞瘤神经球培养方法

暮色英雄

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视频教程-人胶质母细胞瘤神经球培养方法9 o* @" v4 ?8 T' O, R  K7 `/ M" f4 _
Video Article7 _  Z3 k- i8 |* Q  j
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells
2 y7 H# k- V: PUsing the Neurosphere Assay8 _7 [% v  ~+ }8 M4 V" I
Hassan Azari1,2, Sebastien Millette1, Saeed Ansari1, Maryam Rahman1, Loic P. Deleyrolle1, Brent A. Reynolds1
. p1 m* `2 k; @& d- T1Department of Neurosurgery, University of Florida
! F( B) A5 A( V. G) N2Department of Anatomical Sciences, Shiraz University of Medical Sciences* ?) Y* j" x+ A
Correspondence to: Hassan Azari at Hassan.azari@neurosurgery.ufl.edu
! m2 ]! |( b& l$ U+ }3 X: @1 u5 n& wURL: http://www.jove.com/video/3633/0 |1 Z/ q6 w9 y
DOI: 10.3791/3633
# G2 Z0 L, I# N  `Keywords: Neuroscience, Issue 56, Glioblastoma Multiforme, Tumor Cell, Neurosphere Assay, Isolation, Expansion,6 F+ \6 y- e2 b8 m/ ^4 ^1 L
Date Published: 10/30/2011
( J0 s6 h$ K: sCitation: Azari, H., Millette, S., Ansari, S., Rahman, M., Deleyrolle, L.P., Reynolds, B.A. Isolation and Expansion of Human Glioblastoma Multiforme
4 p7 f" y# \5 Q, ]- ^5 STumor Cells Using the Neurosphere Assay. J. Vis. Exp. (56), e3633, DOI : 10.3791/3633 (2011).
; f& V/ f0 j4 s' hAbstract
& j: G. W5 e+ J7 k6 ~) E8 _* CStem-like cells have been isolated in tumors such as breast, lung, colon, prostate and brain. A critical issue in all these tumors, especially in' Z, o+ t1 h3 ^
glioblastoma mutliforme (GBM), is to identify and isolate tumor initiating cell population(s) to investigate their role in tumor formation, progression,* |. B- a+ v# `, \. u4 z
and recurrence. Understanding tumor initiating cell populations will provide clues to finding effective therapeutic approaches for these tumors. The
  v' U! A3 S: }# s- D7 b& L, ]neurosphere assay (NSA) due to its simplicity and reproducibility has been used as the method of choice for isolation and propagation of many of5 Y% A0 g, K+ N7 _
this tumor cells. This protocol demonstrates the neurosphere culture method to isolate and expand stem-like cells in surgically resected human
$ J- w! i+ p! n; @8 B  iGBM tumor tissue. The procedures include an initial chemical digestion and mechanical dissociation of tumor tissue, and subsequently plating the
9 b# W+ k  F. d  g3 M* \4 Gresulting single cell suspension in NSA culture. After 7-10 days, primary neurospheres of 150-200 μm in diameter can be observed and are ready
3 D- j$ S7 y8 L' e% @" F4 S4 G4 _for further passaging and expansion.
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3 e% _  U% P* \$ X& E* R! k/ ]下载地址：包含PDF和视频# X# h8 H' V7 `& D* d* W( |

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游客，如果你要查看本帖隐藏内容请回复

liuruii

谢谢楼主，先看看能不能下

leisong12

卡那看那

leisong12

回复 暮色英雄 的帖子
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怎么还要注册网盘，太麻烦了; i2 |. _8 C3 A+ s/ ^; J) G; {4 o) Q

wujing

看看咯

ndwzf

kankan

yuye3000

这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：）

暮色英雄

yuye3000 发表于 2012-3-1 23:01
5 A8 \5 f1 Y+ O9 @9 @4 C$ J5 d  @这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：）

3 c' j8 A: ^% K( L" p0 Q! g
这个肯定是不能说的，我研究了很久才研究出来这个方法，如果告诉你了，那这个网站封锁了这个漏洞，以后就再也搞不到视频了。你可以把视频地址给我，然后我弄到视频后会在这里分享的。

icyfox_fox

谢谢分享

xiaf_0

谢谢分享