视频教程-人胶质母细胞瘤神经球培养方法

暮色英雄

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视频教程-人胶质母细胞瘤神经球培养方法$ {) J6 C8 x0 k  N$ z
Video Article
- W1 c9 U0 G# GIsolation and Expansion of Human Glioblastoma Multiforme Tumor Cells
7 a6 Q2 l; l* t- h+ fUsing the Neurosphere Assay' G' x- I  R, G8 ~" f  G
Hassan Azari1,2, Sebastien Millette1, Saeed Ansari1, Maryam Rahman1, Loic P. Deleyrolle1, Brent A. Reynolds1/ g6 A: X, H0 [
1Department of Neurosurgery, University of Florida5 I6 [: P/ Z6 T. v1 \3 s
2Department of Anatomical Sciences, Shiraz University of Medical Sciences
! H: J  ], ]6 PCorrespondence to: Hassan Azari at Hassan.azari@neurosurgery.ufl.edu+ [  u4 h2 N3 x, Z  f4 D" `& t, S
URL: http://www.jove.com/video/3633/0 x8 {! h3 _6 c& T' i
DOI: 10.3791/3633" v2 i' _8 z; ~( \
Keywords: Neuroscience, Issue 56, Glioblastoma Multiforme, Tumor Cell, Neurosphere Assay, Isolation, Expansion,
; d  V, d* p* \Date Published: 10/30/2011
% p( J5 s0 F- O3 M; U$ gCitation: Azari, H., Millette, S., Ansari, S., Rahman, M., Deleyrolle, L.P., Reynolds, B.A. Isolation and Expansion of Human Glioblastoma Multiforme  i% C$ C7 v/ u1 S. n
Tumor Cells Using the Neurosphere Assay. J. Vis. Exp. (56), e3633, DOI : 10.3791/3633 (2011).5 r6 F( H, E& C" h
Abstract0 U: D" H! d% y2 L) D0 C1 ~
Stem-like cells have been isolated in tumors such as breast, lung, colon, prostate and brain. A critical issue in all these tumors, especially in- W7 \% o9 @  h0 q( Y0 `- t- ]
glioblastoma mutliforme (GBM), is to identify and isolate tumor initiating cell population(s) to investigate their role in tumor formation, progression,2 C8 Q- A0 O) f+ i
and recurrence. Understanding tumor initiating cell populations will provide clues to finding effective therapeutic approaches for these tumors. The
  a! |; m, {. d2 `& Q, cneurosphere assay (NSA) due to its simplicity and reproducibility has been used as the method of choice for isolation and propagation of many of0 x0 @& m# g/ Z
this tumor cells. This protocol demonstrates the neurosphere culture method to isolate and expand stem-like cells in surgically resected human* _' v9 F; x" Y7 K' R% \
GBM tumor tissue. The procedures include an initial chemical digestion and mechanical dissociation of tumor tissue, and subsequently plating the
" f0 ^2 N0 ^: S  Mresulting single cell suspension in NSA culture. After 7-10 days, primary neurospheres of 150-200 μm in diameter can be observed and are ready
1 R# F$ C5 n1 n. m8 |) Q3 O) k- Rfor further passaging and expansion.  F- p, V3 W1 n. \. k4 a2 f% K
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下载地址：包含PDF和视频: r( Z' }, [2 ~# ?* [
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游客，如果你要查看本帖隐藏内容请回复

liuruii

谢谢楼主，先看看能不能下

leisong12

卡那看那

leisong12

回复 暮色英雄 的帖子- H; V: H5 t0 r% }) Q
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怎么还要注册网盘，太麻烦了
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wujing

看看咯

ndwzf

kankan

yuye3000

这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：）

暮色英雄

yuye3000 发表于 2012-3-1 23:01 ) K& L/ ^. H; U2 a; c, S" Z
这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：）

+ P1 S% H* x. |0 v% y) ]6 e! z
这个肯定是不能说的，我研究了很久才研究出来这个方法，如果告诉你了，那这个网站封锁了这个漏洞，以后就再也搞不到视频了。你可以把视频地址给我，然后我弄到视频后会在这里分享的。

icyfox_fox

谢谢分享

xiaf_0

谢谢分享