视频教程-人胶质母细胞瘤神经球培养方法

暮色英雄

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- K% y% |% @# K视频教程-人胶质母细胞瘤神经球培养方法9 {4 J6 b) |2 v- U
Video Article
6 L! N9 P3 F* h7 x6 o2 C- F+ ~0 eIsolation and Expansion of Human Glioblastoma Multiforme Tumor Cells$ R' q. }0 I# |9 ]
Using the Neurosphere Assay* r: |% r; _5 N
Hassan Azari1,2, Sebastien Millette1, Saeed Ansari1, Maryam Rahman1, Loic P. Deleyrolle1, Brent A. Reynolds1
; k5 ^6 P/ L+ m# C+ T+ t1Department of Neurosurgery, University of Florida
  k* r+ |) L# X) e1 E! l0 e7 l2Department of Anatomical Sciences, Shiraz University of Medical Sciences
/ T: S2 z) X8 e) c+ X  O, {+ kCorrespondence to: Hassan Azari at Hassan.azari@neurosurgery.ufl.edu) r  z3 H4 o* y# u
URL: http://www.jove.com/video/3633/4 I3 Q. f0 ]# ~0 D: v3 w
DOI: 10.3791/3633
9 P) G+ N) g8 j& iKeywords: Neuroscience, Issue 56, Glioblastoma Multiforme, Tumor Cell, Neurosphere Assay, Isolation, Expansion,7 Z8 d8 N  Y! y+ k
Date Published: 10/30/2011
1 b; d4 A/ ?  ?! D; fCitation: Azari, H., Millette, S., Ansari, S., Rahman, M., Deleyrolle, L.P., Reynolds, B.A. Isolation and Expansion of Human Glioblastoma Multiforme- D3 m  H8 S, b6 \; N
Tumor Cells Using the Neurosphere Assay. J. Vis. Exp. (56), e3633, DOI : 10.3791/3633 (2011).; H, c, V7 t1 j6 x# d* \6 c5 |
Abstract
# O3 N4 {  ~3 x( l! [Stem-like cells have been isolated in tumors such as breast, lung, colon, prostate and brain. A critical issue in all these tumors, especially in
7 ^8 I' k+ j3 }/ Q3 g3 y2 cglioblastoma mutliforme (GBM), is to identify and isolate tumor initiating cell population(s) to investigate their role in tumor formation, progression," R  o0 f: r0 H4 y
and recurrence. Understanding tumor initiating cell populations will provide clues to finding effective therapeutic approaches for these tumors. The  x0 f8 I- N6 F  u3 a
neurosphere assay (NSA) due to its simplicity and reproducibility has been used as the method of choice for isolation and propagation of many of8 q0 ~& A* M9 \5 a
this tumor cells. This protocol demonstrates the neurosphere culture method to isolate and expand stem-like cells in surgically resected human
1 f$ G9 T. m4 b& GGBM tumor tissue. The procedures include an initial chemical digestion and mechanical dissociation of tumor tissue, and subsequently plating the
; o7 E- W  y, q6 E- j2 oresulting single cell suspension in NSA culture. After 7-10 days, primary neurospheres of 150-200 μm in diameter can be observed and are ready
/ a( R7 S- L7 A* c( l- E" {for further passaging and expansion.# t  f8 t6 ~- c1 Z
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下载地址：包含PDF和视频
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liuruii

谢谢楼主，先看看能不能下

leisong12

卡那看那

leisong12

回复 暮色英雄 的帖子
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怎么还要注册网盘，太麻烦了
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wujing

看看咯

ndwzf

kankan

yuye3000

这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：）

暮色英雄

yuye3000 发表于 2012-3-1 23:01
% _: z, G/ m( m0 m$ L( D  L0 ?这个很有用，不过这个网站要图书馆注册，不知你怎么弄到的。授人渔最好了：）

! X0 {! f4 H: r3 d这个肯定是不能说的，我研究了很久才研究出来这个方法，如果告诉你了，那这个网站封锁了这个漏洞，以后就再也搞不到视频了。你可以把视频地址给我，然后我弄到视频后会在这里分享的。

icyfox_fox

谢谢分享

xiaf_0

谢谢分享