cells began generating floating spheres, which were collected and plated in Petri dishes with growth factor medium. These spheres were cultured in ReNcell++ for at least 5 days and transferred to laminin-coated plates for monolayer outgrowth. / s1 d% b& u+ n8 {2 p; g* i看文献上的。不明白为什么把neurosphere先铺到petri dish,养几天后再转到laminin-coated plate呢? 9 u' }. m) Z# J1 T对petri dish不太了解,它不是一般培养细菌、或真核细胞的么?