关于干细胞染色体核型分析的一些问题

cartoonyang

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很好的帖子。
' E1 N# U; x; z/ w我之前做了很多体细胞的核型分析，分散效果一直不好。我在想，可能当初我没用预冷的载玻片有很大关系。
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cartoonyang

Briefly, actively proliferating cells were incubated with colchicines (0.1 lg/ml) for 4 h at 37 _C. The cells were washed twice with DPBS, trypsinized, suspended in a chilled hypotonic solution (68 mM KCl) and incubated for 20 min at 37 _C and then fixed for 10min in chilledfixative (methanol and glacial acetic acid, 3:1). The pellets were suspended in 5 ml of chilled fixative for another 10min. The metaphase spreads were prepared by dropping the cell suspension onto ice cold glass slides.
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网上找的，我准备用这个方法试试。供分享！