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本帖最后由 细胞海洋 于 2013-1-8 09:22 编辑 7 `; {: @( O2 I- ?' y1 ^
/ A5 {# L$ n; D8 b% n8 b( s
+ y$ R+ k6 z/ c# e- m+ M- q! O* Z FSpringer MMB Vol931 2013 共29章 552pages
8 O8 m" W2 C' ~, RCell Imaging Techniques Methods and Protocols/ `. R7 |, G5 X; z. b. e
Second Edition! v1 a' J9 m- T8 D5 ^. |7 v
Edited by
i4 O! J" u$ o0 m4 SDouglas J. Taatjes+ w r1 m; g" @3 ^, R/ ~
$ D, w( d0 t+ I K3 J/ \1 @
" ^" u% t4 a# ~- FContents
9 A- v' D, E0 b) ?! Z3 g5 Z4 v" m
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
& ^1 z1 I' ]/ B/ _5 OContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii+ S, s. M, A9 E& p+ M2 Z
1 Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . . 1+ a0 ?- a, I8 C6 V
Douglas W. Cromey5 N& e: d6 m" a. `5 r. C: F
2 Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29! g' a- ^3 V p R& ^% B
Donna J. Webb and Claire M. Brown
0 ?- [. e3 F: E- L0 r2 e3 Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . . . . . . . . . . . . . 61
: d! m* {$ ]6 ?1 b* ?4 ?* @- h8 t0 yJ. Lacoste, K. Young, and Claire M. Brown8 _# {8 i d6 H% P+ o
4 Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic 4 z* D: g# Z9 R) z- Z
Proteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85* P/ N; n7 Z- [4 C. ?3 Z ^# f
Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill
; B, K0 q8 y9 q) Y, A' r5 Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . 97
' y9 D0 k9 b! HJeremy Adler and Ingela Parmryd0 ~& z8 W5 Q4 H
6 A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . . 1116 ?% A: W* t3 V e
Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman
! I) M1 l- W5 U& m$ M2 q7 ?7 Light Sheet Microscopy in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
. _+ Q0 {1 y/ [9 mRaju Tomer, Khaled Khairy, and Philipp J. Keller( j: I& n5 l- n/ E) e
8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
3 Q8 V% K! }6 a0 \Lasse Evensen, Wolfgang Link, and James B. Lorens' R: I* x9 Z- t% q( A
9 Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . . 153$ Y. I# F0 W6 k: N9 U! u: ?; h
Andrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert
4 e* s5 Y6 [6 B" c6 I; d9 G3 {% F( X10 Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential
0 _. J9 Y' f0 ]* N; C! h$ P% s; @Interference
" B% s7 A+ }: l/ c1 y- LContrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . . 169& s- E0 A4 b/ d( G [
Wei Sun, Lehui Xiao, and Ning Fang7 s0 Y; g* A3 @3 J2 E
11 Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . . 187
+ `% Y$ G$ t6 `Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz
: X8 D* a- A, T) n3 L12 Laser Capture Microdissection for Protein" y2 U5 G1 I* _9 C; ?
and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213
/ u( O% T ]% P7 b1 lYelena Golubeva, Rosalba Salcedo, Claudius Mueller,: ?' Q6 O# a; L8 {
Lance A. Liotta, and Virginia Espina
& l2 ~+ z) l$ d5 p13 Viewing Dynamic Interactions of Proteins and a Model Lipid _' i( a* @' ~
Membrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . 2591 v, C3 B+ C! i v
Anthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes! L n. W5 ]* ?7 ?8 a
14 Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .295; x& p. y; X. @8 g- q j7 x
Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko3 w T( J$ s) N
15 Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313, Y/ U$ T1 u: Z( B. ?
Joanna L. MacKay and Sanjay Kumar
3 m, @5 Y- Z1 u16 Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331; O9 v2 Y! K. b: L
Lilia A. Chtcheglova and Peter Hinterdorfer
! ?7 S8 n1 V+ X17 Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . . 345
; j/ c4 z+ w4 u) W9 ^' Z" m& e% {Bhanu P. Jena! N6 E* V3 L! y W
18 Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . . 367
7 _5 `! u7 I4 E- E: z% S7 G) RChristian Mühlfeld, Lars Knudsen, and Matthias Ochs
- T' p' h$ h) ~; I19 A Novel Combined Imaging/Morphometrical Method for the Analysis
8 `3 y1 x m% f3 R: U- Q$ @of Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . . 391& Q) l/ j! a1 v* H% ^
Michele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes
( ]$ U4 A9 ~' \* W( \20 Correlative Light–Electron Microscopy as a Tool to Study
1 \6 f" I6 F& fIn Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . . 413
5 ]4 j. m' @9 j' d5 }5 |Elena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini f6 u$ @: l4 t1 i. \) |
21 Photooxidation Technology for Correlative Light8 b- h9 Q4 x7 `- s
and Electron Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 423* `- F2 m" B7 l# q' j4 q1 w
Claudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller,
2 V! u4 Q' m) z6 e$ M3 V+ qMargit Pavelka, and Adolf Ellinger0 s4 [: j9 Y* h1 C% s6 P
22 Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . . 4373 ?: L! I# x2 F2 {9 W S4 Z& ^
Carmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and : L% ^3 c9 f6 K3 ~: ?$ M& X
Margit Pavelka! S* c" `. D: K- W
23 Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 449
7 [0 S5 s# n0 d2 u9 R2 PKeisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori5 S; L' _8 S" @% M3 ]8 ]" V
24 Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . . 467, g" x3 i- ~3 }7 X" R
Nina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader/ T* J6 ~; s3 o; E. l' i. e
25 Histochemical Detection of Lipid Droplets in Cultured Cells . . . . . . . . . . . . . 483
. @! r5 @5 D- a' {Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto
7 U- N q8 g5 S. P26 Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . . 493
2 U# g. s/ S: w0 E" s% G1 BJ.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald" @; [: p# E$ P+ h3 D7 N9 T- Q5 {+ E$ S
27 Environmental Scanning Electron Microscopy Gold
6 T3 ^7 B! N2 l4 lImmunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 517
( j7 _5 C. ?; A" i/ x# s6 c8 f& rFrancesco Rosso, Ferdinando Papale, and Alfonso Barbarisi
7 d& o) K4 c4 r28 High-Pressure Freezing for Scanning Transmission Electron
3 g* P2 H* i/ o- h$ F3 H7 JTomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . . 525! s( D! w" `1 V" o/ o
Paul Walther, Eberhard Schmid, and Katharina Höhn
3 Y- c- }1 v. o' Z4 ?4 r29 MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . . 537
* F$ @7 F" n& m* mStephan Meding and Axel Walch* h0 R! E5 j5 Z& t; ?
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 547
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