PDF电子书：Cell Imaging Techniques (Springer MMB Vol931 2013)

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; a0 d9 e- u/ aSpringer MMB Vol931 2013 共29章 552pages
% K( X# H* h) y; I( SCell Imaging Techniques  Methods and Protocols
% }' w: K4 D4 ~+ C$ v. t8 RSecond Edition3 S4 _3 ~) A- R4 J
Edited by
! V# N. b, \2 e4 T& CDouglas J. Taatjes2 P0 s; [5 A2 [" z

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Contents$ z. t! c* x0 M. h

% r/ g2 M2 W; J- A( K: \! vPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      vii
2 A! k3 n) n  `9 KContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      xiii
6 B9 r6 P% c0 Z  n. I1  Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . .       1& m9 Z7 _4 ]' l# ?
Douglas W. Cromey- u- H- L# V* Q9 `
2   Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .       291 R' H3 C+ @; D4 e: `/ g' |
Donna J. Webb and Claire M. Brown
$ D- R+ E; a5 O3  Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . .     . . . . . . . . . . .   61
( g- a9 e+ O$ W$ ~5 DJ. Lacoste, K. Young, and Claire M. Brown. n! y% q1 C8 I/ M+ u" Q7 L& D# y
4  Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic
, t( n2 h3 |, A; D! wProteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 858 D; U2 O3 J& W: h+ J9 f
Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill$ P; [. ^  d& D- l
5   Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . .      97
* A, z3 H% F; D" d7 b4 }Jeremy Adler and Ingela Parmryd
% e" S% E5 v- N/ L6   A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . .    111
& A4 e# g! w- S2 ~/ U- u; E$ y/ OSunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman
1 ?8 R6 V/ t% I3 g0 r5 F1 G7   Light Sheet Microscopy in Cell Biology  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123, D& @( ^' a  O2 Z& t
Raju Tomer, Khaled Khairy, and Philipp J. Keller
4 f# C& Z# n7 I+ o0 u8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
& O; }5 O3 |7 G2 \/ C) J2 e3 {, `Lasse Evensen, Wolfgang Link, and James B. Lorens$ D& A7 f6 H1 s0 r7 I
9   Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . .     153$ I- F- {% }8 P2 q( X5 L
Andrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert
8 x4 g9 p  T- {2 U+ |. N* q  h" N10   Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential ( i1 Q- q; V( M0 g, J
Interference
7 i" m0 ]8 ^+ jContrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . .     169/ H0 Y6 P# m0 |3 a
Wei Sun, Lehui Xiao, and Ning Fang
7 `) {4 z5 R) o11   Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . .     187
1 F, [( `% o! E' H4 f( fPiotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz
& S* s# i/ v* b* |/ `5 h3 r0 @12   Laser Capture Microdissection for Protein
& A& G' `/ Z7 e. k6 ]. [and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2135 O4 M& C6 s  U- ]8 g9 A
Yelena Golubeva, Rosalba Salcedo, Claudius Mueller,
7 U6 [* s2 O9 uLance A. Liotta, and Virginia Espina7 g3 s4 ]' i0 ~7 x
13   Viewing Dynamic Interactions of Proteins and a Model Lipid
  ^% X4 f) |' w/ FMembrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . .     259
/ ], n3 O* r) P6 }. @Anthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes6 W- o# _. d2 U/ R3 h/ }  t
14   Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .295
8 @3 [1 m6 z! y' T8 C4 N0 [+ ]Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko
; t2 _* `3 H3 ^# Z; |+ w  T! j! ~15  Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313
% A7 ?- @# c( Y* U3 d$ _* B4 mJoanna L. MacKay and Sanjay Kumar! k% C* U, |2 R1 `" Y2 V
16   Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331
7 y5 w0 }+ [5 @( `- }Lilia A. Chtcheglova and Peter Hinterdorfer' d7 ^, u( T9 V- W$ u
17   Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . .     345
' ]' n7 F( Y7 {4 p0 A, iBhanu P. Jena
3 }# h* W; H# E18  Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . .    367; Y4 g: t% D5 j) x
Christian Mühlfeld, Lars Knudsen, and Matthias Ochs# L8 e5 e9 j. _+ T+ S$ \
19 A Novel Combined Imaging/Morphometrical Method for the Analysis
) I( E# G- l" |6 Kof Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . .    3911 G9 V- J& u8 ~, e8 u5 a  J
Michele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes
: Y' A! E  W7 p# f20   Correlative Light–Electron Microscopy as a Tool to Study
8 G6 L! E: L: v9 k" }2 \  E9 m) m% E  RIn Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . .    413+ x( w; i- K; u% H
Elena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini7 C: J% K% {' F/ u  l7 b
21   Photooxidation Technology for Correlative Light
  t4 Y4 ]8 G" b' b, M  r/ Nand Electron Microscopy  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     4238 D4 E/ \* e. o* \, Z7 }. Y4 {$ S6 v
Claudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller, 3 z& _  z1 R! t
Margit Pavelka, and Adolf Ellinger
% J( t& b3 e8 n) L, M7 s22   Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . .  437+ x- D& y2 Q* }2 }# G
Carmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and / u# c& ^' m! P) E: g0 z; R
Margit Pavelka
3 o$ i8 }6 R# K7 Q2 G2 k) d" _1 x23   Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     449
- b' D* }! Y3 K7 v2 N& H4 q$ QKeisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori5 q  m# v" b2 t0 Y- T6 A
24   Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . .     467
8 R) z. |9 _! y1 n; QNina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader+ h3 w) k8 ]* c( _" Y' a
25   Histochemical Detection of Lipid Droplets in Cultured Cells  . . . . . . . . . . . . .     483
. P. x& e7 z3 D0 a% GMichitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto
6 Y. u4 V3 ~  ?. P" M26  Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . .    493# J( F0 i% }- p. x- b0 @6 ]
J.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald9 W6 P! Y1 }' i; z- F% S* D6 y
27   Environmental Scanning Electron Microscopy Gold6 {+ c, [3 t2 q" O. N# W
Immunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .    517
, @8 m" C( I8 v8 tFrancesco Rosso, Ferdinando Papale, and Alfonso Barbarisi4 M5 W; Q  }/ n2 h, \5 e
28   High-Pressure Freezing for Scanning Transmission Electron& B, l+ R& ?0 j8 G
Tomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . .     525
7 C" {0 K! o- }' x! G) x5 {Paul Walther, Eberhard Schmid, and Katharina Höhn
# C. ^6 j& r' ]; G/ w5 A29  MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . .     537, x- q2 h# Y/ I# ~+ I
Stephan Meding and Axel Walch: c4 n3 e; Z: Z& U) ~7 s* f2 d+ X
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     547- e2 i3 f7 P3 }3 m& G
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Cell Imaging Techniques  Methods and Protocols% J, ^; `0 l) y; }8 ]5 S# x' A
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