PDF电子书：Cell Imaging Techniques (Springer MMB Vol931 2013)

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Springer MMB Vol931 2013 共29章 552pages
6 B6 Y( g7 z$ ~: V; XCell Imaging Techniques  Methods and Protocols
3 x+ ^3 l$ l0 f6 @, `Second Edition
+ _; @# u& Y& |$ d( \0 ?Edited by- D+ Q" _# P" g! ^5 T
Douglas J. Taatjes3 |: w& V8 V0 S4 F+ O. ^

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1 q# ^8 [3 v# N1 x+ IContents
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Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      vii, @# B  S# h0 G! S
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .      xiii5 t' ]/ t8 P( Q8 m7 D9 ?8 `
1  Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . .       13 F% x/ n4 Q' O  ^
Douglas W. Cromey% d& y5 z. R& i! }: M
2   Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .       291 ]' n* k8 h( c3 X
Donna J. Webb and Claire M. Brown- w* Z) U& y: I
3  Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . .     . . . . . . . . . . .   61# F) v1 w  C( T) {$ l9 t
J. Lacoste, K. Young, and Claire M. Brown* @- f8 N+ P: C9 |" Z
4  Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic ! `8 ]- c7 ~/ w1 u
Proteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
8 Z. _( W& g4 b# e$ n/ \0 ]Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill8 E. m" L% ~$ D% T9 C) Z' ?  b8 d0 y
5   Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . .      97
% l5 Y* w7 a! ]9 f1 j8 z9 NJeremy Adler and Ingela Parmryd& b4 b  F% b" e' Z4 S* k$ C% Y  l
6   A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . .    111
  }1 }9 w: W1 e1 N* hSunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman
, m8 {8 l! F1 `9 c' I: n7   Light Sheet Microscopy in Cell Biology  . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123! r/ V" |7 d/ H. c9 u, B9 o' @
Raju Tomer, Khaled Khairy, and Philipp J. Keller
8 g' N/ K4 [6 L% u/ y+ ?8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139% ^) Z8 w0 a5 c' R0 _
Lasse Evensen, Wolfgang Link, and James B. Lorens
4 Z% I. G! P8 i, Q  Z2 @9   Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . .     153
& G4 C% H* N' i: `7 A0 U. N9 V- HAndrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert
/ O# l8 @/ J, K2 g" j3 a10   Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential / A9 P& K1 E) o) Q8 N' H
Interference# L& _0 Q3 |# A4 H0 K3 O0 K
Contrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . .     169
6 `% Z% f  [& k) M0 KWei Sun, Lehui Xiao, and Ning Fang
$ M$ U# a0 a" f) u  Z: F" A11   Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . .     187
: `. y  h) }/ t6 F5 c& O9 gPiotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz
. ~: a  e, Z0 `( [12   Laser Capture Microdissection for Protein% i  _  n" ?$ E& j5 G1 ^1 ]7 k
and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213. _+ H( k! C- V2 t
Yelena Golubeva, Rosalba Salcedo, Claudius Mueller,
! j: i! N: w- I  l7 kLance A. Liotta, and Virginia Espina
, p+ L! i0 r9 A) z! }2 E7 q13   Viewing Dynamic Interactions of Proteins and a Model Lipid3 R1 _% J5 s; b5 O* v
Membrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . .     259
/ ?* Q3 G( x$ k/ V0 qAnthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes
3 W; s: T) ?: D14   Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .295, n/ Y" P( X( E9 X3 h
Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko
& \2 i4 |1 q. `15  Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .3139 u! [, f( a1 ^/ U
Joanna L. MacKay and Sanjay Kumar( M1 U( ~5 t2 h' Z) U
16   Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331- M- b& T0 j" E7 H7 K
Lilia A. Chtcheglova and Peter Hinterdorfer
2 `) v6 A4 N/ r# e6 U0 g8 ~. p17   Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . .     345* u8 [* p. g/ M; Q0 B3 k7 e0 P0 n
Bhanu P. Jena
  d+ u9 t0 @( k; R9 E1 }18  Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . .    367
' P4 O- E# F: E+ W' XChristian Mühlfeld, Lars Knudsen, and Matthias Ochs
4 I8 y( k( n) Z3 F! `19 A Novel Combined Imaging/Morphometrical Method for the Analysis
. l8 u4 f+ Y, K5 v% A: Zof Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . .    391
( ~% n! T+ I0 u# Y3 s, eMichele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes
+ ]) s+ u# }# M' l" t. t! J20   Correlative Light–Electron Microscopy as a Tool to Study
+ ?( b6 E0 {8 N/ `/ HIn Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . .    413
2 S1 w! b) t6 ^1 t# `( {" qElena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini
3 Y% z+ t* r7 B# L21   Photooxidation Technology for Correlative Light
. W) ]9 Z( L( E4 O6 pand Electron Microscopy  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     423- e) \. @) a$ e: }: }" e
Claudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller, 4 E% Y4 [/ Z$ |+ |( M
Margit Pavelka, and Adolf Ellinger7 x; q  F. i, k' K
22   Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . .  437
( n& e; ~# Y. p7 BCarmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and 7 }8 e! d$ a$ x" k
Margit Pavelka0 M' p0 C6 b4 z; W) ]1 C  e) ~2 b
23   Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     449
/ X0 S: i, O! ^  `2 j) b8 X! HKeisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori
. L3 U3 z- ]# E) n24   Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . .     467
4 q2 C1 M- f+ U3 p, _) q8 a4 m# jNina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader
/ O& k: W/ p' z! I) m25   Histochemical Detection of Lipid Droplets in Cultured Cells  . . . . . . . . . . . . .     4834 c, M0 F4 l& P* a6 ]; f3 T6 U
Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto
, [5 a, R: j+ k, d4 ^/ o26  Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . .    4938 c% q" `+ i) o% o5 J
J.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald( S) ?* Z( p! L
27   Environmental Scanning Electron Microscopy Gold: ]$ @4 V$ L2 Q5 l# n0 Y& M
Immunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .    517
5 E! p1 t5 l) f) K+ [2 H6 H- L. GFrancesco Rosso, Ferdinando Papale, and Alfonso Barbarisi
) Q; F, b0 R' b* h, z) I& y28   High-Pressure Freezing for Scanning Transmission Electron8 u/ @5 j$ h: z# N; I
Tomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . .     525; u4 H& V% _9 I! ~- [
Paul Walther, Eberhard Schmid, and Katharina Höhn
, ^" K5 s. j0 t3 Q! w% x29  MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . .     537$ R: i% x+ U% M6 ?
Stephan Meding and Axel Walch
' n2 w4 {0 |' \; ]# ]  jIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     547
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Cell Imaging Techniques  Methods and Protocols
5 A0 o9 j9 e+ n! HSecond Edition

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