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本帖最后由 细胞海洋 于 2013-1-8 09:22 编辑 * M7 v; t1 y1 m6 h8 r6 j+ {4 v
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Springer MMB Vol931 2013 共29章 552pages
9 h, V) O4 d0 n( d' e5 e' p8 jCell Imaging Techniques Methods and Protocols$ d' l6 Q5 y* W! ]3 q9 X$ L' k
Second Edition' W$ n9 t! M6 N5 l% h' k
Edited by
& E2 _6 Q7 t2 d7 j( TDouglas J. Taatjes5 M2 o- J: M6 e6 M3 s7 D
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0 {$ T5 M" h( r: ?( m( w# W% S# IContents& K% E( I3 h( ^
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Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii. U9 T. h% B" ?, A( ~* b$ R, p
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
0 u; q# ]* Z5 k7 |. Y1 Digital Images Are Data: And Should Be Treated as Such . . . . . . . . . . . . . . . . 1# r9 {$ i3 j) D, ~
Douglas W. Cromey
/ J3 `" T4 n$ v; ]1 ]8 ^3 z2 Epi-Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 290 \7 _' n4 k) v4 g" O
Donna J. Webb and Claire M. Brown
" h1 S2 t3 U( \3 Live-Cell Migration and Adhesion Turnover Assays . . . . . . . . . . . . . . . . . . . . 61; [$ `0 G" P9 q' Y, m) |5 @8 E
J. Lacoste, K. Young, and Claire M. Brown4 E5 `1 N K8 p6 u& M
4 Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic
6 P1 J8 F, L% @! tProteins in Human Venous Valves. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
0 c3 W* ^- N% \8 MWinifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill/ I3 v3 o2 T% b9 i1 F. V
5 Colocalization Analysis in Fluorescence Microscopy . . . . . . . . . . . . . . . . . . . . 97
( n' t4 r+ f& h1 W- hJeremy Adler and Ingela Parmryd5 O& J2 f K) k: D8 e& b1 {
6 A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown . . . . . . . 111
7 ]& F( ^- K0 m! hSunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman" W+ P: P |& {# T
7 Light Sheet Microscopy in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123. k6 p7 _) \" Z( ^. s1 Y
Raju Tomer, Khaled Khairy, and Philipp J. Keller
# H( V; ?+ [, ~% L* s8 k/ F# t% _. V8 Image-Based High-Throughput Screening for Inhibitors of Angiogenesis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1391 P/ k% m( C$ C0 l8 a9 f: x
Lasse Evensen, Wolfgang Link, and James B. Lorens9 F# a5 O* b! z$ O# D" D
9 Intravital Microscopy to Image Membrane Trafficking in Live Rats . . . . . . . . . 153
4 Q, m* p; g2 R1 q p9 s( C6 L! eAndrius Masedunskas, Monika Sramkova, Laura Parente, and Roberto Weigert
3 T9 h& V8 C! K: g8 x8 [! d10 Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential ' b7 A. m5 N- S
Interference+ r+ J8 ]1 A, D( L
Contrast Microscopy and Planar Illumination Microscopy . . . . . . . . . . . . . . . . 1691 s/ E- T8 s( R2 F) w( c
Wei Sun, Lehui Xiao, and Ning Fang
$ l' ?) B& w" A. a2 K: c+ C+ I11 Laser Scanning Cytometry: Principles and Applications—An Update . . . . . . . . 1878 d! a4 E( D) F. v/ P
Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz3 c. Y4 c' }' Y( P }
12 Laser Capture Microdissection for Protein4 w/ G1 s" g, g z/ D
and NanoString RNA Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213" J% i( ~# y, z; l* @
Yelena Golubeva, Rosalba Salcedo, Claudius Mueller,
4 G* s% t* s {3 nLance A. Liotta, and Virginia Espina7 {5 o, h9 d1 ` h
13 Viewing Dynamic Interactions of Proteins and a Model Lipid
4 a; ]1 N8 E: h0 pMembrane with Atomic Force Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
8 G, l; u% @: P# o% I0 Z" i9 EAnthony S. Quinn, Jacob H. Rand, Xiao-Xuan Wu, and Douglas J. Taatjes
- d% J3 F3 u: p; \14 Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy . . . . . . . . . . . . .295
- F1 X* Q' _# p/ y; x$ zLuda S. Shlyakhtenko, Alexander A. Gall, and Yuri L. Lyubchenko2 W- p% {. o( Y! [9 G W
15 Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation. . . . . . . . . . . . . . . . . . . . . . . .313
( \! _6 T: l- s$ OJoanna L. MacKay and Sanjay Kumar
: U- k% B$ _1 s. X/ g3 Y16 Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3316 g1 V- Z" C7 c! w9 P" |+ n7 [4 m
Lilia A. Chtcheglova and Peter Hinterdorfer
6 g0 S7 [4 }( t( }0 [3 h# @- {17 Porosome: The Secretory NanoMachine in Cells . . . . . . . . . . . . . . . . . . . . . . . 3451 u) C- o' r$ s0 _2 C7 f8 z. P
Bhanu P. Jena
5 k6 I8 V; G3 R& G18 Stereology and Morphometry of Lung Tissue . . . . . . . . . . . . . . . . . . . . . . . . . 3678 R& j- s% n' v& V. b
Christian Mühlfeld, Lars Knudsen, and Matthias Ochs
4 a; o* I( u$ [. o, A19 A Novel Combined Imaging/Morphometrical Method for the Analysis! S/ j& B e* g4 I+ v! }; a
of Human Sural Nerve Biopsies for Clinical Diagnosis . . . . . . . . . . . . . . . . . . . 3913 m9 D& V- v- o8 X! N
Michele A. vonTurkovich, Marilyn P. Wadsworth, William W. Pendlebury, and Douglas J. Taatjes
& y* y+ D0 ?2 _2 ]8 E6 V20 Correlative Light–Electron Microscopy as a Tool to Study' S/ u; }2 g" [, L
In Vivo Dynamics and Ultrastructure of Intracellular Structures . . . . . . . . . . . 413
& y8 T# e- N- }& kElena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini
9 ]) O8 N. w# Y, {& f& t- v/ W: y" R21 Photooxidation Technology for Correlative Light- w& @5 h* m$ P u% G
and Electron Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4236 h% X4 s" G4 E$ w5 |8 a
Claudia Meisslitzer-Ruppitsch, Clemens Röhrl, Carmen Ranftler, Herbert Stangl, Josef Neumüller, 4 N% r7 u1 q# a, u+ M" z
Margit Pavelka, and Adolf Ellinger! ~9 K/ }' g7 N" i6 W# h2 Q
22 Electron Microscopy of Endocytic Pathways . . . . . . . . . . . . . . . . . . . . . . . . . . 437* g8 N9 w T7 R1 G
Carmen Ranftler, Peter Auinger, Claudia Meisslitzer-Ruppitsch, Adolf Ellinger, Josef Neumüller, and 5 L# L1 x& R$ F! ^
Margit Pavelka. H" @+ c% G4 s9 G+ m- q) N+ }
23 Morphological Analysis of Autophagy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 449
3 _- e4 t; i' K' M: ?Keisuke Tabata, Mitsuko Hayashi-Nishino, Takeshi Noda, Akitsugu Yamamoto, and Tamotsu Yoshimori' ~8 [* q/ g2 q) d" V V
24 Cytochemical Detection of Peroxisomes and Mitochondria . . . . . . . . . . . . . . . 467
+ B4 Z2 b" S$ F1 D" G+ KNina A. Bonekamp, Markus Islinger, Maria Gómez Lázaro, and Michael Schrader
5 Z% d! z3 h% s' t+ T) ~25 Histochemical Detection of Lipid Droplets in Cultured Cells . . . . . . . . . . . . . 4834 Q9 `' q6 G3 h* ~1 K
Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto0 h7 R& x1 L1 k1 M' |0 L
26 Environmental Scanning Electron Microscopy in Cell Biology. . . . . . . . . . . . . 493
" K5 O* W7 _. oJ.E. McGregor, L.T.L. Staniewicz, S.E. Guthrie (neé Kirk), and A.M. Donald
2 O, `4 o( M9 V- B B T+ q% u27 Environmental Scanning Electron Microscopy Gold) e- ]4 z! v& m _9 l% c3 I- G
Immunolabeling in Cell Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 517* L8 [6 j w1 b! J- U; k
Francesco Rosso, Ferdinando Papale, and Alfonso Barbarisi
& U/ G( J- y) v4 t# z$ o1 r28 High-Pressure Freezing for Scanning Transmission Electron' C+ v" w- y3 v* D/ ^: \
Tomography Analysis of Cellular Organelles . . . . . . . . . . . . . . . . . . . . . . . . . . 5250 `5 g2 W/ h5 m& r
Paul Walther, Eberhard Schmid, and Katharina Höhn
# }) K: k& V F' ? ~+ x29 MALDI Imaging Mass Spectrometry for Direct Tissue Analysis. . . . . . . . . . . . 537
& c! h, I4 o9 A2 b1 ^, o. D# a) VStephan Meding and Axel Walch
% Y( ~3 _& Z' ?: BIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 547
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