PDF电子书：Therapeutic Oligonucleotides (Springer MMB-V764, 2011).pdf

xyklmu

% x' a2 r' `3 b) d9 E! x
: S' e+ K, m, L) t[hide][/hide]
5 [1 ~- Z: r' J/ x8 E3 eTherapeutic Oligonucleotides
4 I$ `2 Y* ^) O4 BMethods and Protocols   Springer MMB-V764, 2011     
# T" G3 A' n, P9 O. j) g5 ]+ zEdited by
" b0 a5 e  y- c- SJohn Goodchild
1 Z/ P6 p6 d1 fDepartment of Chemistry, Worcester State University,Worcester, MA, USA
* [4 r4 m) o3 F# N! M: b' B9 j) t4 ?, }( x5 _
Contents
$ T. {7 N# h8 c+ y  T* S2 OPreface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v- ]/ @! N) d, s, R
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix& t9 H- L% p9 l2 e: s
1. Therapeutic Oligonucleotides . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
9 x5 F4 A6 g5 `; v; z  {. BJohn Goodchild4 w9 g/ u8 v# v
2. Dinucleotides Containing 3-S-Phosphorothiolate Linkages . . . . . . . . . . . . 17
. u9 _3 R& Q3 C' B- \: ]( |  ]James W. Gaynor and Richard Cosstick6 q) Z* C6 m# y3 k1 X" \7 _- P
3. 2-O,4-C-Methyleneoxymethylene Bridged Nucleic Acids (2,4-BNACOC) . . . . 31# i* \4 v) C8 z, t/ j; P$ p; S* \
Yoshiyuki Hari, Tetsuya Kodama, Takeshi Imanishi,) P3 t; s6 X4 E* E( g7 _1 h8 C8 O
and Satoshi Obika7 {% c* F0 B0 Q$ r& N
4. A Non-covalent Peptide-Based Strategy for Ex Vivo and In Vivo
7 H8 q/ A! m9 ?8 B8 TOligonucleotide Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
- {* h1 s9 y0 WLaurence Crombez, May C. Morris, Frederic Heitz, and Gilles Divita2 s. {: ^$ |' Q# E% M$ c  [
5. Cell-Penetrating Peptides-Based Strategies for the Delivery of Splice
3 w* h! _6 ^3 P/ y( iRedirecting Antisense Oligonucleotides . . . . . . . . . . . . . . . . . . . . . . 75! V; l" U* Q. R
Samir El Andaloussi, Fatouma Said Hassane, Prisca Boisguerin,% z; p2 u, Z0 N9 z3 ^: _
Rannar Sillard, Ülo Langel, and Bernard Lebleu2 C- L. g  u4 K3 {
6. A Nanoparticle for Tumor Targeted Delivery of Oligomers . . . . . . . . . . . . 91
* p# [0 Q0 q4 s. }$ LXinrong Liu, Yi Wang, and Donald J. Hnatowich1 j2 B9 R9 |. x  @
7. Light-Directed Delivery of Nucleic Acids . . . . . . . . . . . . . . . . . . . . . 107: B4 o% ?# Y8 f& f# T
Sigurd Bøe, Lina Prasmickaite, Birgit Engesæter, and Eivind Hovig
5 P, ?: o, n, {! X8. Antibody Targeted siRNA Delivery . . . . . . . . . . . . . . . . . . . . . . . . 123* G! V2 F+ U1 p* k& [+ w0 y1 Q
Masoud M. Toloue and Lance P. Ford: R) Z7 x* }% p% ?# m2 c: c5 K# ?' K
9. Aptamer–Drug Conjugation for Targeted Tumor Cell Therapy . . . . . . . . . . 141
8 T& G; [! o6 q: q0 W& `Michael J. Donovan, Ling Meng, Tao Chen, Yunfei Zhang,
/ c6 p' ~  S" WKwame Sefah, and Weihong Tan
& x4 D0 R8 g4 f$ C10. Five-Step Process for Screening Antisense Compounds for Efficacy: Gene
2 W" H7 V6 t: k9 h7 }' OTarget IL-12Rb2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153
6 b( E' S' `* F  @$ yNikki B. Marshall, Laura L. Hauck, and Dan V. Mourich3 P& l( z3 K, j# h- k
11. Diverse Small Non-coding RNAs in RNA Interference Pathways . . . . . . . . . 169- X8 k7 T- Y' T! Y! _
Liande Li and Yi Liu+ n- R# P! \1 k) e! v$ [$ D
12. Quantification of siRNAs In Vitro and In Vivo . . . . . . . . . . . . . . . . . . . 183
0 e5 d, q: D) g2 eAngie Cheng, Alexander V. Vlassov, and Susan Magdaleno5 _: j& X1 k7 b
vii
/ V) B; T$ D: G: v( pviii Contents
6 F9 u6 v0 D2 z' A0 O- l* R$ l. ~13. Optimization of Transfection Conditions and Analysis of siRNA Potency  X8 b" f1 _7 F# v! H: n
Using Real-time PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1993 q) z# v# i% c/ d
Angie Cheng, Susan Magdaleno, and Alexander V. Vlassov
  {" W( E* g+ n- N& C) w14. siRNA Knockdown of Gene Expression in Endothelial Cells . . . . . . . . . . . . 215* z; w- `% p. A. q* K) Y; W7 A3 m
Emily Dennstedt and Brad Bryan
: T; F2 Z' Q* ~15. Using RNA Interference in Schistosoma mansoni . . . . . . . . . . . . . . . . . . 2237 v, a8 i; H. ?7 L
Rita Bhardwaj, Greice Krautz-Peterson, and Patrick J. Skelly7 Q& _% N+ c, s7 c
16. Performing the Labeled microRNA Pull-Down (LAMP) Assay System:
! S1 i  \) n! R5 y( FAn Experimental Approach for High-Throughput Identification of- f) E) I- r* c8 K% O  u9 r. W
microRNA-Target mRNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 241
# K6 M5 m" j! I3 ZRen-Jun Hsu and Huai-Jen Tsai
5 X( S9 P& y' y8 C  Q# y1 [8 }% T17. Synthesis, Purification, and Characterization of Oligoribonucleotides that
- ?/ ?: t$ G9 [$ s" ]6 D& hAct as Agonists of TLR7 and/or TLR8 . . . . . . . . . . . . . . . . . . . . . . 249; n! }/ u4 d4 L
Tao Lan and Ekambar R. Kandimalla* G' s3 D5 V( @3 H) K
18. Synthesis, Purification, and Characterization of Immune-Modulatory# j" Y! D. |8 E* T3 }4 F2 {& b
Oligodeoxynucleotides that Act as Agonists of Toll-Like Receptor 9 . . . . . . . . 263
# b8 E6 H! I( L! jMallikarjuna Reddy Putta, Dong Yu, and Ekambar R. Kandimalla
; k: I. }, @4 z" z* S19. Surface Plasmon Resonance Investigation of RNA Aptamer–RNA Ligand
; _. R, a* h& e+ dInteractions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279) O  _; `9 E8 ?/ Q# B- G) u% A! h( G6 c
Carmelo Di Primo, Eric Dausse, and Jean-Jacques Toulmé
8 W, g0 S  C! M. v20. Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA
* l: G) U- D6 I# V# [" D- M9 OImmunoprecipitation of Argonaute Protein . . . . . . . . . . . . . . . . . . . . 3019 Z" Q9 {; b% N! Z
Jacob C. Schwartz and David R. Corey7 a; F1 r$ m3 Q( k5 Z2 Z
21. Inhibition of Human Papillomavirus Expression Using DNAzymes . . . . . . . . 317
* R' B- J1 @) V" L$ A- ?/ PMaría Luisa Benítez-Hess, Pablo Reyes-Gutiérrez,! u# K; V( I5 t% H9 U
and Luis Marat Alvarez-Salas3 U* G1 D3 ^* E/ C
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 337

hualin

ziyumo

nice

bluesuns

呵呵 高高实在是高~~~~~  

dypnr

谢谢分享了!  

sky蓝

勤奋真能造就财富吗？  

foxok

慢慢来，呵呵  

石头111

呵呵 大家好奇嘛 来观看下~~~~  

罗马星空

我起来了 哈哈 刚才迷了会  

科研人

我的啦嘿嘿