PDF电子书：Therapeutic Oligonucleotides (Springer MMB-V764, 2011).pdf

xyklmu

4 i( q3 Y6 K! v( D/ A
' X5 W# m" n. Y8 I$ [0 e9 G7 Z; g[hide][/hide]
: E5 Y: r/ Y1 F! J# z& STherapeutic Oligonucleotides
1 e0 A/ Y! K' u3 [/ S$ U5 zMethods and Protocols   Springer MMB-V764, 2011     " v) Z; b5 k5 H3 t
Edited by8 M* {- p( w8 g- b: M
John Goodchild
) o: u4 m5 b9 k+ d" e, ]) k) i+ {Department of Chemistry, Worcester State University,Worcester, MA, USA
/ }; }. Z* x) E. L  X% l" S  k0 X' A" t" g1 h
Contents7 U& A+ z' Z1 [* K! [
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
; C7 F* [0 Y8 o  @/ e- P, ]Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
* Q5 u6 P- L; B! Z1. Therapeutic Oligonucleotides . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
5 Q9 M$ f& i8 Y1 h3 V% r+ Q8 {' lJohn Goodchild( d& q0 q1 N# o! v. H  A, z' ]5 Z
2. Dinucleotides Containing 3-S-Phosphorothiolate Linkages . . . . . . . . . . . . 17
0 X6 U( W6 K7 V5 f) n% y5 NJames W. Gaynor and Richard Cosstick
7 x* b. N" N& t# f- c" L. B3. 2-O,4-C-Methyleneoxymethylene Bridged Nucleic Acids (2,4-BNACOC) . . . . 31/ x+ o5 {- C% a, H; ?+ `" N
Yoshiyuki Hari, Tetsuya Kodama, Takeshi Imanishi,
9 ]9 O; P  T- c7 t/ F+ Xand Satoshi Obika
- T5 j* @3 B3 u+ ]# y4. A Non-covalent Peptide-Based Strategy for Ex Vivo and In Vivo! G# R4 y! g) L3 j3 M
Oligonucleotide Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59' H4 b( H: [& D% x  g$ }# |( A
Laurence Crombez, May C. Morris, Frederic Heitz, and Gilles Divita
4 H+ R; n5 l0 o5. Cell-Penetrating Peptides-Based Strategies for the Delivery of Splice7 }7 H3 M2 X# m7 i  ?
Redirecting Antisense Oligonucleotides . . . . . . . . . . . . . . . . . . . . . . 75; Z$ k+ m& I& c: m
Samir El Andaloussi, Fatouma Said Hassane, Prisca Boisguerin,  D6 r6 w- @. U8 _' Q7 A) M) {5 n
Rannar Sillard, Ülo Langel, and Bernard Lebleu
' Z  l7 E% l- l  D" K) L6. A Nanoparticle for Tumor Targeted Delivery of Oligomers . . . . . . . . . . . . 91
$ b8 l8 v7 I# b# z9 K+ G: M# UXinrong Liu, Yi Wang, and Donald J. Hnatowich
! W# W4 d2 q  z7 l7 l7. Light-Directed Delivery of Nucleic Acids . . . . . . . . . . . . . . . . . . . . . 107: r1 p- }7 \9 ~6 x' ]% q
Sigurd Bøe, Lina Prasmickaite, Birgit Engesæter, and Eivind Hovig* R: k& r' q: O7 P0 x
8. Antibody Targeted siRNA Delivery . . . . . . . . . . . . . . . . . . . . . . . . 1234 w2 p/ ]! A2 ~& Y
Masoud M. Toloue and Lance P. Ford
" c4 S/ A+ D" i; ]8 f9. Aptamer–Drug Conjugation for Targeted Tumor Cell Therapy . . . . . . . . . . 141
* N) g% y3 E6 k' U! @Michael J. Donovan, Ling Meng, Tao Chen, Yunfei Zhang,
! E% k; _( O# }( ?% \2 a5 DKwame Sefah, and Weihong Tan
) e! z( L; g" j" F: h10. Five-Step Process for Screening Antisense Compounds for Efficacy: Gene
% L) X5 f* |. P1 t; e3 j( ITarget IL-12Rb2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153
3 s, `6 {% f- `- a3 O7 BNikki B. Marshall, Laura L. Hauck, and Dan V. Mourich, N( Y  m! i8 U: @3 V  y
11. Diverse Small Non-coding RNAs in RNA Interference Pathways . . . . . . . . . 169) o, F# U& T4 s- O* J3 k
Liande Li and Yi Liu6 P) x5 \0 ?9 y1 M% _, n
12. Quantification of siRNAs In Vitro and In Vivo . . . . . . . . . . . . . . . . . . . 1838 {7 n4 E/ N: x
Angie Cheng, Alexander V. Vlassov, and Susan Magdaleno& w* r5 o+ V& a  P
vii
9 k# M8 W7 `2 q0 w+ H6 fviii Contents  l) o% L4 r8 J7 V" Q- r# ?7 s* E
13. Optimization of Transfection Conditions and Analysis of siRNA Potency( d# F8 ~/ k) N$ A% u5 Y' Q& A
Using Real-time PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 199+ j+ E, F6 u, N9 R1 D( z
Angie Cheng, Susan Magdaleno, and Alexander V. Vlassov
) B8 [: z9 T+ \+ w# Y, Y14. siRNA Knockdown of Gene Expression in Endothelial Cells . . . . . . . . . . . . 215( m) y- g; Y% q: P( o) h% n3 U
Emily Dennstedt and Brad Bryan9 N+ @8 k: ?1 g2 s6 a3 r# l
15. Using RNA Interference in Schistosoma mansoni . . . . . . . . . . . . . . . . . . 223# Z+ {' G! Q, p& M( B
Rita Bhardwaj, Greice Krautz-Peterson, and Patrick J. Skelly
: y8 B# A  ?5 u16. Performing the Labeled microRNA Pull-Down (LAMP) Assay System:0 {: u* {; a+ a
An Experimental Approach for High-Throughput Identification of% l; z# S8 ~& t2 E
microRNA-Target mRNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 241' C9 @, D" K: s  _' }9 {
Ren-Jun Hsu and Huai-Jen Tsai
, h" Y* D9 u: P2 z4 X7 @( @17. Synthesis, Purification, and Characterization of Oligoribonucleotides that2 z" s; {, d5 ?2 a1 u
Act as Agonists of TLR7 and/or TLR8 . . . . . . . . . . . . . . . . . . . . . . 249) B$ }2 t6 Q5 X
Tao Lan and Ekambar R. Kandimalla
. T+ Z* \/ c0 {* w- T) f9 U& v) |18. Synthesis, Purification, and Characterization of Immune-Modulatory, s/ a/ `" t8 T
Oligodeoxynucleotides that Act as Agonists of Toll-Like Receptor 9 . . . . . . . . 2637 c8 a; M3 _  h& D
Mallikarjuna Reddy Putta, Dong Yu, and Ekambar R. Kandimalla! v( T( K3 Z1 V7 ]( L
19. Surface Plasmon Resonance Investigation of RNA Aptamer–RNA Ligand6 r% v7 X0 q1 I3 v' g. `8 v
Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279" U' e+ ~, H7 X5 v: u. n  ]+ ]) e
Carmelo Di Primo, Eric Dausse, and Jean-Jacques Toulmé
  R( h' o) y! Z0 _20. Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA
6 o4 G; P7 r/ T6 K1 Q6 _, ^Immunoprecipitation of Argonaute Protein . . . . . . . . . . . . . . . . . . . . 301  N! C8 w1 z& \# G
Jacob C. Schwartz and David R. Corey
6 n, U5 H& K8 c7 M; l# T21. Inhibition of Human Papillomavirus Expression Using DNAzymes . . . . . . . . 317
9 x7 S' ?, R8 j) RMaría Luisa Benítez-Hess, Pablo Reyes-Gutiérrez,
( d& N; X+ P! ?# F6 u( iand Luis Marat Alvarez-Salas, A# c" |- ]. z' o+ k% e1 l% w$ E5 n
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 337

hualin

ziyumo

nice

bluesuns

呵呵 高高实在是高~~~~~  

dypnr

谢谢分享了!  

sky蓝

勤奋真能造就财富吗？  

foxok

慢慢来，呵呵  

石头111

呵呵 大家好奇嘛 来观看下~~~~  

罗马星空

我起来了 哈哈 刚才迷了会  

科研人

我的啦嘿嘿