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Reprogrammed by a frog [复制链接]

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发表于 2009-3-6 00:48 |只看该作者 |正序浏览 |打印
Gurdon/Elsevier# e0 r  f/ {  P$ O& `
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Dolly, Polly, and friends proved that somatic cells are potentially totipotent, but the reprogramming that a somatic cell nucleus must undergo during cloning remains an error-prone black box. James Byrne, John Gurdon, and colleagues (University of Cambridge, UK) have now shown that the biochemically tractable frog oocyte system can be used to model reprogramming. A modified version of their protocol might allow the isolation of elusive reprogramming factors and, eventually, the reprogramming of somatic human cells for self-transplantation of stem cells.) x" r# _* k$ y* l4 q
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The Cambridge group chose frog oocytes because, unlike most eggs, oocytes are not at all active in replication but very strongly so in transcription. To see if this transcriptional activity extended to reprogramming, Gurdon microinjected the oocytes with various cells: first mouse fetal fibroblasts, then mouse adult thymic cells, and finally human lymphocytes. All cell types eventually showed robust expression of oct4, whose expression is specific to and preserves the fate of stem cells.
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5 H! P; D9 D3 i( b7 rTranscriptional activity not associated with stem cells, such as that of ?-actin and the thymus marker thy-1, was reduced or extinguished by the transfer. But the extent of the transformation is not yet clear. "It's possible that what we are doing is turning everything into an oocyte," says Gurdon. But he believes that oct4 expression is a good sign that the cells are at least headed toward becoming stem cells.1 y- }' p2 \- v: a6 G# Y

+ E0 |2 a4 X# D' J4 F/ P9 zStem cell characteristics may develop only through sequential inductive events. But the optimists are hoping that there is a single extract that will do the entire conversion. The success of the current experiments, says Gurdon, is "one of the more compelling reasons for believing that to be true."" f1 a5 [2 {0 U

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  v  f& U% _& l  _& DByrne, J.A., et al. 2003. Curr. Biol. 13:1206–1213.(A frog oocyte germinal vesicle (center) )

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发表于 2025-5-31 10:58 |只看该作者
加油啊!!!!顶哦!!!!!  

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发表于 2025-5-27 13:51 |只看该作者
一楼的位置好啊..  

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发表于 2025-5-21 22:34 |只看该作者
干细胞之家微信公众号
看贴回复是好习惯  

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发表于 2025-5-18 18:41 |只看该作者
说的真有道理啊!

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发表于 2025-5-17 18:53 |只看该作者
帮你顶,人还是厚道点好  

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发表于 2025-5-16 20:33 |只看该作者
貌似我真的很笨????哎  

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发表于 2025-5-15 16:54 |只看该作者
好人一个  

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发表于 2025-5-14 13:21 |只看该作者
加油啊!偶一定会追随你左右,偶坚定此贴必然会起到抛砖引玉的作用~  

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发表于 2025-5-10 02:51 |只看该作者
…没我说话的余地…飘走  
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