PDF电子书：Fungal Genomics. Methods and Protocols MMB vol.722 --2011

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8 O+ |" H/ ~; d# h* i1 pFungal Genomics, J5 e6 k# w' W& p* B
Methods and Protocols
# |# U( _5 E& t3 x! g8 sSeries: Methods in Molecular Biology, Vol. 7228 C2 C* [6 ~" u/ D
Xu, Jin-Rong; Bluhm, Burton H. (Eds.)% n. n" a; n& O3 d9 B1 c
2011, XI, 285p. 29 illus..
, Z& q$ F) Q' e2 OA product of Humana Press, g, n- ]: U: y2 G
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1 Genome Sequencing and Assembly . . . . . . . . . . . . . . . . . .         1" I* O. o+ \' z+ k& t) {8 x+ {
Manfred G. Grabherr, Evan Mauceli, and Li-Jun Ma' ?- M  K( \2 T
2 Targeted Cloning of Fungal Telomeres . . . . . . . . . . .         117 C, @1 q7 d! C
Mark L. Farman
6 |4 a/ @5 g8 [: A: B3 Identification and Annotation of Repetitive Sequences in Fungal Genomes .         33
$ o, A! `7 F2 N0 A% ZBraham Dhillon and Stephen B. Goodwin
# X9 k- L0 Q) |& }& [  I4 Next-Generation Sequencing and Potential Applications in Fungal Genomics . .         51
. A4 d& |6 X- {# S- v- g+ xPhillip SanMiguel8 c- t$ p" _" i4 K! P% D
5 Getting the Most Out of Your Fungal Microarray Data:' V5 `! m+ I% Y$ K* j
Two Cost- and Time-Effective Methods . . . . . . . . . . . . . .         61
0 n4 W: o) G  \$ y2 k+ JSandra M. Mathioni, André Beló, Jeffrey P. Townsend,
. l& M) T0 w: u! Kand Nicole M. Donofrio3 |  W  S9 t. D6 V+ L' p2 S2 l
6 Fusarium graminearum from Expression Analysis to Functional Assays . . . . .         798 n3 H* [, b. q# ?$ E* D
Heather E. Hallen-Adams, Brad L. Cavinder, and Frances Trail
  o7 Q4 S; n+ u& M# g! o7 EST Analysis Pipeline: Use of Distributed Computing Resources . . . . . . . . .         1031 a& O3 r3 o9 c  V" h
Francisco Javier González and Juan Antonio Vizcaíno
# v+ N5 {; S7 K8 c6 t8 The Application of ChIP-chip Analysis in the Rice Blast Pathogen . . . . . .         121' [' Q% ^" t' r8 H- h1 Y1 s
Soonok Kim and Thomas K. Mitchell
* Z& U8 p- O* t1 ~2 U9 Proteome Studies of Filamentous Fungi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .         133
3 I* k# Q: f9 y' m4 ^1 Y" |4 mScott E. Baker and Ellen A. Panisko
; w0 W5 d& ~5 i8 d10 A Bioinformatics Pipeline for Sequence-Based Analyses2 }+ m2 `8 G# H* V5 u
of Fungal Biodiversity . . . . . . . . . . . . . . .         141
1 i$ O) x6 g4 @" H- nD. Lee Taylor and Shawn Houston
' y" Y. I1 Z9 {2 ]! X* [; U11 Identifying Protein Complexes by Affinity Purification& l# u# c! Z  E% S
and Mass Spectrometry Analysis in the Rice Blast Fungus . . . . . . . . . . . . . . . . . .         157
, `! k) i9 M4 h( x( }0 JWende Liu, Anton Iliuk, Andy Tao, and Shengli Ding  R! b; Z! J0 P0 }3 [* r
12 Large Scale Identification of Genes Involved in Plant–Fungal- s$ f% L% B: C" }' T/ ]" Z3 l& Y
Interactions Using Illumina’s Sequencing-by-Synthesis Technology . . . . . . . . . .         167  d  z( \0 S4 {& s8 p! H
R.C. Venu, Yuan Zhang, Brian Weaver, Peter Carswell,1 Y% S$ L3 E3 G/ O+ D# s
Thomas K. Mitchell, Blake C. Meyers, Michael J. Boehm,/ F1 I2 F, ]+ u: o2 E5 }! L
and Guo-Liang Wang
4 y' i) t5 ^3 |8 B4 R; T13 High-Throughput Production of Gene Replacement Mutants
1 q4 U+ _0 l+ oin Neurospora crassa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .         1792 v3 ?" r& x4 f3 M) U3 l) |; B! J
Gyungsoon Park, Hildur V. Colot, Patrick D. Collopy,4 c: N7 N2 w9 D: H5 ]# z, Q9 R( Y$ O
Svetlana Krystofova, Christopher Crew, Carol Ringelberg,
; o/ g) c3 K/ ?) ?3 Q  RLiubov Litvinkova, Lorena Altamirano, Liande Li, Susan Curilla,- q. V' q4 X3 j% T- Y9 \  j
Wei Wang, Norma Gorrochotegui-Escalante, Jay C. Dunlap,' |- q3 O2 R! M  u
and Katherine A. Borkovich1 @2 ~8 Z0 Z; o4 T& v5 n
14 Phenotypic Analysis of Neurospora crassa Gene Deletion Strains . . . . . . . . . . . . . .         191
2 k4 h6 K, i5 a2 v( ?, x, sGloria E. Turner8 d$ v1 L) Z: w+ V& |
15 Efficient Approaches for Generating GFP Fusion and Epitope-Tagging* E+ C' N/ A8 r: D' {2 H
Constructs in Filamentous Fungi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .         199  u( d, o6 q( u( C7 u9 l; A
Xiaoying Zhou, Guotian Li, and Jin-Rong Xu/ y. r. k& `% c& V- Y, b
16 Large-Scale Insertional Mutagenesis in Magnaporthe oryzae+ ^' u% o' V, ^* Z' H
by Agrobacterium tumefaciens-Mediated Transformation . . . . . . . . . . . . . . . . . . .         213: ^3 {0 o# c( e; O7 r
Xiao-Lin Chen, Jun Yang, and You-Liang Peng9 b4 H* N: q+ q. u7 y
17 Molecular Methods for Studying the Cryphonectria parasitica – Hypovirus6 u8 Y: O( j( g& I! i1 t! {! y4 f
Experimental System . . . . . . . . . . . . . . .         225
+ u8 ~' G. _0 G& a  N: O" ^Angus L. Dawe, Rong Mu, Gloricelys Rivera, and Joanna A. Salamon: k( K- k3 T$ [, p# E; X; Y/ |
18 Metabolic Fingerprinting in Fusarium verticillioides6 v' S) m+ ?8 i
to Determine Gene Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .         2374 Y" b0 ?/ H4 q9 d) ]9 o
Jonathon E. Smith and Burton H. Bluhm
# Z# N! f0 q' h" v. F19 Tapping Genomics to Unravel Ectomycorrhizal Symbiosis . . . . . . . . . . . . . . . . . .         2496 |3 d! [, X1 ^/ [* a. r
Jonathan M. Plett, Barbara Montanini, Annegret Kohler,
! [) X" R& M2 I! S3 WSimone Ottonello, and Francis Martin+ M' D9 K1 y- G$ p4 M/ \
Index . 2831 [# ^  d6 p  e! w
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