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本帖最后由 细胞海洋 于 2013-5-7 09:33 编辑 8 p& {$ l" o' s' D
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Spectroscopic Methods of Analysis
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- k8 A3 I2 M* U$ H9 w' M- VContents
q# f2 v$ Y2 a! JPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
7 f, n& E4 T8 o" I/ FContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix5 \. W3 ^% S) B+ Q0 ^: h/ N/ V
1 Fluorescence Lifetime Imaging Comes of Age How to Do It and0 C& J6 B& H7 ^/ v
How to Interpret It. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 U8 e, Y6 @5 H4 E$ a, A
Yi-Chun Chen, Bryan Q. Spring, and Robert M. Clegg
4 i7 `: z9 I! I: I2 Fo¨rster Resonance Energy Transfer and Trapping in Selected Systems:
# P$ Z( g+ j, H( ~5 W# bAnalysis by Monte-Carlo Simulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 230 Y* K! L3 S7 S; L9 A
P. Bojarski, A. Synak, L. Kułak, S. Rangelowa-Jankowska,6 c, {' @4 V5 m3 s
A. Kubicki, and B. Grobelna
1 S, ~0 W/ Y) ]5 I3 Molecular Organization of Polyene Antibiotic Amphotericin B Studied
, ^& `$ C: [' h+ {, D" Nby Means of Fluorescence Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 572 A1 I. s) i4 A6 Q7 X
Wieslaw I. Gruszecki, Rafal Luchowski, Piotr Wasko,! d) H: k4 q* S6 m
Zygmunt Gryczynski, and Ignacy Gryczynski
, ^9 d( z$ X% Z& y2 `4 Spectroscopic Probes of RNA Structure and Dynamics . . . . . . . . . . . . . . . . . . . . . . . 670 Z: ^$ g) C- t, G: J& U
Kathleen B. Hall* G7 R/ G# |5 B2 V" x! O
5 Fluorescence Methods to Study DNA Translocation and Unwinding
0 p: ^/ N) m( a1 w: i* `* y t0 ]Kinetics by Nucleic Acid Motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
+ \ ^6 o7 e- d9 J9 w, H1 gChristopher J. Fischer, Eric J. Tomko, Colin G. Wu, and Timothy M. Lohman7 b4 G3 W4 l0 e: e
6 Fluorescence Intensity, Anisotropy, and Transient Dynamic$ F7 o7 `# N- h' m# h$ u8 }2 O
Quenching Stopped-Flow Kinetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
6 |( \/ X, H' ?, s7 }Wlodek M. Bujalowski and Maria J. Jezewska
$ F7 [) J: A( E u, J+ s) |7 Using Structure–Function Constraints in FRET Studies
! j/ {# U& Z$ c4 Tof Large Macromolecular Complexes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135+ Z1 t$ r' E x& ]) Q
Wlodek M. Bujalowski and Maria J. Jezewska# P6 V" g5 @ a, I. F2 H* Y
8 Illuminating Allostery in Metal Sensing Transcriptional Regulators . . . . . . . . . . . . . 165
9 P( x. ~( |/ a7 XNicholas E. Grossoehme and David P. Giedroc$ W. W7 j9 r1 V9 p5 N
9 Fluorescence-Based Biosensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 193
# S5 M% p0 I6 p* F% Q }' }) nMaria Strianese, Maria Staiano, Giuseppe Ruggiero, Tullio Labella,
% G6 `% J) Q# U3 v8 B7 fClaudio Pellecchia, and Sabato D’Auria
" b+ {' o) g# I7 B4 A10 Metal-Enhanced Immunoassays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 217* y/ w# A6 q& X, ]+ K) K
Ignacy Gryczynski, Rafal Luchowski, Evgenia G. Matveeva, Tanya Shtoyko,5 N7 M: ^4 F: _: q5 w
Pabak Sarkar, Julian Borejdo, Irina Akopova, and Zygmunt Gryczynski3 O$ f3 ~' z2 a5 f; z
11 Initial Stages of Angiosperm Greening Monitored by Low-Temperature! q! j; Z8 h n9 `/ r9 M
Fluorescence Spectra and Fluorescence Lifetimes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 231
" [4 n: i8 u! B! n7 i, g. ^Beata Mysliwa-Kurdziel, Anna Stecka, and Kazimierz Strzalka; ~' j4 y, ^, u7 F
12 Activation of the Mammalian Cells by Using Light-Sensitive Ion Channels . . . . . . 241
7 u- {; b8 N) ~" |Mandy Siu Yu Lung, Paul Pilowsky, and Ewa M. Goldys, _6 N0 l+ B" U& y) u
13 Detection of Specific Strains of Viable Bacterial Pathogens by Using RNA8 R2 [& x: |: {- e! r8 h7 c
Bead Assays and Flow Cytometry with 2100 Bioanalyzer . . . . . . . . . . . . . . . . . . . . . 2531 _7 S2 q( Z* W& Y1 M3 }- K
Philip Butterworth, Henrique T.M.C.M. Baltar, Martin Kratzmeier,7 T! ?5 j2 `" f! n) p& g9 }6 O
and Ewa M. Goldys
2 W- @% m, C. j14 Photosynthetic Antenna Complex LHCII Studied with Novel
" U: w. d8 P, l! ^0 ^! _6 @ OFluorescence Techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 263& c& e+ a. s& [% Q
Wieslaw I. Gruszecki, Rafal Luchowski, Wojciech Grudzinski,
, S# u# Y6 C9 E( e( cZygmunt Gryczynski, and Ignacy Gryczynski
3 A* U9 \6 Q3 Y; o15 Analysis of RNA Folding and Ribonucleoprotein Assembly by Single-Molecule
2 X" f; [2 d, uFluorescence Spectroscopy. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2713 @& r/ j' g5 X
Goran Pljevaljcˇic′, Rae Robertson-Anderson, Edwin van der Schans,( `. o5 `4 f& g V/ [6 j# n
and David Millar1 h) M. `+ m0 \5 l
16 Single-Molecule Force Spectroscopy of Polycystic Kidney Disease Proteins . . . . . . 297
8 O7 Z5 R" U8 n3 kLiang Ma, Meixiang Xu, and Andres F. Oberhauser
s% ~* K; r+ d8 P; b9 M17 Single Molecule Detection Approach to Muscle Study: Kinetics9 N; l% N( O* y# H0 j
of a Single Cross-Bridge During Contraction of Muscle . . . . . . . . . . . . . . . . . . . . . . 3115 a: I" v5 l( g# R) ?5 G, Y4 s) z* S
Julian Borejdo, Danuta Szczesna-Cordary, Priya Muthu, Prasad Metticolla,! w1 r: W9 O" \- B# P: p
Rafal Luchowski, Zygmunt Gryczynski, and Ignacy Gryczynski
; r. l* _) m5 c7 b; p) `8 K8 c2 f5 d18 Single-Molecule Optical-Trapping Measurements with DNA Anchored
7 w9 j* ?" w! J$ s5 S- e, Jto an Array of Gold Nanoposts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 335
$ `1 }8 B( p7 S! u- a+ a; Y' HD. Hern Paik and Thomas T. Perkins0 B& C+ X% c6 b" L( \4 x
19 Electro-optical Analysis of Macromolecular Structure and Dynamics. . . . . . . . . . . . 357' Z) M3 U- p9 e3 Z( i
Dietmar Porschke" q" Z1 C+ n9 v) W# l6 Z* }
20 Cryoradiolysis and Cryospectroscopy for Studies of Heme-Oxygen7 T& S* P8 f0 l
Intermediates in Cytochromes P450 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375
/ q8 e" }: R ?; dI.G. Denisov, Y.V. Grinkova, and S.G. Sligar8 t5 E1 V9 B& b1 @* I
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6 b# t! x) Q5 [! s& G3 Q& v
. . . . . . . . . . . . . . . . . . . . . . . 393
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