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本帖最后由 细胞海洋 于 2013-5-7 09:33 编辑 ) I8 @& F. ^4 U" h+ C% `6 t
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Spectroscopic Methods of Analysis
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Contents& J, N$ w8 R) Q, ? Q
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
; J# }9 |8 x' E$ p* uContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix+ U. b( d& y; r8 ~
1 Fluorescence Lifetime Imaging Comes of Age How to Do It and
' R* W4 C1 }2 L# a" r% A6 `How to Interpret It. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
* @$ e6 m8 K2 A4 j* c7 Z8 K" ^' R) zYi-Chun Chen, Bryan Q. Spring, and Robert M. Clegg
9 l( M# |2 E2 D. Q2 Fo¨rster Resonance Energy Transfer and Trapping in Selected Systems:
: {, t+ i- a9 X# | u6 H6 G3 E* OAnalysis by Monte-Carlo Simulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
3 [# k/ x1 {) t4 ^. s# \P. Bojarski, A. Synak, L. Kułak, S. Rangelowa-Jankowska,
& r4 Y" N% I5 F5 j) \0 gA. Kubicki, and B. Grobelna' t- j; E( X: p5 D9 x0 o
3 Molecular Organization of Polyene Antibiotic Amphotericin B Studied$ Z8 Z! Q3 b( l$ b* N
by Means of Fluorescence Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
T* z' h! {/ s2 E7 S( x" cWieslaw I. Gruszecki, Rafal Luchowski, Piotr Wasko,# z" X% r# b0 K
Zygmunt Gryczynski, and Ignacy Gryczynski" k5 x* d+ Q1 X6 w+ S* v/ P
4 Spectroscopic Probes of RNA Structure and Dynamics . . . . . . . . . . . . . . . . . . . . . . . 67
. i @% N: x8 y! [9 IKathleen B. Hall
6 F, Z' ?* A! A* c9 _/ E6 ^0 Q1 t) i5 d5 Fluorescence Methods to Study DNA Translocation and Unwinding
3 O2 q3 j5 C5 M" qKinetics by Nucleic Acid Motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 851 x2 A$ j1 P0 r" N7 _
Christopher J. Fischer, Eric J. Tomko, Colin G. Wu, and Timothy M. Lohman
1 ]$ `( I6 n$ P" W6 Fluorescence Intensity, Anisotropy, and Transient Dynamic" u; o* X2 A' Z: m4 c( h
Quenching Stopped-Flow Kinetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
7 {/ d( i5 g! s0 j2 Y0 ]4 H- |& wWlodek M. Bujalowski and Maria J. Jezewska
2 W" p" f" W: F; h5 t8 w `1 g1 ~2 g7 Using Structure–Function Constraints in FRET Studies9 K w* Y e i2 Y
of Large Macromolecular Complexes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
* }' G4 }! \0 KWlodek M. Bujalowski and Maria J. Jezewska7 a8 `; ^' b! X; ]
8 Illuminating Allostery in Metal Sensing Transcriptional Regulators . . . . . . . . . . . . . 165
& E R; Q& `" a( U- e; e! }Nicholas E. Grossoehme and David P. Giedroc
& v* d% N/ [% @! x9 Fluorescence-Based Biosensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 193
7 h5 ~; T8 V, r/ o) P# O; tMaria Strianese, Maria Staiano, Giuseppe Ruggiero, Tullio Labella,+ \, N6 K7 z0 v9 G1 N2 J
Claudio Pellecchia, and Sabato D’Auria/ o2 Y0 G5 [6 W6 \3 ?
10 Metal-Enhanced Immunoassays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2176 T" d9 _/ u" t9 o7 }& | Q/ X
Ignacy Gryczynski, Rafal Luchowski, Evgenia G. Matveeva, Tanya Shtoyko,
( D2 U8 J* `2 a# T) |+ e, l# S0 IPabak Sarkar, Julian Borejdo, Irina Akopova, and Zygmunt Gryczynski
* a7 C" {3 @* E C/ _11 Initial Stages of Angiosperm Greening Monitored by Low-Temperature6 O5 ?" ?% o$ p" l1 J
Fluorescence Spectra and Fluorescence Lifetimes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 231
, F# n; O) U1 F+ s- K% t0 DBeata Mysliwa-Kurdziel, Anna Stecka, and Kazimierz Strzalka6 T, K; Z* J2 i
12 Activation of the Mammalian Cells by Using Light-Sensitive Ion Channels . . . . . . 241
7 c* k u1 r3 _; h. f( oMandy Siu Yu Lung, Paul Pilowsky, and Ewa M. Goldys8 T6 w a! c" u: a R W
13 Detection of Specific Strains of Viable Bacterial Pathogens by Using RNA* I% U( i5 l$ Y: F
Bead Assays and Flow Cytometry with 2100 Bioanalyzer . . . . . . . . . . . . . . . . . . . . . 2532 m9 Q; Z: J3 c1 z5 q0 G1 ~
Philip Butterworth, Henrique T.M.C.M. Baltar, Martin Kratzmeier,9 t! r. X$ e# G% ^$ x, V+ y
and Ewa M. Goldys
0 R# H* n; P4 c14 Photosynthetic Antenna Complex LHCII Studied with Novel( l. E f; R6 y1 O
Fluorescence Techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 263! F& b, B3 n. Z+ d( i: Y! I% g4 \
Wieslaw I. Gruszecki, Rafal Luchowski, Wojciech Grudzinski,
0 Z) h) X% G `; X+ mZygmunt Gryczynski, and Ignacy Gryczynski
" J) R+ v# x( P15 Analysis of RNA Folding and Ribonucleoprotein Assembly by Single-Molecule0 `( X. O2 |; G: z
Fluorescence Spectroscopy. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271/ Y/ m& c3 q5 U: c1 y! f
Goran Pljevaljcˇic′, Rae Robertson-Anderson, Edwin van der Schans,4 S0 X) G& Z# |0 F) L# X; D; b4 Z" v
and David Millar
) {/ R7 w7 y" k0 _5 z' D: {" y16 Single-Molecule Force Spectroscopy of Polycystic Kidney Disease Proteins . . . . . . 297 a$ \$ [( g# b# X. q
Liang Ma, Meixiang Xu, and Andres F. Oberhauser2 m4 O# Q! ~, v$ C+ l' B% Q. [
17 Single Molecule Detection Approach to Muscle Study: Kinetics1 i7 I* F% ]! r$ k9 [
of a Single Cross-Bridge During Contraction of Muscle . . . . . . . . . . . . . . . . . . . . . . 3115 z' d9 g0 k0 u4 `8 y6 f0 U& y9 A
Julian Borejdo, Danuta Szczesna-Cordary, Priya Muthu, Prasad Metticolla,
( w1 ^4 V+ w! ?; x# t/ SRafal Luchowski, Zygmunt Gryczynski, and Ignacy Gryczynski$ h4 f- ?' n8 `( b; X7 O) `; q
18 Single-Molecule Optical-Trapping Measurements with DNA Anchored) n* Y% r& `8 |7 t- s1 K5 [. X
to an Array of Gold Nanoposts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 335
* N6 X' c" X$ D# R. }1 f; ^D. Hern Paik and Thomas T. Perkins
% _6 |& i& z; U! U19 Electro-optical Analysis of Macromolecular Structure and Dynamics. . . . . . . . . . . . 357
1 @) m2 |$ I9 `% e0 zDietmar Porschke. z5 D5 q, u) `' Q6 g) T4 u' a
20 Cryoradiolysis and Cryospectroscopy for Studies of Heme-Oxygen( C7 p) r( N/ _+ [$ b
Intermediates in Cytochromes P450 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375
# D* U1 c D/ DI.G. Denisov, Y.V. Grinkova, and S.G. Sligar
, l! _' c ~8 h/ q9 JIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ." H7 g `7 K) I2 q% m6 f* [" Z9 X S
. . . . . . . . . . . . . . . . . . . . . . . 3931 _, q! Z; D% Q9 G9 h, I- j
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