PDF电子书：Spectroscopic Methods of Analysis

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- J5 i# e# h$ FSpectroscopic Methods of Analysis
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Contents
6 y; c; W5 L# p9 H5 ^/ Q- bPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
' H8 u$ G1 w$ C* J( Y5 XContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix- W6 D0 W( k4 c) Z/ C6 ?9 g" _
1 Fluorescence Lifetime Imaging Comes of Age How to Do It and" ~4 M* S# A7 ?
How to Interpret It. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 P3 N/ P2 _' X( L) R/ L
Yi-Chun Chen, Bryan Q. Spring, and Robert M. Clegg$ I& [/ [* a3 _3 l& e' w1 p# S( S4 n
2 Fo¨rster Resonance Energy Transfer and Trapping in Selected Systems:
, }7 @+ U, q2 ~4 GAnalysis by Monte-Carlo Simulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
) M/ j& d6 M/ v( vP. Bojarski, A. Synak, L. Kułak, S. Rangelowa-Jankowska,% `& P; `  y; m% z/ N
A. Kubicki, and B. Grobelna" z! K, c3 i) Q4 E; J% s+ a! ^
3 Molecular Organization of Polyene Antibiotic Amphotericin B Studied
# d1 X/ p0 a9 j! w) D1 [by Means of Fluorescence Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57& [; P+ I; H% d$ `
Wieslaw I. Gruszecki, Rafal Luchowski, Piotr Wasko,
1 s: Q5 k- ]* {0 N6 H% s; P9 kZygmunt Gryczynski, and Ignacy Gryczynski
! a- o7 s; n# @% y4 Spectroscopic Probes of RNA Structure and Dynamics . . . . . . . . . . . . . . . . . . . . . . . 671 ]4 o! i& Y8 K1 I! g- l4 b0 z
Kathleen B. Hall
6 N% v4 ~* r! o' \. P7 V6 i5 Fluorescence Methods to Study DNA Translocation and Unwinding
$ @# e* D( C0 {* ?4 J$ ^9 D. aKinetics by Nucleic Acid Motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
  l3 c2 I  W6 bChristopher J. Fischer, Eric J. Tomko, Colin G. Wu, and Timothy M. Lohman) b; i; x/ Z/ P  m5 Q8 D. x" i; a
6 Fluorescence Intensity, Anisotropy, and Transient Dynamic
% p# ~, p* w! ]3 N0 ?0 H8 y/ {$ bQuenching Stopped-Flow Kinetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105( u6 {- q  c* n4 z4 y9 X* r
Wlodek M. Bujalowski and Maria J. Jezewska
( w" [' v6 t9 {( n$ t& N7 Using Structure–Function Constraints in FRET Studies$ L) ~, k5 q. d, ]$ K; j  E6 i
of Large Macromolecular Complexes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
" q0 S7 X# X- H0 v  m$ AWlodek M. Bujalowski and Maria J. Jezewska+ O2 q& \8 l6 r( _: a. R# `  h
8 Illuminating Allostery in Metal Sensing Transcriptional Regulators . . . . . . . . . . . . . 165
% {# S( E3 q+ ?" T- p) CNicholas E. Grossoehme and David P. Giedroc2 D- F3 T6 D0 P; M
9 Fluorescence-Based Biosensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 193
, H: `9 b# o4 {7 F! H  ~! Q8 C! HMaria Strianese, Maria Staiano, Giuseppe Ruggiero, Tullio Labella,
1 a# J5 \# N! l+ @3 Z) jClaudio Pellecchia, and Sabato D’Auria8 U% l1 |) {6 }% Y
10 Metal-Enhanced Immunoassays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2176 k0 T( |/ [! p& R- }( _" y3 ]: q- C
Ignacy Gryczynski, Rafal Luchowski, Evgenia G. Matveeva, Tanya Shtoyko,) P1 @! }! o- G( u; y% d7 s$ ^# H
Pabak Sarkar, Julian Borejdo, Irina Akopova, and Zygmunt Gryczynski
' h. \9 B4 p" j7 w2 Z11 Initial Stages of Angiosperm Greening Monitored by Low-Temperature
6 A# n6 k) Q/ g8 G1 M5 xFluorescence Spectra and Fluorescence Lifetimes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 231
2 q( V+ I; m3 `* q& P1 x5 |Beata Mysliwa-Kurdziel, Anna Stecka, and Kazimierz Strzalka7 p7 W: ?3 ~; t9 I
12 Activation of the Mammalian Cells by Using Light-Sensitive Ion Channels . . . . . . 241
  k: o, L( t. g( v- jMandy Siu Yu Lung, Paul Pilowsky, and Ewa M. Goldys7 h: G3 ^6 A: O
13 Detection of Specific Strains of Viable Bacterial Pathogens by Using RNA
5 _# Q" L. l0 C2 ?% F! V! [" u1 uBead Assays and Flow Cytometry with 2100 Bioanalyzer . . . . . . . . . . . . . . . . . . . . . 253
# e7 {5 R2 e( |- EPhilip Butterworth, Henrique T.M.C.M. Baltar, Martin Kratzmeier," ^2 v, J4 Q9 N$ u; x2 @7 Y
and Ewa M. Goldys
3 H! W$ M8 q# y6 V14 Photosynthetic Antenna Complex LHCII Studied with Novel4 B2 [0 W5 ]! Z) m
Fluorescence Techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 263" ^) u/ g+ X) l$ C( h
Wieslaw I. Gruszecki, Rafal Luchowski, Wojciech Grudzinski,* t' b5 o/ x8 {& o, |$ C
Zygmunt Gryczynski, and Ignacy Gryczynski- S" Z. M& m4 D+ x' Q0 F8 d+ X, {
15 Analysis of RNA Folding and Ribonucleoprotein Assembly by Single-Molecule
# h$ O. s2 J- c+ ~0 u, p( HFluorescence Spectroscopy. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271! I4 h, R0 W% g$ \
Goran Pljevaljcˇic′, Rae Robertson-Anderson, Edwin van der Schans,
8 ~% Y  {! T; [9 s9 Q6 Nand David Millar/ n- z8 Y# N+ w- w: {3 b2 H5 u
16 Single-Molecule Force Spectroscopy of Polycystic Kidney Disease Proteins . . . . . . 297
$ c1 t9 O! [& Y; pLiang Ma, Meixiang Xu, and Andres F. Oberhauser/ ]% f: h% {% q% g/ S9 k- W
17 Single Molecule Detection Approach to Muscle Study: Kinetics% P% |/ O6 g7 E4 i4 d
of a Single Cross-Bridge During Contraction of Muscle . . . . . . . . . . . . . . . . . . . . . . 311
$ C1 w$ ^/ f/ C' V1 N2 MJulian Borejdo, Danuta Szczesna-Cordary, Priya Muthu, Prasad Metticolla,/ }; u: H  \, D  C) D+ N* o8 B
Rafal Luchowski, Zygmunt Gryczynski, and Ignacy Gryczynski
5 r/ C7 M5 R' v( [- Z18 Single-Molecule Optical-Trapping Measurements with DNA Anchored
: L7 f; Q* q0 E6 o! D4 r7 H! ?4 Bto an Array of Gold Nanoposts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 335
7 I) z1 l. K! G6 A4 s1 E' TD. Hern Paik and Thomas T. Perkins# ]  F( Y; C4 N- Q( G- B
19 Electro-optical Analysis of Macromolecular Structure and Dynamics. . . . . . . . . . . . 357
# T; O" B9 t+ YDietmar Porschke+ F+ ^# q: F" G, O0 K
20 Cryoradiolysis and Cryospectroscopy for Studies of Heme-Oxygen: E5 D$ D* }! B' S
Intermediates in Cytochromes P450 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375; i) K" D, D6 i5 h- u
I.G. Denisov, Y.V. Grinkova, and S.G. Sligar+ o% H6 C! B- h% b+ a& e
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .0 b; V8 W: t1 a- A
. . . . . . . . . . . . . . . . . . . . . . . 393
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