|
- 积分
- 56
- 威望
- 56
- 包包
- 422
|
本帖最后由 细胞海洋 于 2013-5-7 09:33 编辑
6 Z1 f" C0 Y/ Z
( o7 ?3 x! B2 D+ J6 f5 WRecombinant and In Vitro RNA Synthesis0 W$ u7 J' C0 O1 b# I9 U5 g
Contents
: d3 Z. b- K6 V; @Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v& D k/ U% O T" F) M& a) C
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
, [8 o* O7 J" u1 o* }/ w1 Purification of RNA Expressed In Vivo Inserted in a tRNA Scaffold . . . . . . . . 1" q6 A# c* r( u. F* `& ]
Luc Ponchon and Frédéric Dardel4 { A* ^) A% j; w7 }4 N U
2 Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold. . . . . . . . . 9
5 L; z& _) m6 g- \) n* i& e3 PLuc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte,
# T) v& g% C6 A# B4 }0 Xand Frédéric Dardel3 i$ O. `9 X. i7 S/ g
3 Preparation of Long Templates for RNA In Vitro Transcription7 ^- ~2 T9 h& S% t Y
by Recursive PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 194 [4 `$ `6 @2 K
Jessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy,
# o* e7 B% {; t' ?: |0 V: _+ dand Loren Dean Williams
8 j# y5 E' u: X+ I! e4 General Protocols for Preparation of Plasmid DNA Template,
. {; u( U6 B1 M3 i# [( URNA In Vitro Transcription, and RNA Purification by Denaturing PAGE . . . . 435 U0 W2 q' I4 K, ^' w
Jo L. Linpinsel and Graeme L. Conn
7 n9 Z" Z' R) @) @8 |- q9 t5 Preparation of Short RNA by In Vitro Transcription . . . . . . . . . . . . . . . . . . . . 59
[6 ^8 r9 K/ wCheng Lu and Pingwei Li
! h+ p* O, u* e* y; k" d6 Native RNA Purification by Gel Filtration Chromatography . . . . . . . . . . . . . . 69& Q' f( K+ O% b1 i( W
Evan P. Booy, Hui Meng, and Sean A. McKenna
# I( w. X; n7 Q- l' y" c& U7 Cis-Acting Ribozymes for the Production of RNA In Vitro N0 f {& E# u0 c' {4 [/ W
Transcripts with Defined 5′ and 3′ Ends . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
( ?9 r. Q7 W; N; ]) u! TJohanna M. Avis, Graeme L. Conn, and Scott C. Walker
5 K# a( d0 } F8 Trans-Acting Antigenomic HDV Ribozyme for Production
* F0 p# p. L" K9 X# H" J: V; U8 Pof In Vitro Transcripts with Homogenous 3′ Ends . . . . . . . . . . . . . . . . . . . . . 996 ~/ I# _# ^" Y0 \) `6 @) _9 u
Milena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski,# k% \% {2 W( S* r. q9 D1 l$ F9 `6 S
and Jerzy Ciesiolka
$ _8 ^. U' x$ |4 f9 Z9 Rapid Preparation of RNA Samples Using DNA-Affinity Chromatography: w4 [ }' o Z
and DNAzyme Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1131 V/ S6 I5 I8 K* \
Hae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong" i( w3 U; T7 U6 w# i
10 Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA . . 1239 e- J0 K) F- @
Geneviève Di Tomasso, Philipe Lampron, James G. Omichinski,
$ s4 l' C3 i* U% _# g: Z( {and Pascale Legault
7 R# V" [7 e5 L! S: [1 T) `11 Affinity Purification of RNA Using an ARiBo Tag . . . . . . . . . . . . . . . . . . . . . . 1370 L9 \& a; R3 K
Geneviève Di Tomasso, Pierre Dagenais, Alexandre Desjardins,8 e& |/ e0 s# P$ X1 U8 A2 y
Alexis Rompré-Brodeur, Vanessa Delfosse, and Pascale Legault
' [! p" T# y% e( |) A12 Plasmid Template Design and In Vitro Transcription of Short RNAs$ R( R7 R' n8 O' O9 s. W9 I
Within a “Structure Cassette” for Structure Probing Experiments . . . . . . . . . . 1576 v _" |8 F6 a* U8 q8 M" R! M( \# x4 L
13 In Vitro Transcription of Modified RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171
: a- W& ~$ T: z' {6 lStephanie L. Moon and Jeffrey Wilusz+ `5 I& D, I$ o, Z
14 End-Labeling Oligonucleotides with Chemical Tags After Synthesis . . . . . . . . 181* x) y& ^! ?3 B" K1 T4 J: c
N. Ruth Zearfoss and Sean P. Ryder
: z' I; v& Y4 q15 High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA. . . . . . . . 195 p0 Y9 P6 p9 I' X7 } x
Ya-Ming Hou
" }1 ^. K: u+ Y5 A2 K1 Z16 Se-Derivatized RNAs for X-ray Crystallography. . . . . . . . . . . . . . . . . . . . . . . . 213( S: [/ X V4 y# b% ?. G9 W3 r
Lina Lin and Zhen Huang. _: P) q1 }% H+ ^6 _# p: t B
17 Biosynthetic Preparation of 13C/15N-Labeled rNTPs for High-Resolution
( ~: K" Y& N+ h( ]1 [& QNMR Studies of RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 227; E9 o* ~; c/ h' `9 B* R5 n' Z
Luigi Martino and Maria R. Conte
% x* X$ k2 D) z k ]9 @, J+ h18 Preparative Separation of Ribonucleoside Monophosphates
( K) Y7 C2 U# a; ^! }' rby Ion-Pair Reverse-Phase HPLC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247& |9 x+ p/ c, G6 K+ T2 O+ Y `
Pierre Dagenais and Pascale Legault6 y( L7 |; [6 A
19 Splint Ligation of RNA with T4 DNA Ligase . . . . . . . . . . . . . . . . . . . . . . . . . 257
, }' f, W+ H aChristopher J. Kershaw and Raymond T. O’Keefe
3 N: |9 g3 n9 a9 Y- RIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2711 g7 p$ p! v# F" U/ R1 Y
& ^# ~- Z+ D* C' S
[hide][/hide] |
附件: 你需要登录才可以下载或查看附件。没有帐号？注册
-
总评分: 包包 + 10
查看全部评分
|
发表于 2013-5-6 12:53
