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本帖最后由 细胞海洋 于 2013-5-7 09:33 编辑 ) ?1 D9 z1 d1 i! j: }0 P& L
: b0 ]8 u$ v) b- N$ nRecombinant and In Vitro RNA Synthesis
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Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v# h5 Z) ?! p2 X h
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii+ z, x0 Z& G$ \2 K0 C5 U
1 Purification of RNA Expressed In Vivo Inserted in a tRNA Scaffold . . . . . . . . 1
& z* b' l3 w+ @7 a/ B9 CLuc Ponchon and Frédéric Dardel; |4 V; r3 u2 s
2 Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold. . . . . . . . . 9
1 X* U0 ` |" G* e0 ^7 yLuc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte,. i4 O9 X5 B2 G
and Frédéric Dardel" ^/ K2 W& d$ N# B
3 Preparation of Long Templates for RNA In Vitro Transcription4 z( m A: v* P7 }* b* {! v) o B2 w
by Recursive PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
. t- s1 N, n2 N/ ^2 P8 z9 ~1 QJessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy,
7 f/ D: O/ b& pand Loren Dean Williams
( [1 j$ {. h) _. C4 General Protocols for Preparation of Plasmid DNA Template,( X8 |# M( s; P, i2 o+ Q+ X5 m9 K
RNA In Vitro Transcription, and RNA Purification by Denaturing PAGE . . . . 43
9 }8 S. |( D9 TJo L. Linpinsel and Graeme L. Conn* \' d* J! j1 ?
5 Preparation of Short RNA by In Vitro Transcription . . . . . . . . . . . . . . . . . . . . 592 }( N5 Z8 [* Y0 I, [; j# I2 A& D( q) d
Cheng Lu and Pingwei Li
9 i7 y( ^ u) N* U3 K6 Native RNA Purification by Gel Filtration Chromatography . . . . . . . . . . . . . . 694 X# W1 e/ v7 B% S8 }! ~
Evan P. Booy, Hui Meng, and Sean A. McKenna1 f* P! h0 f3 y, l. }. B9 L
7 Cis-Acting Ribozymes for the Production of RNA In Vitro
# _$ x. g1 j3 o. ~. x( X3 sTranscripts with Defined 5′ and 3′ Ends . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
9 l7 j9 I7 J+ ]Johanna M. Avis, Graeme L. Conn, and Scott C. Walker
2 ~' s* q3 M+ C; H _4 `8 Trans-Acting Antigenomic HDV Ribozyme for Production
4 }, b2 U; x! l! E- z0 @of In Vitro Transcripts with Homogenous 3′ Ends . . . . . . . . . . . . . . . . . . . . . 99
4 f# _+ I2 N! E- H6 eMilena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski,
0 F y3 {; C+ V6 Y. s1 F5 cand Jerzy Ciesiolka
2 \3 S3 H: h+ h/ s& v3 g4 V6 s9 i9 Rapid Preparation of RNA Samples Using DNA-Affinity Chromatography
" w9 w) }# _/ G6 g+ Xand DNAzyme Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
3 ?. s& B3 [3 E2 F I8 S2 bHae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong; }: R: a5 g8 E8 o3 L. g
10 Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA . . 123
' ?- |) D4 _2 w+ `% [& SGeneviève Di Tomasso, Philipe Lampron, James G. Omichinski,7 V4 T2 |; f6 Q
and Pascale Legault
: G+ o1 {$ c _. R+ q11 Affinity Purification of RNA Using an ARiBo Tag . . . . . . . . . . . . . . . . . . . . . . 137: o5 ?" U7 w0 X, L Q0 h2 S7 z$ C
Geneviève Di Tomasso, Pierre Dagenais, Alexandre Desjardins,
# K, E3 r) C4 v( k) KAlexis Rompré-Brodeur, Vanessa Delfosse, and Pascale Legault$ z( ~- Q) I; N6 V0 ?, w" v
12 Plasmid Template Design and In Vitro Transcription of Short RNAs
6 |1 N) Z1 \6 [* Q4 ~Within a “Structure Cassette” for Structure Probing Experiments . . . . . . . . . . 157
2 M& j- q& {/ O! L13 In Vitro Transcription of Modified RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1718 P2 G; I/ Q+ V9 _
Stephanie L. Moon and Jeffrey Wilusz
; d7 {7 J+ V4 y% w. q14 End-Labeling Oligonucleotides with Chemical Tags After Synthesis . . . . . . . . 181 y* q2 K3 y# @- E; j
N. Ruth Zearfoss and Sean P. Ryder
i, Q v% F" w# \& t15 High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA. . . . . . . . 1953 }& W0 c2 s b$ y! a
Ya-Ming Hou
9 Y( s/ t+ |7 \" S0 H16 Se-Derivatized RNAs for X-ray Crystallography. . . . . . . . . . . . . . . . . . . . . . . . 213
9 L0 l: i3 C- b1 d# P# D1 f2 yLina Lin and Zhen Huang
% r0 I( C2 P% O- q4 w/ C; o2 x17 Biosynthetic Preparation of 13C/15N-Labeled rNTPs for High-Resolution! F5 [% M5 A9 e$ s: y- u. K8 ]
NMR Studies of RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 227
" K! y2 R! P0 s# E% \0 \" OLuigi Martino and Maria R. Conte! k3 d H9 v9 P, a' k( X- Y
18 Preparative Separation of Ribonucleoside Monophosphates, c: A5 N$ z1 J; l2 b$ y
by Ion-Pair Reverse-Phase HPLC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247
& U5 @0 x* }% }- q, q7 P$ ^7 bPierre Dagenais and Pascale Legault
8 F% P1 e# c# h5 j19 Splint Ligation of RNA with T4 DNA Ligase . . . . . . . . . . . . . . . . . . . . . . . . . 257
0 {9 j& E, J) x2 {; U' r5 bChristopher J. Kershaw and Raymond T. O’Keefe' y9 y# R. l/ `7 z# m
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
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发表于 2013-5-6 12:53
