PDF电子书：Recombinant and In Vitro RNA Synthesis

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Recombinant and In Vitro RNA Synthesis; I+ ^; j0 ]7 N! V& p, m; {
Contents
3 `! F6 v5 ?) Z9 e+ w: i# E4 wPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v4 V6 H: E5 j% }2 f
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
, R, s  o$ C# m$ E$ E1 Purification of RNA Expressed In Vivo Inserted in a tRNA Scaffold . . . . . . . . 1
0 a: ]$ j7 w: C& Q* \Luc Ponchon and Frédéric Dardel
' |* E. ~1 U& U4 W- }, S2 Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold. . . . . . . . . 9
1 J% F4 G. l3 |- YLuc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte,, o$ ?8 P, M$ u& O" T
and Frédéric Dardel
) y. j7 {) d5 L. B3 Preparation of Long Templates for RNA In Vitro Transcription
9 j! a5 g/ }( A- N. F  H4 Zby Recursive PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 199 h: ]1 D2 c  l% l( }8 _* J" o
Jessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy,
8 ^% o: Z/ o2 Z  \and Loren Dean Williams
% [1 C1 a' r' U$ b. }4 General Protocols for Preparation of Plasmid DNA Template,) h/ i$ m& V9 f! C6 u
RNA In Vitro Transcription, and RNA Purification by Denaturing PAGE . . . . 430 y; v3 l5 Z; c
Jo L. Linpinsel and Graeme L. Conn
; }+ t: {# o8 \6 ^% A6 q$ d5 Preparation of Short RNA by In Vitro Transcription . . . . . . . . . . . . . . . . . . . . 59, e2 X8 T4 c4 @: F: _9 e' F
Cheng Lu and Pingwei Li
& u3 Z4 T& E* i5 n7 f6 Native RNA Purification by Gel Filtration Chromatography . . . . . . . . . . . . . . 69
: Y0 J5 Y5 F2 a# f2 Q1 OEvan P. Booy, Hui Meng, and Sean A. McKenna, f. U1 u) l7 R1 U) g; H
7 Cis-Acting Ribozymes for the Production of RNA In Vitro
, r$ e5 e! Y! g( |3 kTranscripts with Defined 5′ and 3′ Ends . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
2 W3 J# e5 I) {/ eJohanna M. Avis, Graeme L. Conn, and Scott C. Walker
. F; s& j* i, N8 Trans-Acting Antigenomic HDV Ribozyme for Production& j4 K3 y6 n+ @
of In Vitro Transcripts with Homogenous 3′ Ends . . . . . . . . . . . . . . . . . . . . . 994 ?2 C' i+ S6 n
Milena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski,( o% X9 F, r8 a  h: ~
and Jerzy Ciesiolka$ K+ s' z3 F0 |
9 Rapid Preparation of RNA Samples Using DNA-Affinity Chromatography
1 c" _7 }" W# l2 @4 ]! Uand DNAzyme Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
0 a4 m$ {$ ]. f" o5 m" \* oHae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong
4 x" V! F: Q: ]' z/ ]1 ~1 q; S2 ^- S10 Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA . . 1232 p& @! A1 `: H: m/ I! k% ^4 Y& z9 Q: O
Geneviève Di Tomasso, Philipe Lampron, James G. Omichinski,  }; ~! R* l) V0 I& a4 U: u% y% H" @. O, i
and Pascale Legault
+ T8 e( }- H+ O1 k6 D+ \11 Affinity Purification of RNA Using an ARiBo Tag . . . . . . . . . . . . . . . . . . . . . . 137
/ f- m" J# P2 F8 [* H2 T! \* }) Q6 SGeneviève Di Tomasso, Pierre Dagenais, Alexandre Desjardins,
1 X' M) d7 d! Q* i# N% e- aAlexis Rompré-Brodeur, Vanessa Delfosse, and Pascale Legault
8 r$ h- W! T; z! b12 Plasmid Template Design and In Vitro Transcription of Short RNAs  z: Y. _) i4 f' {
Within a “Structure Cassette” for Structure Probing Experiments . . . . . . . . . . 157
9 j$ M4 O/ E) f8 s13 In Vitro Transcription of Modified RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171
# N/ n3 ~0 ^# n' z1 rStephanie L. Moon and Jeffrey Wilusz, S7 n. W0 V" r0 m9 e% b! Y  u
14 End-Labeling Oligonucleotides with Chemical Tags After Synthesis . . . . . . . . 181
% Y5 x! @; E; j' y/ w/ B, ?+ xN. Ruth Zearfoss and Sean P. Ryder
: ?, ]0 ~" G, U3 R15 High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA. . . . . . . . 1956 l5 O3 D( U2 W. s! }/ ]/ p
Ya-Ming Hou% V* Z8 b5 S2 n2 s; `
16 Se-Derivatized RNAs for X-ray Crystallography. . . . . . . . . . . . . . . . . . . . . . . . 213; _5 c3 i/ t" t2 g( E$ e8 E4 u( E
Lina Lin and Zhen Huang7 c5 C# A4 ^0 g. C+ \/ [- e( g- q
17 Biosynthetic Preparation of 13C/15N-Labeled rNTPs for High-Resolution+ l% g. p) @9 L! J
NMR Studies of RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 227, ^/ I8 a; @8 A1 C! O
Luigi Martino and Maria R. Conte/ i, L) W/ U/ J2 F9 T: T" L6 I: _
18 Preparative Separation of Ribonucleoside Monophosphates
0 n* Q1 h5 i; B! n1 A/ uby Ion-Pair Reverse-Phase HPLC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247
, [: U( F# ~4 q7 i+ H: _# MPierre Dagenais and Pascale Legault
0 V4 e; {/ v# {" O% V. \19 Splint Ligation of RNA with T4 DNA Ligase . . . . . . . . . . . . . . . . . . . . . . . . . 257
) E& s- z) i& KChristopher J. Kershaw and Raymond T. O’Keefe; `2 o: O2 ~2 j$ v3 @
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
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