PDF电子书：Recombinant and In Vitro RNA Synthesis

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1 X$ V& U; z" ~& E4 |Recombinant and In Vitro RNA Synthesis8 G! H( N; [* e$ E" D, H$ `  d
Contents
; X8 P' T  k- \! L4 }1 rPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
- q! U0 n& [. UContributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
) Z' q% q8 f5 U" x9 ~1 Purification of RNA Expressed In Vivo Inserted in a tRNA Scaffold . . . . . . . . 1' a1 j8 b3 F( j# @: }2 I- |  F
Luc Ponchon and Frédéric Dardel. }6 f' k" @. ?  e! m
2 Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold. . . . . . . . . 95 b0 [1 o3 N0 q7 Z% m1 ^
Luc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte,( |& k& B! y/ s
and Frédéric Dardel1 h* h) ^6 S" J8 k1 S
3 Preparation of Long Templates for RNA In Vitro Transcription
$ [' w: M0 u' V1 Fby Recursive PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19- w- g( H5 c! f1 R0 }2 z
Jessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy,
$ L4 z# |9 G8 L6 X# K& o2 xand Loren Dean Williams
1 h8 Y9 p$ ?, m4 x% Y- ]) Q4 General Protocols for Preparation of Plasmid DNA Template,
! e# {/ i& b* K( X* I' DRNA In Vitro Transcription, and RNA Purification by Denaturing PAGE . . . . 43
  S5 |( {4 v  d$ w( [Jo L. Linpinsel and Graeme L. Conn" c0 }, _" X- u8 q1 Y  d  F
5 Preparation of Short RNA by In Vitro Transcription . . . . . . . . . . . . . . . . . . . . 59" g( T7 R; O. k3 j! z8 Q
Cheng Lu and Pingwei Li
" v: @* f! N! U1 K/ n7 u7 ]6 Native RNA Purification by Gel Filtration Chromatography . . . . . . . . . . . . . . 699 f& b* G; j& d4 Z" _
Evan P. Booy, Hui Meng, and Sean A. McKenna
& O9 b1 ?) _, _0 K7 Cis-Acting Ribozymes for the Production of RNA In Vitro
0 a$ E( S; t% ^- S% tTranscripts with Defined 5′ and 3′ Ends . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 831 M3 j4 ?5 v2 v, X' K. ?- G/ x0 Q
Johanna M. Avis, Graeme L. Conn, and Scott C. Walker. U. X7 q0 ^3 [6 c8 @% Q
8 Trans-Acting Antigenomic HDV Ribozyme for Production/ V5 u1 y3 |# ]$ O' J0 _
of In Vitro Transcripts with Homogenous 3′ Ends . . . . . . . . . . . . . . . . . . . . . 99
) l8 a# C: S( o! |; }Milena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski,
; V* t0 y: Y8 L5 u& D- N# o' ~and Jerzy Ciesiolka$ Y+ b, p: F6 y! _: d! g
9 Rapid Preparation of RNA Samples Using DNA-Affinity Chromatography. h( J' ^6 U" k4 J
and DNAzyme Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1135 t& B. {) O! d& P9 c
Hae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong# R+ p5 L; s/ P0 N4 d$ V7 e" A
10 Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA . . 123  T0 F+ F* P! c" v( L- ?# n
Geneviève Di Tomasso, Philipe Lampron, James G. Omichinski,
) L+ |3 Y2 _$ x: B) @8 e/ Y4 xand Pascale Legault; Z- Y% \0 `2 p4 U
11 Affinity Purification of RNA Using an ARiBo Tag . . . . . . . . . . . . . . . . . . . . . . 137
7 m* ~. }1 N  ]6 z8 HGeneviève Di Tomasso, Pierre Dagenais, Alexandre Desjardins,1 p6 B/ m! Q! ]! l* e3 P% p
Alexis Rompré-Brodeur, Vanessa Delfosse, and Pascale Legault6 [5 k9 [' ]3 {, z2 k; j7 C
12 Plasmid Template Design and In Vitro Transcription of Short RNAs- j6 V3 J8 `6 c3 K8 U
Within a “Structure Cassette” for Structure Probing Experiments . . . . . . . . . . 157
( H! r8 N; e) q2 A( X  A1 G13 In Vitro Transcription of Modified RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1717 u3 Q" d3 k8 C
Stephanie L. Moon and Jeffrey Wilusz
, Q# J4 s. `$ x* ?1 Y14 End-Labeling Oligonucleotides with Chemical Tags After Synthesis . . . . . . . . 181
' i" D( n/ a# u# r: |N. Ruth Zearfoss and Sean P. Ryder
3 E+ d4 o9 ]/ X! D. D9 I" h) V! r15 High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA. . . . . . . . 195( ]+ i$ s2 L% H! n3 K
Ya-Ming Hou4 _2 d4 N3 p4 s7 t
16 Se-Derivatized RNAs for X-ray Crystallography. . . . . . . . . . . . . . . . . . . . . . . . 213
( v7 c& n% t. r" R' [Lina Lin and Zhen Huang5 i0 @' `! ^7 ?( J
17 Biosynthetic Preparation of 13C/15N-Labeled rNTPs for High-Resolution9 m0 l1 l5 f: y6 l+ [9 [: Z
NMR Studies of RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 227, @+ @, x8 u1 n1 N
Luigi Martino and Maria R. Conte
4 j4 ^8 [4 i% v. ^18 Preparative Separation of Ribonucleoside Monophosphates# X/ A. }) O. {
by Ion-Pair Reverse-Phase HPLC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247, J  Q$ K7 U- W2 n
Pierre Dagenais and Pascale Legault* d% A+ |% @; \8 Z3 [. M
19 Splint Ligation of RNA with T4 DNA Ligase . . . . . . . . . . . . . . . . . . . . . . . . . 257  }% I0 [. Q  ^1 \- D
Christopher J. Kershaw and Raymond T. O’Keefe
- r$ L6 c2 r$ D3 ~' s0 K) gIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271( u1 A5 J# H: H# i; y

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