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[实验技术类] PDF电子书:Chromatin Remodeling [复制链接]

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发表于 2013-5-6 14:06 |只看该作者 |正序浏览 |打印
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Chromatin Remodeling8 I, h) P, z# I

% y$ U# B1 s+ X$ O8 LContents# S: Z4 t) V/ U& @
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v" s! k1 T( |, c/ C; q7 v5 Y9 n
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix- u6 C& K) \# n: ]' j: j; _" m
1 Strain Construction and Screening Methods for a Yeast Histone H3/H4
0 O  S) E; X  T, N; p: EMutant Library. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
5 }) N& X# Z4 y6 Y% s( KJunbiao Dai and Jef D. Boeke; W$ I' R2 M7 Z" L' i+ `8 y
2 Measuring Dynamic Changes in Histone Modifications and Nucleosome
5 D& Z/ ~$ T4 W9 S6 u$ g6 {& lDensity during Activated Transcription in Budding Yeast . . . . . . . . . . . . . . . . . . . . 15
3 h4 g. s+ D, A/ r. GChhabi K. Govind, Daniel Ginsburg, and Alan G. Hinnebusch
5 ]+ c; A  g& P; W3 Monitoring the Effects of Chromatin Remodelers on Long-Range  @6 v  g8 u: X7 J
Interactions In Vivo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
1 l: m) M4 A. L; A/ `. KChristine M. Kiefer and Ann Dean' F0 G1 I: F/ D
4 Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease . . . . . . . . . . . 47# Q/ g0 z3 u/ F; A- V% Z
Gene O. Bryant
/ q) o& Q5 T7 A5 Analysis of Nucleosome Positioning Using a Nucleosome-Scanning Assay. . . . . . . . 63
1 e" L% G0 ]7 |Juan Jose Infante, G. Lynn Law, and Elton T. Young' _: F; r, H4 n, Y: B0 H5 ~& r
6 Assaying Chromatin Structure and Remodeling by Restriction Enzyme
: B6 s% I# S# {) N8 t" h  x2 J, O& z, _Accessibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
1 y% U8 c4 e8 F& w& D; Y7 F# \8 ]Kevin W. Trotter and Trevor K. Archer" ]3 t# @! T( E# N
7 Generation of DNA Circles in Yeast by Inducible Site-Specific
" Y: a& R" v+ R" fRecombination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103, t/ X$ ]3 F" ^
Marc R. Gartenberg
$ h' B! d$ @* E/ \0 U, V) I8 An Efficient Purification System for Native Minichromosome
1 L: I: Q' ~) G: M5 p0 X+ bfrom Saccharomyces cerevisiae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
; `% a+ Y8 C3 b3 K! h& A1 r3 \Ashwin Unnikrishnan, Bungo Akiyoshi, Sue Biggins, and Toshio Tsukiyama
9 G3 l9 }9 k8 Q0 [! G  |7 Z9 Simultaneous Single-Molecule Detection of Endogenous C-5 DNA& H, j7 [5 n2 v0 M' J1 W& y0 u, a
Methylation and Chromatin Accessibility Using MAPit. . . . . . . . . . . . . . . . . . . . . . 125( q% q( B+ M) ^% s# Q
Russell P. Darst, Carolina E. Pardo, Santhi Pondugula,
* g. D1 c4 j' m: l  R2 _Vamsi K. Gangaraju, Nancy H. Nabilsi, Blaine Bartholomew,
! M( `" ^5 m$ x: Q' G; o. Xand Michael P. Kladde# I9 u4 N& [1 Z% _. j
10 Analysis of Stable and Transient Protein–Protein Interactions . . . . . . . . . . . . . . . . . 1431 @, |, N) L0 ~( z+ R+ T
Stephanie Byrum, Sherri K. Smart, Signe Larson, and Alan J. Tackett! m( [9 Y6 M$ w- b
11 Monitoring Dynamic Binding of Chromatin Proteins In Vivo! p; d1 _. U/ I# i6 W% }
by Fluorescence Recovery After Photobleaching . . . . . . . . . . . . . . . . . . . . . . . . . . . 153  s  p0 b" k7 y
Florian Mueller, Tatiana S. Karpova, Davide Mazza,8 F7 r' P: N9 s5 u/ L
and James G. McNally; a2 @$ H; X* A/ K: i
12 Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence. S2 n/ B3 Y$ r* k
Correlation Spectroscopy and Temporal Image Correlation Spectroscopy . . . . . . . . 1774 c) T* R+ O* u1 N- ]# z
Davide Mazza, Timothy J. Stasevich, Tatiana S. Karpova,: x$ m$ c4 L) p/ J$ i3 ]5 W8 M; @
and James G. McNally# A. d5 W! ?$ n
13 Analysis of Chromatin Structure in Plant Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . 201
+ h# K9 C: C1 L) S% YMala Singh, Amol Ranjan, Krishan Mohan Rai, Sunil Kumar Singh," E4 c% j* W* `; G; |/ X  E; _4 ?
Verandra Kumar, Ila Trivedi, Niraj Lodhi, and Samir V. Sawant
$ z* e1 U4 L& O14 Analysis of Histones and Histone Variants in Plants. . . . . . . . . . . . . . . . . . . . . . . . . 225
: r) {4 I" k' ]Ila Trivedi, Krishan Mohan Rai, Sunil Kumar Singh,: e- M4 ], [/ q! z
Verandra Kumar, Mala Singh, Amol Ranjan, Niraj Lodhi,
5 s5 b! ^' K! ?, |0 ?. C, j. L  a  ?and Samir V. Sawant) L( d" ?0 b9 {: H# P
15 Reconstitution of Modified Chromatin Templates for In Vitro2 L3 E9 N$ Q% d
Functional Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 237
& m* k) \, b# J% h, F* @Miyong Yun, Chun Ruan, Jae-Wan Huh, and Bing Li
! J+ @! o, w, |8 Q5 W: t2 }: L0 f6 J16 A Defined In Vitro System to Study ATP-Dependent Remodeling
% [; o4 Q/ L6 @, jof Short Chromatin Fibers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
% F8 I9 x( O2 B8 @9 V4 W& n; J! s, GVerena K. Maier and Peter B. Becker) h5 s7 ^6 {3 Y/ \/ n3 `+ V, Q
17 In Vitro Reconstitution of In Vivo-Like Nucleosome Positioning) Z/ p' f! W$ i7 G" L( a6 s- [
on Yeast DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2712 C! T' D* x2 \  i5 e* y3 E+ C5 L
Christian J. Wippo and Philipp Korber- A( B( |9 t5 C) P0 ^% ~
18 Activator-Dependent Acetylation of Chromatin Model Systems. . . . . . . . . . . . . . . . 289, ^  k- S/ J$ W
Heather J. Szerlong and Jeffrey C. Hansen; W3 C3 e/ M, [& O
19 Mapping Assembly Favored and Remodeled Nucleosome Positions& _8 Q* u& C; L% ]0 t5 @9 r! f; L
on Polynucleosomal Templates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 311
4 _: ]1 l3 {- Z/ sHillel I. Sims, Chuong D. Pham, and Gavin R. Schnitzler  I- i0 N( d8 W0 L+ x3 S! M
20 Analysis of Changes in Nucleosome Conformation Using Fluorescence
% ]" W$ S9 E+ k6 A. l, y# FResonance Energy Transfer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 337, Q. t; v/ E" ?! f' J
Tina Shahian and Geeta J. Narlikar  V# o' y8 v9 D2 e
21 Preparation of Nucleosomes Containing a Specific H2A–H2A Cross-Link) Q; h$ [/ U0 t+ D+ G/ z0 g
Forming a DNA-Constraining Loop Structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . 351
6 _- V2 h# C& o/ M5 N5 D& ZNing Liu and Jeffrey J. Hayes' u1 a4 Q: B# R+ {- L, S# [
22 Sulfyhydryl-Reactive Site-Directed Cross-Linking as a Method for Probing
7 p- e7 J: o/ N& ]. gthe Tetrameric Structure of Histones H3 and H4 . . . . . . . . . . . . . . . . . . . . . . . . . . 373, `$ m. T, c  ?% s: R; g2 T& m1 F
Andrew Bowman and Tom Owen-Hughes
- b: Y) s- _  F% }* C23 Genomic Approaches for Determining Nucleosome Occupancy in Yeast . . . . . . . . . 389
" g# {8 u0 H: J( O5 D5 S3 U7 vKyle Tsui, Tanja Durbic, Marinella Gebbia, and Corey Nislow
' h, H! Z+ h1 h24 Genome-Wide Approaches to Determining Nucleosome Occupancy
! j  _7 i/ J/ [$ J- D) Zin Metazoans Using MNase-Seq . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 413. w9 z5 h4 f4 r1 a  u
Kairong Cui and Keji Zhao4 w5 n% |$ @. X* M7 H% R' x; J; h
25 Salt Fractionation of Nucleosomes for Genome-Wide Profiling . . . . . . . . . . . . . . . . 421
; [8 Z7 u3 @+ c& S, ]6 K9 WSheila S. Teves and Steven Henikoff1 e4 g& b5 b. T+ u* S
26 Quantitative Analysis of Genome-Wide Chromatin Remodeling . . . . . . . . . . . . . . . 4335 S4 ]1 P1 V0 y
Songjoon Baek, Myong-Hee Sung, and Gordon L. Hager
2 {) {* ]1 T8 W% K27 Computational Analysis of Nucleosome Positioning . . . . . . . . . . . . . . . . . . . . . . . . 4434 |2 V/ |) T" \5 W5 V
Itay Tirosh( J6 r: a5 f) W6 ~
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 451
7 t" }" W& i0 M* q4 }/ a( F
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发表于 2026-11-1 11:18 |只看该作者
说的真有道理啊!

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发表于 2026-10-29 21:50 |只看该作者
谢谢分享了!  

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发表于 2026-10-22 01:05 |只看该作者
感觉好像在哪里看过了,汗~  

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发表于 2026-10-21 08:10 |只看该作者
回贴赚学识,不错了  

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发表于 2026-10-20 18:46 |只看该作者
楼主good  

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发表于 2026-10-18 17:58 |只看该作者
看或者不看,贴子就在这里,不急不忙  

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发表于 2026-10-15 06:29 |只看该作者
我顶啊。接着顶  

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发表于 2026-8-23 19:47 |只看该作者
越办越好~~~~~~~~~`  

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发表于 2026-8-19 22:25 |只看该作者
今天没事来逛逛  
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