|

- 积分
- 56
- 威望
- 56
- 包包
- 422
|

Chromatin Remodeling
1 e* }5 A2 i0 S* j# M* ^1 v5 I, K& J) N
Contents6 r7 ^& {" V# c. Q$ v
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v- S$ R6 ]' M8 N, a3 }% {, j6 ?* _/ e
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 J) x5 w: p9 R# e1 Strain Construction and Screening Methods for a Yeast Histone H3/H4; n7 l- n1 M6 ?6 x5 |
Mutant Library. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1) I, [7 g: r" q1 |/ P: @
Junbiao Dai and Jef D. Boeke
: `: t# ]2 e7 |- y7 B6 L2 Measuring Dynamic Changes in Histone Modifications and Nucleosome
Q9 |( p: L8 `Density during Activated Transcription in Budding Yeast . . . . . . . . . . . . . . . . . . . . 157 w. h/ I$ n1 T2 g. Q* V1 W
Chhabi K. Govind, Daniel Ginsburg, and Alan G. Hinnebusch
. _& ?" {" F+ D+ q1 B4 [3 Monitoring the Effects of Chromatin Remodelers on Long-Range
( I9 e. f. `# {# FInteractions In Vivo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
2 \3 X( g$ Q @" C# NChristine M. Kiefer and Ann Dean
" q2 M5 @8 E- j$ d$ e* W( q4 Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease . . . . . . . . . . . 47
7 P( I/ c: S: h, Q hGene O. Bryant% i1 z+ ^8 |* m
5 Analysis of Nucleosome Positioning Using a Nucleosome-Scanning Assay. . . . . . . . 637 I! U4 I4 }$ S5 v& O. t8 x
Juan Jose Infante, G. Lynn Law, and Elton T. Young
: v/ U; m9 U; R2 l7 t6 Assaying Chromatin Structure and Remodeling by Restriction Enzyme
^/ h+ T; h6 ~, YAccessibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 890 T" U5 A; P- s" p U' Y- n
Kevin W. Trotter and Trevor K. Archer$ g0 X9 u0 X+ R9 B% Q7 {
7 Generation of DNA Circles in Yeast by Inducible Site-Specific
3 C" _- ^) { @ \8 N' {$ uRecombination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
1 @2 i$ D4 O1 h) N4 OMarc R. Gartenberg
& l! `& U/ `" Y$ v' `8 g9 ^8 An Efficient Purification System for Native Minichromosome
# ^/ N& ~+ {9 S. e- ]from Saccharomyces cerevisiae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
1 ~$ L+ ], i! PAshwin Unnikrishnan, Bungo Akiyoshi, Sue Biggins, and Toshio Tsukiyama( O0 p* Z3 p0 _$ E) u5 x
9 Simultaneous Single-Molecule Detection of Endogenous C-5 DNA' y7 @ f" P& p$ d
Methylation and Chromatin Accessibility Using MAPit. . . . . . . . . . . . . . . . . . . . . . 125* @: X& o; Q4 K0 G: r
Russell P. Darst, Carolina E. Pardo, Santhi Pondugula,9 n1 F8 F1 P' k: {
Vamsi K. Gangaraju, Nancy H. Nabilsi, Blaine Bartholomew,
& N7 t$ x, @* R# x) Land Michael P. Kladde
. \- |/ I0 j) w10 Analysis of Stable and Transient Protein–Protein Interactions . . . . . . . . . . . . . . . . . 143
2 b$ J0 B5 b- t7 L" _8 zStephanie Byrum, Sherri K. Smart, Signe Larson, and Alan J. Tackett5 a4 j) c1 n! j+ S
11 Monitoring Dynamic Binding of Chromatin Proteins In Vivo* \; ~ b& |8 g2 s* p) m
by Fluorescence Recovery After Photobleaching . . . . . . . . . . . . . . . . . . . . . . . . . . . 153% d& n" k$ ?- N- `* J; h: J
Florian Mueller, Tatiana S. Karpova, Davide Mazza,- C% \; F# h0 Y6 D" i2 M K
and James G. McNally
6 X3 _. b+ ?& I3 ?- y; \/ P9 V: { e12 Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence
( d8 F$ p3 z, B, e' eCorrelation Spectroscopy and Temporal Image Correlation Spectroscopy . . . . . . . . 177- ^5 y& R. ?" p1 w% h ~
Davide Mazza, Timothy J. Stasevich, Tatiana S. Karpova,
4 J8 q0 N8 r% L. f( K3 Mand James G. McNally
! h2 a* d2 E; h: f9 \( t13 Analysis of Chromatin Structure in Plant Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . 201
6 z" ^# d N% p- |+ V, u* i' L/ uMala Singh, Amol Ranjan, Krishan Mohan Rai, Sunil Kumar Singh,5 Y6 D, Y. Q5 b
Verandra Kumar, Ila Trivedi, Niraj Lodhi, and Samir V. Sawant8 @/ I3 [3 B1 I% c* A# _# b
14 Analysis of Histones and Histone Variants in Plants. . . . . . . . . . . . . . . . . . . . . . . . . 2259 d( F5 k3 }& z; i2 H
Ila Trivedi, Krishan Mohan Rai, Sunil Kumar Singh,8 L& h7 s/ _! P4 z. B+ F2 ~1 \0 \
Verandra Kumar, Mala Singh, Amol Ranjan, Niraj Lodhi,
2 M$ B b9 M# t; R, |7 n, s8 cand Samir V. Sawant
! M0 @! J& e6 I3 s( O15 Reconstitution of Modified Chromatin Templates for In Vitro) L. _+ f' u( t- F! K
Functional Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 237
1 K9 |; R1 i9 d1 k. N$ YMiyong Yun, Chun Ruan, Jae-Wan Huh, and Bing Li$ e% r) q' B1 F4 k
16 A Defined In Vitro System to Study ATP-Dependent Remodeling+ W, W& a0 K- ]0 S. O1 O
of Short Chromatin Fibers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
% h: n* ?2 X. G9 P! X% R" }Verena K. Maier and Peter B. Becker8 u9 q4 M; ~2 d# _3 g
17 In Vitro Reconstitution of In Vivo-Like Nucleosome Positioning2 O1 _+ W) F. B* {
on Yeast DNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
7 {; a8 D P6 t9 [ @& L& bChristian J. Wippo and Philipp Korber
/ M! X! @. W; N/ L. k- { g18 Activator-Dependent Acetylation of Chromatin Model Systems. . . . . . . . . . . . . . . . 2891 z8 O! J9 {; E z, U' ^
Heather J. Szerlong and Jeffrey C. Hansen$ s. E; W) ^# p9 I# n! Q5 @" X
19 Mapping Assembly Favored and Remodeled Nucleosome Positions8 Q& Z3 K3 X3 E" V: O+ ^* |( U
on Polynucleosomal Templates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3115 F- V( C9 E4 ]6 }; R4 D% `
Hillel I. Sims, Chuong D. Pham, and Gavin R. Schnitzler. y: O4 ^1 w& c" ^4 r2 c
20 Analysis of Changes in Nucleosome Conformation Using Fluorescence# |) h) Y+ b; D0 U0 z0 N
Resonance Energy Transfer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 337; t5 h! f5 P2 E, i
Tina Shahian and Geeta J. Narlikar9 M; G: X4 [: {: U* H8 e
21 Preparation of Nucleosomes Containing a Specific H2A–H2A Cross-Link
% e& I8 g/ z1 i- u* qForming a DNA-Constraining Loop Structure . . . . . . . . . . . . . . . . . . . . . . . . . . . . 351
! V# ^7 m3 D; w" i9 Z" VNing Liu and Jeffrey J. Hayes
. B! u! Y! A- H6 h; S5 `$ i% K22 Sulfyhydryl-Reactive Site-Directed Cross-Linking as a Method for Probing
8 @( B* n1 s# ~! b1 B) h8 }the Tetrameric Structure of Histones H3 and H4 . . . . . . . . . . . . . . . . . . . . . . . . . . 373 j! V2 F/ _0 n: {
Andrew Bowman and Tom Owen-Hughes$ @; g' b# p5 T J4 T2 G
23 Genomic Approaches for Determining Nucleosome Occupancy in Yeast . . . . . . . . . 389
( W# H4 z' n5 OKyle Tsui, Tanja Durbic, Marinella Gebbia, and Corey Nislow, y2 _( {' L2 s% b! X# j5 I3 N- t; s
24 Genome-Wide Approaches to Determining Nucleosome Occupancy
- q9 [. u! F9 E' R. B$ M% ?/ Ain Metazoans Using MNase-Seq . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 413
4 X% D5 N1 p8 ~Kairong Cui and Keji Zhao
7 P( T' y2 l0 l2 ~: f4 W25 Salt Fractionation of Nucleosomes for Genome-Wide Profiling . . . . . . . . . . . . . . . . 421' s( f& @ D+ A" v i# r! z
Sheila S. Teves and Steven Henikoff
& Y( C% L9 F9 Z, O5 x4 T+ ~26 Quantitative Analysis of Genome-Wide Chromatin Remodeling . . . . . . . . . . . . . . . 4333 s: E& u7 V |! n7 D! L0 M
Songjoon Baek, Myong-Hee Sung, and Gordon L. Hager
; \% z4 n/ N" Y: O27 Computational Analysis of Nucleosome Positioning . . . . . . . . . . . . . . . . . . . . . . . . 443
; _$ w% t% A' x/ UItay Tirosh
8 ]/ l/ s, u8 Z! AIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 451
. v; a2 g. i6 a0 l* `# C R z |
附件: 你需要登录才可以下载或查看附件。没有帐号?注册
|